浙江农业学报 ›› 2022, Vol. 34 ›› Issue (4): 766-780.DOI: 10.3969/j.issn.1004-1524.2022.04.13
收稿日期:
2021-07-26
出版日期:
2022-04-25
发布日期:
2022-04-28
通讯作者:
王鸿
作者简介:
*王鸿,E-mail: wrh991130@126.com基金资助:
LI Xiaolan1(), ZHANG Rui1, HAO Lanlan1, WANG Hong1,2,*()
Received:
2021-07-26
Online:
2022-04-25
Published:
2022-04-28
Contact:
WANG Hong
摘要:
NAC(NAM-ATAF1/2-CUC2)是植物特有的具有多种生物功能的一类重要转录因子,在植物应对非生物胁迫、激素信号应答与器官形成中发挥重要作用。为了解桃NAC蛋白(PpNAC)及其在桃低温胁迫过程中的响应,采用生物信息学方法和qRT-PCR系统分析了桃NAC家族基因的成员、结构、功能和低温胁迫下的表达。结果表明,桃NAC家族共有119个成员,命名为PpNAC001~PpNAC119。PpNACs的氨基酸长度在68~862 aa,平均氨基酸长度为352 aa,分子量为7.2~96.1ku,等电点为4.3~9.5。除PpNAC119定位在染色体骨架上之外,其余基因均定位在桃的8条染色体上。基因结构和保守基序分析表明,位于同一亚族的成员基因结构相似,外显子数量和长度基本相同,并且同一亚族中的成员所含motif的种类和排列顺序基本相似,推测同一亚族的成员可能具有相同的功能,而不同亚族之间的差异可能与他们的功能特异性有关。对PpNACs蛋白启动子区2 000 bp的序列进行顺式作用元件分析,结果表明,脱落酸响应原件、光响应原件、茉莉酸甲酯响应原件、赤霉素响应原件、低温响应原件和生长素响应原件在筛选到的所有原件中占比较大。qRT-PCR结果显示,在4 ℃低温胁迫2、6 h,部分基因表达量上调不明显;低温胁迫12 h,16个NAC基因的表达量均显著上调。
中图分类号:
李小兰, 张瑞, 郝兰兰, 王鸿. 桃NAC家族基因生物信息学分析及其响应低温胁迫的表达特征[J]. 浙江农业学报, 2022, 34(4): 766-780.
LI Xiaolan, ZHANG Rui, HAO Lanlan, WANG Hong. Bioinformatics analysis of peach NAC gene family and its expression characteristics in response to low temperature stress[J]. Acta Agriculturae Zhejiangensis, 2022, 34(4): 766-780.
基因 Gene | 上游引物 Forward primer (5'-3') | 下游引物 Reverse primer (5'-3') | CDS全长 Full CDS |
---|---|---|---|
PpNAC007 | TTTACCAGGGCAAGCCACCAAAG | CCTACAGAGAACCCAATCGTCCAATC | 867 |
PpNAC009 | CACCAACTCAAACCCAAACCAAAGC | AGAAGTCGATTGTGGGCAAGGTAATG | 825 |
PpNAC015 | CTAAGGCTGGACGATTGGGTTCTG | GTTGGCTGTTGCTGTTGCTGTTG | 888 |
PpNAC025 | TTCTATCTCCGCCCGTGCTACC | TTGTGCTGCTGCTGCTCATCTC | 1 959 |
PpNAC037 | ATCGTTGCTGAAGTCGGATGATGAG | CTGATGTTCTCTGCCTGTGATCTTCC | 1 386 |
PpNAC043 | GAATGTGCCGCTGGAGGGATTAG | CCTGATGACCGTTGTTGAAGTTATTGC | 1 635 |
PpNAC046 | ACTCAACTCATCGGTTTGTGGTCTG | GCTAGTCTCTTGTTGCCTCTGCTTC | 1 350 |
PpNAC048 | GGTTGGTGAGGTTGGGCTCTTTC | GGTTCTCGTGTCGCTGTTGTAGG | 1 059 |
PpNAC050 | AACAGCATAAGCAGTCAGAAACGAATG | ATCAGGAACATCATCACACCCAACAG | 1 170 |
PpNAC051 | AAGCAGCGAAGCAGTTGGGATC | CTGGGTATGGGTTGGGTTTACTTGAG | 714 |
PpNAC052 | GCATCATCAGCATCATCAGCATCATC | GTAGTAGCAGCAGCAGCAGCAG | 1 122 |
PpNAC064 | AACCAGCCCCAACAAAGCCAAG | CACCTCCTCGTCCACCGTCTTC | 1 050 |
PpNAC074 | AAGGTTGTCTGTCAATCCACTGTCAC | AGCAAGATCATCACTGAGACGGTATTC | 1 035 |
PpNAC077 | GTGTGGACAAGCCTTCATCTGGATC | AGGTAGGACTCTGCCGTATATCTTCTC | 2 589 |
PpNAC105 | AGGATTTGCCACCTGGGTTTAGATTC | CCTCCCTCCTCCCTTCCAGTTTC | 858 |
PpNAC117 | AGGAAGAGAAGGCAATGGAGATCAAAG | GGTCCAGTCCACGCTCAATCTTG | 876 |
actin | GATTCCGGTGCCCAGAAGT | CCAGCAGCTTCCATTCCAA | 1 134 |
表1 引物信息
Table 1 Information of primers
基因 Gene | 上游引物 Forward primer (5'-3') | 下游引物 Reverse primer (5'-3') | CDS全长 Full CDS |
---|---|---|---|
PpNAC007 | TTTACCAGGGCAAGCCACCAAAG | CCTACAGAGAACCCAATCGTCCAATC | 867 |
PpNAC009 | CACCAACTCAAACCCAAACCAAAGC | AGAAGTCGATTGTGGGCAAGGTAATG | 825 |
PpNAC015 | CTAAGGCTGGACGATTGGGTTCTG | GTTGGCTGTTGCTGTTGCTGTTG | 888 |
PpNAC025 | TTCTATCTCCGCCCGTGCTACC | TTGTGCTGCTGCTGCTCATCTC | 1 959 |
PpNAC037 | ATCGTTGCTGAAGTCGGATGATGAG | CTGATGTTCTCTGCCTGTGATCTTCC | 1 386 |
PpNAC043 | GAATGTGCCGCTGGAGGGATTAG | CCTGATGACCGTTGTTGAAGTTATTGC | 1 635 |
PpNAC046 | ACTCAACTCATCGGTTTGTGGTCTG | GCTAGTCTCTTGTTGCCTCTGCTTC | 1 350 |
PpNAC048 | GGTTGGTGAGGTTGGGCTCTTTC | GGTTCTCGTGTCGCTGTTGTAGG | 1 059 |
PpNAC050 | AACAGCATAAGCAGTCAGAAACGAATG | ATCAGGAACATCATCACACCCAACAG | 1 170 |
PpNAC051 | AAGCAGCGAAGCAGTTGGGATC | CTGGGTATGGGTTGGGTTTACTTGAG | 714 |
PpNAC052 | GCATCATCAGCATCATCAGCATCATC | GTAGTAGCAGCAGCAGCAGCAG | 1 122 |
PpNAC064 | AACCAGCCCCAACAAAGCCAAG | CACCTCCTCGTCCACCGTCTTC | 1 050 |
PpNAC074 | AAGGTTGTCTGTCAATCCACTGTCAC | AGCAAGATCATCACTGAGACGGTATTC | 1 035 |
PpNAC077 | GTGTGGACAAGCCTTCATCTGGATC | AGGTAGGACTCTGCCGTATATCTTCTC | 2 589 |
PpNAC105 | AGGATTTGCCACCTGGGTTTAGATTC | CCTCCCTCCTCCCTTCCAGTTTC | 858 |
PpNAC117 | AGGAAGAGAAGGCAATGGAGATCAAAG | GGTCCAGTCCACGCTCAATCTTG | 876 |
actin | GATTCCGGTGCCCAGAAGT | CCAGCAGCTTCCATTCCAA | 1 134 |
基因名称 Gene name | 登录号 ID | 染色体位置 Chromosome location | 氨基酸数量 Number of amino acids | 分子量 Molecular weight/u | 等电点 pI |
---|---|---|---|---|---|
PpNAC001 | Prupe.1G063700.1_v2.0.a1 | Pp01:4536126..4536752+ | 208 | 24 358.46 | 8.56 |
PpNAC002 | Prupe.1G088700.1_v2.0.a1 | Pp01:6635984..6637464- | 321 | 36 564.21 | 5.95 |
PpNAC003 | Prupe.1G106100.1_v2.0.a1 | Pp01:8505856..8506968- | 370 | 41 966.56 | 4.62 |
PpNAC004 | Prupe.1G220400.1_v2.0.a1 | Pp01:23497788..23499637- | 258 | 29 426.38 | 9.21 |
PpNAC005 | Prupe.1G234600.1_v2.0.a1 | Pp01:24857188..24857640+ | 86 | 9 987.39 | 6.83 |
PpNAC006 | Prupe.1G274300.1_v2.0.a1 | Pp01:28043845..28045782+ | 482 | 53 569.82 | 6.78 |
PpNAC007 | Prupe.1G277500.1_v2.0.a1 | Pp01:28214649..28216118- | 288 | 33 195.52 | 7.01 |
PpNAC008 | Prupe.1G284800.1_v2.0.a1 | Pp01:28610413..28612621- | 225 | 25 753.79 | 4.68 |
PpNAC009 | Prupe.1G284900.1_v2.0.a1 | Pp01:28616906..28619692- | 274 | 31 599.94 | 5.25 |
PpNAC010 | Prupe.1G305300.2_v2.0.a1 | Pp01:29886383..29890011+ | 449 | 50 929.50 | 4.94 |
PpNAC011 | Prupe.1G383200.1_v2.0.a1 | Pp01:34421802..34426682+ | 419 | 46 447.18 | 6.67 |
PpNAC012 | Prupe.1G390000.1_v2.0.a1 | Pp01:34813332..34814859+ | 231 | 26 255.84 | 9.15 |
PpNAC013 | Prupe.1G432600.2_v2.0.a1 | Pp01:37026200..37029641+ | 281 | 31 892.72 | 7.16 |
PpNAC014 | Prupe.1G454900.2_v2.0.a1 | Pp01:38207406..38210365+ | 342 | 38 086.85 | 8.95 |
PpNAC015 | Prupe.1G493100.1_v2.0.a1 | Pp01:40980078..40982143+ | 295 | 33 945.12 | 8.09 |
PpNAC016 | Prupe.1G560100.1_v2.0.a1 | Pp01:45724265..45726152+ | 210 | 23 995.66 | 4.97 |
PpNAC017 | Prupe.1G575600.1_v2.0.a1 | Pp01:46889483..46889899- | 118 | 13 596.51 | 9.35 |
PpNAC018 | Prupe.1G587100.2_v2.0.a1 | Pp01:47661676..47664477- | 466 | 52 751.02 | 6.37 |
PpNAC019 | Prupe.2G049200.1_v2.0.a1 | Pp02:5659348..5661571+ | 204 | 22 296.43 | 4.27 |
PpNAC020 | Prupe.2G091300.1_v2.0.a1 | Pp02:14426671..14432723+ | 632 | 68 940.74 | 5.75 |
PpNAC021 | Prupe.2G091500.1_v2.0.a1 | Pp02:14455612..14459601+ | 590 | 66 058.21 | 4.53 |
PpNAC022 | Prupe.2G196600.1_v2.0.a1 | Pp02:23403495..23405479- | 400 | 45 230.06 | 5.77 |
PpNAC023 | Prupe.2G196800.1_v2.0.a1 | Pp02:23419271..23419609- | 87 | 10 131.63 | 5.32 |
PpNAC024 | Prupe.2G201200.1_v2.0.a1 | Pp02:23790709..23797562- | 707 | 74 800.88 | 4.72 |
PpNAC025 | Prupe.2G201300.1_v2.0.a1 | Pp02:23798315..23802572- | 652 | 70 487.90 | 4.72 |
PpNAC026 | Prupe.2G201500.1_v2.0.a1 | Pp02:23815459..23815917- | 68 | 7 161.15 | 4.44 |
PpNAC027 | Prupe.2G201600.1_v2.0.a1 | Pp02:23817016..23820462- | 323 | 35 729.10 | 5.63 |
基因名称 Gene name | 登录号 ID | 染色体位置 Chromosome location | 氨基酸数量 Number of amino acids | 分子量 Molecular weight/u | 等电点 pI |
PpNAC028 | Prupe.2G201800.1_v2.0.a1 | Pp02:23824119..23826779- | 455 | 51 326.82 | 4.67 |
PpNAC029 | Prupe.2G201900.1_v2.0.a1 | Pp02:23827555..23834195- | 457 | 51 160.40 | 4.69 |
PpNAC030 | Prupe.2G202000.1_v2.0.a1 | Pp02:23841599..23845657- | 454 | 50 978.29 | 4.56 |
PpNAC031 | Prupe.2G202200.1_v2.0.a1 | Pp02:23848561..23850429- | 491 | 55 686.99 | 4.85 |
PpNAC032 | Prupe.2G202400.2_v2.0.a1 | Pp02:23864645..23868096- | 419 | 47 395.82 | 5.02 |
PpNAC033 | Prupe.2G202500.1_v2.0.a1 | Pp02:23868318..23871265- | 444 | 51 288.55 | 4.89 |
PpNAC034 | Prupe.2G202600.1_v2.0.a1 | Pp02:23883009..23884343- | 128 | 14 531.48 | 5.96 |
PpNAC035 | Prupe.2G202700.1_v2.0.a1 | Pp02:23886403..23889133- | 432 | 50 109.05 | 4.59 |
PpNAC036 | Prupe.2G202800.1_v2.0.a1 | Pp02:23891043..23894028- | 470 | 54 263.93 | 7.63 |
PpNAC037 | Prupe.2G202900.1_v2.0.a1 | Pp02:23895351..23898373- | 461 | 53 884.37 | 7.66 |
PpNAC038 | Prupe.2G203100.1_v2.0.a1 | Pp02:23902536..23903399- | 194 | 22 756.93 | 7.80 |
PpNAC039 | Prupe.2G204700.2_v2.0.a1 | Pp02:23984542..23986033+ | 221 | 26 073.66 | 7.55 |
PpNAC040 | Prupe.2G204900.1_v2.0.a1 | Pp02:23987662..23989315+ | 183 | 21 472.20 | 4.98 |
PpNAC041 | Prupe.2G278100.1_v2.0.a1 | Pp02:27875877..27879693- | 321 | 37 065.29 | 6.09 |
PpNAC042 | Prupe.2G319900.1_v2.0.a1 | Pp02:29831471..29833093+ | 222 | 24 270.56 | 4.39 |
PpNAC043 | Prupe.2G320600.1_v2.0.a1 | Pp02:29853474..29856798- | 544 | 60 887.10 | 4.87 |
PpNAC044 | Prupe.3G014400.1_v2.0.a1 | Pp03:1026994..1031590- | 682 | 77 379.97 | 5.51 |
PpNAC045 | Prupe.3G065000.1_v2.0.a1 | Pp03:4659001..4660141- | 374 | 41 615.34 | 4.92 |
PpNAC046 | Prupe.3G111400.1_v2.0.a1 | Pp03:9281361..9282710- | 449 | 50 330.25 | 4.60 |
PpNAC047 | Prupe.3G178000.2_v2.0.a1 | Pp03:19470346..19473006- | 223 | 25 461.20 | 9.52 |
PpNAC048 | Prupe.3G264600.1_v2.0.a1 | Pp03:24890994..24892912- | 352 | 40 162.98 | 6.60 |
PpNAC049 | Prupe.3G268400.1_v2.0.a1 | Pp03:25058115..25060169+ | 398 | 44 493.70 | 6.95 |
PpNAC050 | Prupe.3G293200.2_v2.0.a1 | Pp03:26210598..26214422+ | 389 | 42 477.19 | 5.11 |
PpNAC051 | Prupe.3G310600.1_v2.0.a1 | Pp03:26977887..26979629+ | 237 | 27 315.82 | 9.36 |
PpNAC052 | Prupe.4G040900.1_v2.0.a1 | Pp04:1927075..1929036- | 373 | 41 068.51 | 8.45 |
PpNAC053 | Prupe.4G053300.1_v2.0.a1 | Pp04:2574049..2576136+ | 318 | 35 868.34 | 8.47 |
PpNAC054 | Prupe.4G106100.1_v2.0.a1 | Pp04:5496176..5498944- | 309 | 35 520.20 | 8.88 |
PpNAC055 | Prupe.4G112200.1_v2.0.a1 | Pp04:5939862..5942299- | 589 | 68 906.06 | 8.24 |
PpNAC056 | Prupe.4G123700.2_v2.0.a1 | Pp04:6748812..6751639+ | 361 | 41 057.74 | 6.94 |
PpNAC057 | Prupe.4G126800.1_v2.0.a1 | Pp04:6964173..6967801+ | 353 | 40 265.62 | 4.76 |
PpNAC058 | Prupe.4G138500.1_v2.0.a1 | Pp04:7730640..7733031+ | 322 | 37 055.31 | 6.67 |
PpNAC059 | Prupe.4G143600.1_v2.0.a1 | Pp04:8234798..8239256+ | 324 | 36 486.27 | 8.08 |
PpNAC060 | Prupe.4G151900.1_v2.0.a1 | Pp04:8685959..8686814+ | 254 | 29 516.89 | 5.08 |
PpNAC061 | Prupe.4G153700.1_v2.0.a1 | Pp04:8803185..8804066- | 293 | 33 679.65 | 4.71 |
PpNAC062 | Prupe.4G154000.1_v2.0.a1 | Pp04:8820781..8821665- | 294 | 33 821.61 | 4.60 |
PpNAC063 | Prupe.4G175800.1_v2.0.a1 | Pp04:10406603..10409389+ | 358 | 39 669.39 | 7.16 |
PpNAC064 | Prupe.4G186800.1_v2.0.a1 | Pp04:11116814..11118655+ | 349 | 39 226.16 | 8.34 |
PpNAC065 | Prupe.4G187100.1_v2.0.a1 | Pp04:11138518..11140641+ | 363 | 40 353.33 | 7.78 |
PpNAC066 | Prupe.4G230600.1_v2.0.a1 | Pp04:14769628..14771233+ | 363 | 41 370.74 | 4.69 |
PpNAC067 | Prupe.4G230900.1_v2.0.a1 | Pp04:14794681..14796205+ | 379 | 42 660.02 | 4.68 |
PpNAC068 | Prupe.4G231000.1_v2.0.a1 | Pp04:14804110..14805629+ | 381 | 42 670.04 | 4.63 |
PpNAC069 | Prupe.4G246100.1_v2.0.a1 | Pp04:16347760..16349143- | 405 | 45 283.03 | 4.72 |
PpNAC070 | Prupe.4G253600.1_v2.0.a1 | Pp04:17380187..17382157- | 385 | 44 506.96 | 6.37 |
PpNAC071 | Prupe.4G255500.1_v2.0.a1 | Pp04:17789226..17790714+ | 122 | 13 993.79 | 8.85 |
PpNAC072 | Prupe.4G279600.1_v2.0.a1 | Pp04:23605886..23608135+ | 292 | 33 718.09 | 6.11 |
PpNAC073 | Prupe.5G006200.1_v2.0.a1 | Pp05:707797..710859+ | 437 | 49 281.37 | 6.25 |
PpNAC074 | Prupe.5G040400.1_v2.0.a1 | Pp05:4430568..4433387- | 344 | 39 145.21 | 5.28 |
PpNAC075 | Prupe.5G076100.2_v2.0.a1 | Pp05:9011205..9015718- | 362 | 42 319.14 | 6.54 |
PpNAC076 | Prupe.5G131900.1_v2.0.a1 | Pp05:12690115..12692055- | 436 | 50 162.09 | 6.17 |
PpNAC077 | Prupe.5G135400.1_v2.0.a1 | Pp05:12859748..12864064- | 862 | 96 115.03 | 5.19 |
PpNAC078 | Prupe.5G146100.1_v2.0.a1 | Pp05:13431803..13433032- | 264 | 29 871.59 | 8.21 |
基因名称 Gene name | 登录号 ID | 染色体位置 Chromosome location | 氨基酸数量 Number of amino acids | 分子量 Molecular weight/u | 等电点 pI |
PpNAC079 | Prupe.5G196000.1_v2.0.a1 | Pp05:15882585..15884747+ | 360 | 40 482.70 | 7.65 |
PpNAC080 | Prupe.5G203200.1_v2.0.a1 | Pp05:16322326..16323545+ | 182 | 20 966.42 | 6.19 |
PpNAC081 | Prupe.5G221600.1_v2.0.a1 | Pp05:17242546..17244798+ | 354 | 39 694.41 | 7.00 |
PpNAC082 | Prupe.5G241300.1_v2.0.a1 | Pp05:18167430..18170519- | 261 | 29 885.22 | 5.07 |
PpNAC083 | Prupe.5G244600.1_v2.0.a1 | Pp05:18323103..18326470- | 167 | 18 054.39 | 6.17 |
PpNAC084 | Prupe.6G057300.1_v2.0.a1 | Pp06:4012836..4014128+ | 327 | 37 364.00 | 5.18 |
PpNAC085 | Prupe.6G092100.1_v2.0.a1 | Pp06:6347818..6348948+ | 376 | 43 599.37 | 4.72 |
PpNAC086 | Prupe.6G098300.1_v2.0.a1 | Pp06:6843896..6844935- | 246 | 28 556.34 | 8.85 |
PpNAC087 | Prupe.6G098400.1_v2.0.a1 | Pp06:6852172..6852417- | 81 | 9 538.84 | 5.70 |
PpNAC088 | Prupe.6G098600.1_v2.0.a1 | Pp06:6857507..6859210- | 348 | 39 831.73 | 8.18 |
PpNAC089 | Prupe.6G103600.1_v2.0.a1 | Pp06:7246334..7247707+ | 457 | 51 916.25 | 4.49 |
PpNAC090 | Prupe.6G112000.1_v2.0.a1 | Pp06:8054391..8055047+ | 219 | 25 305.55 | 9.34 |
PpNAC091 | Prupe.6G133600.1_v2.0.a1 | Pp06:10488017..10489878- | 372 | 42 101.02 | 4.84 |
PpNAC092 | Prupe.6G134000.1_v2.0.a1 | Pp06:10531506..10533341- | 372 | 42 101.02 | 4.84 |
PpNAC093 | Prupe.6G134400.1_v2.0.a1 | Pp06:10621079..10622787+ | 253 | 28 999.51 | 5.67 |
PpNAC094 | Prupe.6G138100.1_v2.0.a1 | Pp06:11223633..11228642+ | 390 | 43 747.80 | 5.88 |
PpNAC095 | Prupe.6G238600.1_v2.0.a1 | Pp06:23830614..23832830+ | 356 | 40 456.38 | 8.25 |
PpNAC096 | Prupe.7G001200.1_v2.0.a1 | Pp07:160287..162425+ | 383 | 42 910.39 | 8.23 |
PpNAC097 | Prupe.7G001400.1_v2.0.a1 | Pp07:206425..208570+ | 459 | 51 029.38 | 5.70 |
PpNAC098 | Prupe.7G013900.1_v2.0.a1 | Pp07:1953064..1958262- | 431 | 48 224.95 | 5.19 |
PpNAC099 | Prupe.7G014100.2_v2.0.a1 | Pp07:2010746..2015688+ | 503 | 56 128.13 | 4.77 |
PpNAC100 | Prupe.7G020000.1_v2.0.a1 | Pp07:2997305..3001866+ | 201 | 22 047.21 | 4.29 |
PpNAC101 | Prupe.7G061900.1_v2.0.a1 | Pp07:10096751..10098559+ | 425 | 47 682.16 | 5.06 |
PpNAC102 | Prupe.7G064900.1_v2.0.a1 | Pp07:10332541..10335805- | 326 | 37 287.93 | 5.72 |
PpNAC103 | Prupe.7G065000.1_v2.0.a1 | Pp07:10338166..10339835- | 428 | 49 202.80 | 5.91 |
PpNAC104 | Prupe.7G092400.2_v2.0.a1 | Pp07:12503352..12507065+ | 356 | 39 886.74 | 5.08 |
PpNAC105 | Prupe.7G102000.1_v2.0.a1 | Pp07:13186995..13188904- | 285 | 32 123.71 | 6.76 |
PpNAC106 | Prupe.7G166300.1_v2.0.a1 | Pp07:17014002..17018578+ | 335 | 38 649.15 | 5.00 |
PpNAC107 | Prupe.7G217000.2_v2.0.a1 | Pp07:19589218..19594669+ | 492 | 55 447.38 | 6.65 |
PpNAC108 | Prupe.8G002500.1_v2.0.a1 | Pp08:228506..230784+ | 378 | 42 895.62 | 7.29 |
PpNAC109 | Prupe.8G035300.1_v2.0.a1 | Pp08:3249694..3253129+ | 351 | 39 171.89 | 5.24 |
PpNAC110 | Prupe.8G035400.1_v2.0.a1 | Pp08:3254083..3258277+ | 351 | 39 402.11 | 5.08 |
PpNAC111 | Prupe.8G035500.1_v2.0.a1 | Pp08:3261961..3265416+ | 432 | 48 336.96 | 4.54 |
PpNAC112 | Prupe.8G045800.1_v2.0.a1 | Pp08:4838306..4842516+ | 577 | 65 599.97 | 4.91 |
PpNAC113 | Prupe.8G058100.1_v2.0.a1 | Pp08:7293635..7297369+ | 306 | 35 584.73 | 6.17 |
PpNAC114 | Prupe.8G074800.1_v2.0.a1 | Pp08:10907812..10909438- | 271 | 30 979.25 | 8.93 |
PpNAC115 | Prupe.8G095200.1_v2.0.a1 | Pp08:12759198..12760058- | 286 | 33 137.99 | 5.56 |
PpNAC116 | Prupe.8G097100.1_v2.0.a1 | Pp08:12910103..12910996+ | 297 | 34 395.46 | 5.73 |
PpNAC117 | Prupe.8G174800.1_v2.0.a1 | Pp08:17765903..17768100+ | 291 | 33 889.69 | 6.62 |
PpNAC118 | Prupe.8G221900.1_v2.0.a1 | Pp08:20115448..20119485+ | 284 | 32 867.25 | 5.64 |
PpNAC119 | Prupe.I005300.1_v2.0.a1 | scaffold_53:3766..5243- | 381 | 42512.91 | 4.61 |
表2 桃NAC家族基因成员的相关信息
Table 2 Information of NAC family genes in peach
基因名称 Gene name | 登录号 ID | 染色体位置 Chromosome location | 氨基酸数量 Number of amino acids | 分子量 Molecular weight/u | 等电点 pI |
---|---|---|---|---|---|
PpNAC001 | Prupe.1G063700.1_v2.0.a1 | Pp01:4536126..4536752+ | 208 | 24 358.46 | 8.56 |
PpNAC002 | Prupe.1G088700.1_v2.0.a1 | Pp01:6635984..6637464- | 321 | 36 564.21 | 5.95 |
PpNAC003 | Prupe.1G106100.1_v2.0.a1 | Pp01:8505856..8506968- | 370 | 41 966.56 | 4.62 |
PpNAC004 | Prupe.1G220400.1_v2.0.a1 | Pp01:23497788..23499637- | 258 | 29 426.38 | 9.21 |
PpNAC005 | Prupe.1G234600.1_v2.0.a1 | Pp01:24857188..24857640+ | 86 | 9 987.39 | 6.83 |
PpNAC006 | Prupe.1G274300.1_v2.0.a1 | Pp01:28043845..28045782+ | 482 | 53 569.82 | 6.78 |
PpNAC007 | Prupe.1G277500.1_v2.0.a1 | Pp01:28214649..28216118- | 288 | 33 195.52 | 7.01 |
PpNAC008 | Prupe.1G284800.1_v2.0.a1 | Pp01:28610413..28612621- | 225 | 25 753.79 | 4.68 |
PpNAC009 | Prupe.1G284900.1_v2.0.a1 | Pp01:28616906..28619692- | 274 | 31 599.94 | 5.25 |
PpNAC010 | Prupe.1G305300.2_v2.0.a1 | Pp01:29886383..29890011+ | 449 | 50 929.50 | 4.94 |
PpNAC011 | Prupe.1G383200.1_v2.0.a1 | Pp01:34421802..34426682+ | 419 | 46 447.18 | 6.67 |
PpNAC012 | Prupe.1G390000.1_v2.0.a1 | Pp01:34813332..34814859+ | 231 | 26 255.84 | 9.15 |
PpNAC013 | Prupe.1G432600.2_v2.0.a1 | Pp01:37026200..37029641+ | 281 | 31 892.72 | 7.16 |
PpNAC014 | Prupe.1G454900.2_v2.0.a1 | Pp01:38207406..38210365+ | 342 | 38 086.85 | 8.95 |
PpNAC015 | Prupe.1G493100.1_v2.0.a1 | Pp01:40980078..40982143+ | 295 | 33 945.12 | 8.09 |
PpNAC016 | Prupe.1G560100.1_v2.0.a1 | Pp01:45724265..45726152+ | 210 | 23 995.66 | 4.97 |
PpNAC017 | Prupe.1G575600.1_v2.0.a1 | Pp01:46889483..46889899- | 118 | 13 596.51 | 9.35 |
PpNAC018 | Prupe.1G587100.2_v2.0.a1 | Pp01:47661676..47664477- | 466 | 52 751.02 | 6.37 |
PpNAC019 | Prupe.2G049200.1_v2.0.a1 | Pp02:5659348..5661571+ | 204 | 22 296.43 | 4.27 |
PpNAC020 | Prupe.2G091300.1_v2.0.a1 | Pp02:14426671..14432723+ | 632 | 68 940.74 | 5.75 |
PpNAC021 | Prupe.2G091500.1_v2.0.a1 | Pp02:14455612..14459601+ | 590 | 66 058.21 | 4.53 |
PpNAC022 | Prupe.2G196600.1_v2.0.a1 | Pp02:23403495..23405479- | 400 | 45 230.06 | 5.77 |
PpNAC023 | Prupe.2G196800.1_v2.0.a1 | Pp02:23419271..23419609- | 87 | 10 131.63 | 5.32 |
PpNAC024 | Prupe.2G201200.1_v2.0.a1 | Pp02:23790709..23797562- | 707 | 74 800.88 | 4.72 |
PpNAC025 | Prupe.2G201300.1_v2.0.a1 | Pp02:23798315..23802572- | 652 | 70 487.90 | 4.72 |
PpNAC026 | Prupe.2G201500.1_v2.0.a1 | Pp02:23815459..23815917- | 68 | 7 161.15 | 4.44 |
PpNAC027 | Prupe.2G201600.1_v2.0.a1 | Pp02:23817016..23820462- | 323 | 35 729.10 | 5.63 |
基因名称 Gene name | 登录号 ID | 染色体位置 Chromosome location | 氨基酸数量 Number of amino acids | 分子量 Molecular weight/u | 等电点 pI |
PpNAC028 | Prupe.2G201800.1_v2.0.a1 | Pp02:23824119..23826779- | 455 | 51 326.82 | 4.67 |
PpNAC029 | Prupe.2G201900.1_v2.0.a1 | Pp02:23827555..23834195- | 457 | 51 160.40 | 4.69 |
PpNAC030 | Prupe.2G202000.1_v2.0.a1 | Pp02:23841599..23845657- | 454 | 50 978.29 | 4.56 |
PpNAC031 | Prupe.2G202200.1_v2.0.a1 | Pp02:23848561..23850429- | 491 | 55 686.99 | 4.85 |
PpNAC032 | Prupe.2G202400.2_v2.0.a1 | Pp02:23864645..23868096- | 419 | 47 395.82 | 5.02 |
PpNAC033 | Prupe.2G202500.1_v2.0.a1 | Pp02:23868318..23871265- | 444 | 51 288.55 | 4.89 |
PpNAC034 | Prupe.2G202600.1_v2.0.a1 | Pp02:23883009..23884343- | 128 | 14 531.48 | 5.96 |
PpNAC035 | Prupe.2G202700.1_v2.0.a1 | Pp02:23886403..23889133- | 432 | 50 109.05 | 4.59 |
PpNAC036 | Prupe.2G202800.1_v2.0.a1 | Pp02:23891043..23894028- | 470 | 54 263.93 | 7.63 |
PpNAC037 | Prupe.2G202900.1_v2.0.a1 | Pp02:23895351..23898373- | 461 | 53 884.37 | 7.66 |
PpNAC038 | Prupe.2G203100.1_v2.0.a1 | Pp02:23902536..23903399- | 194 | 22 756.93 | 7.80 |
PpNAC039 | Prupe.2G204700.2_v2.0.a1 | Pp02:23984542..23986033+ | 221 | 26 073.66 | 7.55 |
PpNAC040 | Prupe.2G204900.1_v2.0.a1 | Pp02:23987662..23989315+ | 183 | 21 472.20 | 4.98 |
PpNAC041 | Prupe.2G278100.1_v2.0.a1 | Pp02:27875877..27879693- | 321 | 37 065.29 | 6.09 |
PpNAC042 | Prupe.2G319900.1_v2.0.a1 | Pp02:29831471..29833093+ | 222 | 24 270.56 | 4.39 |
PpNAC043 | Prupe.2G320600.1_v2.0.a1 | Pp02:29853474..29856798- | 544 | 60 887.10 | 4.87 |
PpNAC044 | Prupe.3G014400.1_v2.0.a1 | Pp03:1026994..1031590- | 682 | 77 379.97 | 5.51 |
PpNAC045 | Prupe.3G065000.1_v2.0.a1 | Pp03:4659001..4660141- | 374 | 41 615.34 | 4.92 |
PpNAC046 | Prupe.3G111400.1_v2.0.a1 | Pp03:9281361..9282710- | 449 | 50 330.25 | 4.60 |
PpNAC047 | Prupe.3G178000.2_v2.0.a1 | Pp03:19470346..19473006- | 223 | 25 461.20 | 9.52 |
PpNAC048 | Prupe.3G264600.1_v2.0.a1 | Pp03:24890994..24892912- | 352 | 40 162.98 | 6.60 |
PpNAC049 | Prupe.3G268400.1_v2.0.a1 | Pp03:25058115..25060169+ | 398 | 44 493.70 | 6.95 |
PpNAC050 | Prupe.3G293200.2_v2.0.a1 | Pp03:26210598..26214422+ | 389 | 42 477.19 | 5.11 |
PpNAC051 | Prupe.3G310600.1_v2.0.a1 | Pp03:26977887..26979629+ | 237 | 27 315.82 | 9.36 |
PpNAC052 | Prupe.4G040900.1_v2.0.a1 | Pp04:1927075..1929036- | 373 | 41 068.51 | 8.45 |
PpNAC053 | Prupe.4G053300.1_v2.0.a1 | Pp04:2574049..2576136+ | 318 | 35 868.34 | 8.47 |
PpNAC054 | Prupe.4G106100.1_v2.0.a1 | Pp04:5496176..5498944- | 309 | 35 520.20 | 8.88 |
PpNAC055 | Prupe.4G112200.1_v2.0.a1 | Pp04:5939862..5942299- | 589 | 68 906.06 | 8.24 |
PpNAC056 | Prupe.4G123700.2_v2.0.a1 | Pp04:6748812..6751639+ | 361 | 41 057.74 | 6.94 |
PpNAC057 | Prupe.4G126800.1_v2.0.a1 | Pp04:6964173..6967801+ | 353 | 40 265.62 | 4.76 |
PpNAC058 | Prupe.4G138500.1_v2.0.a1 | Pp04:7730640..7733031+ | 322 | 37 055.31 | 6.67 |
PpNAC059 | Prupe.4G143600.1_v2.0.a1 | Pp04:8234798..8239256+ | 324 | 36 486.27 | 8.08 |
PpNAC060 | Prupe.4G151900.1_v2.0.a1 | Pp04:8685959..8686814+ | 254 | 29 516.89 | 5.08 |
PpNAC061 | Prupe.4G153700.1_v2.0.a1 | Pp04:8803185..8804066- | 293 | 33 679.65 | 4.71 |
PpNAC062 | Prupe.4G154000.1_v2.0.a1 | Pp04:8820781..8821665- | 294 | 33 821.61 | 4.60 |
PpNAC063 | Prupe.4G175800.1_v2.0.a1 | Pp04:10406603..10409389+ | 358 | 39 669.39 | 7.16 |
PpNAC064 | Prupe.4G186800.1_v2.0.a1 | Pp04:11116814..11118655+ | 349 | 39 226.16 | 8.34 |
PpNAC065 | Prupe.4G187100.1_v2.0.a1 | Pp04:11138518..11140641+ | 363 | 40 353.33 | 7.78 |
PpNAC066 | Prupe.4G230600.1_v2.0.a1 | Pp04:14769628..14771233+ | 363 | 41 370.74 | 4.69 |
PpNAC067 | Prupe.4G230900.1_v2.0.a1 | Pp04:14794681..14796205+ | 379 | 42 660.02 | 4.68 |
PpNAC068 | Prupe.4G231000.1_v2.0.a1 | Pp04:14804110..14805629+ | 381 | 42 670.04 | 4.63 |
PpNAC069 | Prupe.4G246100.1_v2.0.a1 | Pp04:16347760..16349143- | 405 | 45 283.03 | 4.72 |
PpNAC070 | Prupe.4G253600.1_v2.0.a1 | Pp04:17380187..17382157- | 385 | 44 506.96 | 6.37 |
PpNAC071 | Prupe.4G255500.1_v2.0.a1 | Pp04:17789226..17790714+ | 122 | 13 993.79 | 8.85 |
PpNAC072 | Prupe.4G279600.1_v2.0.a1 | Pp04:23605886..23608135+ | 292 | 33 718.09 | 6.11 |
PpNAC073 | Prupe.5G006200.1_v2.0.a1 | Pp05:707797..710859+ | 437 | 49 281.37 | 6.25 |
PpNAC074 | Prupe.5G040400.1_v2.0.a1 | Pp05:4430568..4433387- | 344 | 39 145.21 | 5.28 |
PpNAC075 | Prupe.5G076100.2_v2.0.a1 | Pp05:9011205..9015718- | 362 | 42 319.14 | 6.54 |
PpNAC076 | Prupe.5G131900.1_v2.0.a1 | Pp05:12690115..12692055- | 436 | 50 162.09 | 6.17 |
PpNAC077 | Prupe.5G135400.1_v2.0.a1 | Pp05:12859748..12864064- | 862 | 96 115.03 | 5.19 |
PpNAC078 | Prupe.5G146100.1_v2.0.a1 | Pp05:13431803..13433032- | 264 | 29 871.59 | 8.21 |
基因名称 Gene name | 登录号 ID | 染色体位置 Chromosome location | 氨基酸数量 Number of amino acids | 分子量 Molecular weight/u | 等电点 pI |
PpNAC079 | Prupe.5G196000.1_v2.0.a1 | Pp05:15882585..15884747+ | 360 | 40 482.70 | 7.65 |
PpNAC080 | Prupe.5G203200.1_v2.0.a1 | Pp05:16322326..16323545+ | 182 | 20 966.42 | 6.19 |
PpNAC081 | Prupe.5G221600.1_v2.0.a1 | Pp05:17242546..17244798+ | 354 | 39 694.41 | 7.00 |
PpNAC082 | Prupe.5G241300.1_v2.0.a1 | Pp05:18167430..18170519- | 261 | 29 885.22 | 5.07 |
PpNAC083 | Prupe.5G244600.1_v2.0.a1 | Pp05:18323103..18326470- | 167 | 18 054.39 | 6.17 |
PpNAC084 | Prupe.6G057300.1_v2.0.a1 | Pp06:4012836..4014128+ | 327 | 37 364.00 | 5.18 |
PpNAC085 | Prupe.6G092100.1_v2.0.a1 | Pp06:6347818..6348948+ | 376 | 43 599.37 | 4.72 |
PpNAC086 | Prupe.6G098300.1_v2.0.a1 | Pp06:6843896..6844935- | 246 | 28 556.34 | 8.85 |
PpNAC087 | Prupe.6G098400.1_v2.0.a1 | Pp06:6852172..6852417- | 81 | 9 538.84 | 5.70 |
PpNAC088 | Prupe.6G098600.1_v2.0.a1 | Pp06:6857507..6859210- | 348 | 39 831.73 | 8.18 |
PpNAC089 | Prupe.6G103600.1_v2.0.a1 | Pp06:7246334..7247707+ | 457 | 51 916.25 | 4.49 |
PpNAC090 | Prupe.6G112000.1_v2.0.a1 | Pp06:8054391..8055047+ | 219 | 25 305.55 | 9.34 |
PpNAC091 | Prupe.6G133600.1_v2.0.a1 | Pp06:10488017..10489878- | 372 | 42 101.02 | 4.84 |
PpNAC092 | Prupe.6G134000.1_v2.0.a1 | Pp06:10531506..10533341- | 372 | 42 101.02 | 4.84 |
PpNAC093 | Prupe.6G134400.1_v2.0.a1 | Pp06:10621079..10622787+ | 253 | 28 999.51 | 5.67 |
PpNAC094 | Prupe.6G138100.1_v2.0.a1 | Pp06:11223633..11228642+ | 390 | 43 747.80 | 5.88 |
PpNAC095 | Prupe.6G238600.1_v2.0.a1 | Pp06:23830614..23832830+ | 356 | 40 456.38 | 8.25 |
PpNAC096 | Prupe.7G001200.1_v2.0.a1 | Pp07:160287..162425+ | 383 | 42 910.39 | 8.23 |
PpNAC097 | Prupe.7G001400.1_v2.0.a1 | Pp07:206425..208570+ | 459 | 51 029.38 | 5.70 |
PpNAC098 | Prupe.7G013900.1_v2.0.a1 | Pp07:1953064..1958262- | 431 | 48 224.95 | 5.19 |
PpNAC099 | Prupe.7G014100.2_v2.0.a1 | Pp07:2010746..2015688+ | 503 | 56 128.13 | 4.77 |
PpNAC100 | Prupe.7G020000.1_v2.0.a1 | Pp07:2997305..3001866+ | 201 | 22 047.21 | 4.29 |
PpNAC101 | Prupe.7G061900.1_v2.0.a1 | Pp07:10096751..10098559+ | 425 | 47 682.16 | 5.06 |
PpNAC102 | Prupe.7G064900.1_v2.0.a1 | Pp07:10332541..10335805- | 326 | 37 287.93 | 5.72 |
PpNAC103 | Prupe.7G065000.1_v2.0.a1 | Pp07:10338166..10339835- | 428 | 49 202.80 | 5.91 |
PpNAC104 | Prupe.7G092400.2_v2.0.a1 | Pp07:12503352..12507065+ | 356 | 39 886.74 | 5.08 |
PpNAC105 | Prupe.7G102000.1_v2.0.a1 | Pp07:13186995..13188904- | 285 | 32 123.71 | 6.76 |
PpNAC106 | Prupe.7G166300.1_v2.0.a1 | Pp07:17014002..17018578+ | 335 | 38 649.15 | 5.00 |
PpNAC107 | Prupe.7G217000.2_v2.0.a1 | Pp07:19589218..19594669+ | 492 | 55 447.38 | 6.65 |
PpNAC108 | Prupe.8G002500.1_v2.0.a1 | Pp08:228506..230784+ | 378 | 42 895.62 | 7.29 |
PpNAC109 | Prupe.8G035300.1_v2.0.a1 | Pp08:3249694..3253129+ | 351 | 39 171.89 | 5.24 |
PpNAC110 | Prupe.8G035400.1_v2.0.a1 | Pp08:3254083..3258277+ | 351 | 39 402.11 | 5.08 |
PpNAC111 | Prupe.8G035500.1_v2.0.a1 | Pp08:3261961..3265416+ | 432 | 48 336.96 | 4.54 |
PpNAC112 | Prupe.8G045800.1_v2.0.a1 | Pp08:4838306..4842516+ | 577 | 65 599.97 | 4.91 |
PpNAC113 | Prupe.8G058100.1_v2.0.a1 | Pp08:7293635..7297369+ | 306 | 35 584.73 | 6.17 |
PpNAC114 | Prupe.8G074800.1_v2.0.a1 | Pp08:10907812..10909438- | 271 | 30 979.25 | 8.93 |
PpNAC115 | Prupe.8G095200.1_v2.0.a1 | Pp08:12759198..12760058- | 286 | 33 137.99 | 5.56 |
PpNAC116 | Prupe.8G097100.1_v2.0.a1 | Pp08:12910103..12910996+ | 297 | 34 395.46 | 5.73 |
PpNAC117 | Prupe.8G174800.1_v2.0.a1 | Pp08:17765903..17768100+ | 291 | 33 889.69 | 6.62 |
PpNAC118 | Prupe.8G221900.1_v2.0.a1 | Pp08:20115448..20119485+ | 284 | 32 867.25 | 5.64 |
PpNAC119 | Prupe.I005300.1_v2.0.a1 | scaffold_53:3766..5243- | 381 | 42512.91 | 4.61 |
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