甜瓜果实发育相关SWEET糖转运蛋白基因的鉴定与功能初步分析

doi:3969/j.issn.1004-1524.2023.02.08

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (2): 308-318.DOI: 3969/j.issn.1004-1524.2023.02.08

甜瓜果实发育相关SWEET糖转运蛋白基因的鉴定与功能初步分析

李苹芳¹^,²(), 姚协丰²^,^*(), 徐锦华², 朱凌丽², 羊杏平²^,^*()

1.浙江开放大学教学中心农学教研部, 浙江杭州 310012
2.江苏省高效园艺作物遗传改良重点实验室,江苏省农业科学院蔬菜研究所, 江苏南京 210014

收稿日期:2022-05-20 出版日期:2023-02-25 发布日期:2023-03-14
作者简介:羊杏平,E-mail: 1394654153@qq.com
*姚协丰,E-mail:yaoxiefeng@jaas.ac.cn;
李苹芳(1982—),女,江苏苏州人,博士,副研究员,研究方向为瓜类蔬菜栽培和育种,农民大学生培养。E-mail: 621856792@qq.com
通讯作者: 姚协丰,羊杏平
基金资助:
国家自然科学基金(31872130);2021年度高校国内访问学者教师专业发展项目(FX2021220);国家西甜瓜产业体系(CARS-25)

Identification and preliminary functional characterization of SWEET sugar transporters involved in fruit development of melon (Cucumis melo L.)

LI Pingfang¹^,²(), YAO Xiefeng²^,^*(), XU Jinhua², ZHU Lingli², YANG Xingping²^,^*()

1. Department of Agriculture, Teaching and Researching Center, Zhejiang Open University, Hangzhou 310012, China
2. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2022-05-20 Online:2023-02-25 Published:2023-03-14
Contact: YAO Xiefeng,YANG Xingping

摘要/Abstract

摘要：

甜瓜是我国,也是世界上最重要的夏令水果之一。甜瓜果实内所含碳水化合物的种类和数量很大程度上决定其品质和产量。SWEET(sugars will eventually be exported transporters)糖转运蛋白具有运输葡萄糖和其他寡糖的功能,最近研究表明,SWEET糖转运蛋白在果实发育中可能起调控作用。本研究从甜瓜基因组中鉴定获得18个SWEETs糖转运蛋白基因,进一步通过RT-PCR并结合实时荧光定量PCR(quantitative real-time PCR,qPCR)方法,筛选到3个SWEETs基因在整个果实发育期内或某个发育时期表达量较高。亚细胞定位显示,两个SWEETs基因(CmSWEET3,CmSWEET7a)定位在细胞膜上。进一步通过酵母表达发现,甜瓜CmSWEET7a在体外具有转运葡萄糖和果糖的功能。本研究为揭示SWEET糖转运蛋白在甜瓜果实发育过程的调控作用奠定了基础。

关键词: 甜瓜, 果实, 糖转运, SWEETs

Abstract:

Melon (Cucumis melo L.) is one of the most important summer fruits in China and worldwide. The quality and yield of melon fruits are largely determined by the types and amounts of carbohydrates contained in the fruits. SWEET(sugars will eventually be exported transporters) are a new class of proteins with the ability to transport glucose and other oligosaccharides, and recent studies have shown that SWEET sugar transporters may play a role in regulating the fruit development. In this study, 18 SWEETs genes were identified from the melon genome, and further through RT-PCR combined with quantitative real-time PCR (qPCR) methods, three SWEETs genes with high expression level were identified during the whole or certain period of fruit development stages. Subcellular localization showed that two SWEETs genes(CmSWEET3,CmSWEET7a) were localized on the cell membrane. Further cloned into a yeast vector system, it was found that CmSWEET7a has the function of transporting glucose and fructose in vitro. This study laid the foundation for revealing the regulatory role of SWEET in the development process of melon fruit.

Key words: melon, fruit, sugar transporter, SWEET

中图分类号:

S652

李苹芳, 姚协丰, 徐锦华, 朱凌丽, 羊杏平. 甜瓜果实发育相关SWEET糖转运蛋白基因的鉴定与功能初步分析[J]. 浙江农业学报, 2023, 35(2): 308-318.

LI Pingfang, YAO Xiefeng, XU Jinhua, ZHU Lingli, YANG Xingping. Identification and preliminary functional characterization of SWEET sugar transporters involved in fruit development of melon (Cucumis melo L.)[J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 308-318.

图/表 8

参考文献 38

[1]	CHEN L Q, HOU B H, LALONDE S, et al. Sugar transporters for intercellular exchange and nutrition of pathogens[J]. Nature, 2010, 468(7323): 527-532.
[2]	BÜTTNER M, et al. Monosaccharide transporters in plants: structure, function and physiology[J]. Biochimica et Biophysica Acta (BBA) -Biomembranes, 2000, 1465(1/2): 263-274.
[3]	KÜHN C, et al. Sucrose transporters of higher plants[J]. Current Opinion in Plant Biology, 2010, 13(3): 287-297.
[4]	ZHANG X Y, WANG X L, WANG X F, et al. A shift of phloem unloading from symplasmic to apoplasmic pathway is involved in developmental onset of ripening in grape berry[J]. Plant Physiology, 2006, 142(1): 220-232.
[5]	NIE P X, WANG X Y, HU L P, et al. The predominance of the apoplasmic phloem-unloading pathway is interrupted by a symplasmic pathway during Chinese jujube fruit development[J]. Plant and Cell Physiology, 2010, 51(6): 1007-1018.
[6]	ZHANG L Y, PENG Y B, PELLESCHI-TRAVIER S, et al. Evidence for apoplasmic phloem unloading in developing apple fruit[J]. Plant Physiology, 2004, 135(1): 574-586.
[7]	HU L P, SUN H H, LI R F, et al. Phloem unloading follows an extensive apoplasmic pathway in cucumber (Cucumis sativus L.) fruit from anthesis to marketable maturing stage[J]. Plant, Cell & Environment, 2011, 34(11): 1835-1848.
[8]	REN Y, GUO S G, ZHANG J, et al. A tonoplast sugar transporter underlies a sugar accumulation QTL in watermelon[J]. Plant Physiology, 2018, 176(1): 836-850.
[9]	CHEN L Q, QU X Q, HOU B H, et al. Sucrose efflux mediated by SWEET proteins as a key step for phloem transport[J]. Science, 2012, 335(6065): 207-211.
[10]	KO H Y, HO L H, NEUHAUS H E, et al. Transporter SlSWEET15 unloads sucrose from phloem and seed coat for fruit and seed development in tomato[J]. Plant Physiology, 2021, 187(4): 2230-2245.
[11]	XUAN Y H, HU Y B, CHEN L Q, et al. Functional role of oligomerization for bacterial and plant SWEET sugar transporter family[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(39): E3685-E3694.
[12]	BREIA R, CONDE A, BADIM H, et al. Plant SWEETs: from sugar transport to plant-pathogen interaction and more unexpected physiological roles[J]. Plant Physiology, 2021, 186(2): 836-852.
[13]	胡丽萍, 张峰, 徐惠, 等. 植物SWEET基因家族结构、功能及调控研究进展[J]. 生物技术通报, 2017, 33(4): 27-37.
	HU L P, ZHANG F, XU H, et al. Research advances in the structure, function and regulation of SWEET gene family in plants[J]. Biotechnology Bulletin, 2017, 33(4): 27-37. (in Chinese with English abstract)
[14]	MANCK-GÖTZENBERGER J, REQUENA N. Arbuscular mycorrhiza symbiosis induces a major transcriptional reprogramming of the potato SWEET sugar transporter family[J]. Frontiers in Plant Science, 2016, 7: 487.
[15]	陈慧敏, 李威, 马雄风, 等. 植物SWEET基因家族的相关研究进展[J]. 中国农学通报, 2017, 33(19): 34-39.
	CHEN H M, LI W, MA X F, et al. SWEET gene family in plants: research advances[J]. Chinese Agricultural Science Bulletin, 2017, 33(19): 34-39. (in Chinese with English abstract)
[16]	MA L, ZHANG D C, MIAO Q S, et al. Essential role of sugar transporter OsSWEET11 during the early stage of rice grain filling[J]. Plant and Cell Physiology, 2017, 58(5): 863-873.
[17]	MATHAN J, SINGH A, RANJAN A. Sucrose transport in response to drought and salt stress involves ABA-mediated induction of OsSWEET13 and OsSWEET15 in rice[J]. Physiologia Plantarum, 2021, 171(4): 620-637.
[18]	OLIVA R, JI C H, ATIENZA-GRANDE G, et al. Broad-spectrum resistance to bacterial blight in rice using genome editing[J]. Nature Biotechnology, 2019, 37(11): 1344-1350.
[19]	KANNO Y, OIKAWA T, CHIBA Y, et al. AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes[J]. Nature Communications, 2016, 7: 13245.
[20]	CHEN L Q, LIN I W, QU X Q, et al. A cascade of sequentially expressed sucrose transporters in the seed coat and endosperm provides nutrition for the Arabidopsis embryo[J]. The Plant Cell, 2015, 27(3): 607-619.
[21]	LIVAK K J, et al. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method[J]. Methods, 2001, 25(4): 402-408.
[22]	ANJALI A, FATIMA U, SENTHIL-KUMAR M. The ins and outs of SWEETs in plants: current understanding of the basics and their prospects in crop improvement[J]. Journal of Biosciences, 2021, 46: 100.
[23]	WEI X Y, LIU F L, CHEN C, et al. The Malus domestica sugar transporter gene family: identifications based on genome and expression profiling related to the accumulation of fruit sugars[J]. Frontiers in Plant Science, 2014, 5: 569.
[24]	ZHEN Q L, FANG T, PENG Q, et al. Developing gene-tagged molecular markers for evaluation of genetic association of apple SWEET genes with fruit sugar accumulation[J]. Horticulture Research, 2018, 5(10.1038): s41438-18.
[25]	FENG C Y, HAN J X, HAN X X, et al. Genome-wide identification, phylogeny, and expression analysis of the SWEET gene family in tomato[J]. Gene, 2015, 573(2): 261-272.
[26]	ZHANG Z, ZOU L M, REN C, et al. VvSWEET10 mediates sugar accumulation in grapes[J]. Genes, 2019, 10(4): 255.
[27]	HU L P, ZHANG F, SONG S H, et al. Genome-wide identification, characterization, and expression analysis of the SWEET gene family in cucumber[J]. Journal of Integrative Agriculture, 2017, 16(7): 1486-1501.
[28]	HUANG S W, LI R Q, ZHANG Z H, et al. The genome of the cucumber, Cucumis sativus L[J]. Nature Genetics, 2009, 41(12): 1275-1281.
[29]	申长卫, 袁敬平. 南瓜SWEET蛋白家族的全基因组鉴定与进化分析[J]. 广西植物, 2021, 41(1): 40-54.
	SHEN C W, YUAN J P. Genome-wide identification and phylogenetic analysis of SWEET protein family in pumpkin[J]. Guihaia, 2021, 41(1): 40-54. (in Chinese with English abstract)
[30]	KÜHN C, GROF C P L. Sucrose transporters of higher plants[J]. Current Opinion in Plant Biology, 2010, 13(3): 287-297.
[31]	SEO P J, PARK J M, KANG S K, et al. An Arabidopsis senescence-associated protein SAG29 regulates cell viability under high salinity[J]. Planta, 2011, 233(1): 189-200.
[32]	CHARDON F, BEDU M, CALENGE F, et al. Leaf fructose content is controlled by the vacuolar transporter SWEET17 in Arabidopsis[J]. Current Biology, 2013, 23(8): 697-702.
[33]	KLEMENS P A W, PATZKE K, DEITMER J, et al. Overexpression of the vacuolar sugar carrier AtSWEET16 modifies germination, growth, and stress tolerance in Arabidopsis[J]. Plant Physiology, 2013, 163(3): 1338-1352.
[34]	杨官显, 许海峰, 张静, 等. 苹果糖转运蛋白基因MdSWEET17的功能鉴定[J]. 植物生理学报, 2018, 54(11): 1737-1745.
	YANG G X, XU H F, ZHANG J, et al. Functional identification of a sugar transporter gene MdSWEET17 in apple[J]. Plant Physiology Journal, 2018, 54(11): 1737-1745. (in Chinese with English abstract)
[35]	NI J P, LI J M, ZHU R X, et al. Overexpression of sugar transporter gene PbSWEET4 of pear causes sugar reduce and early senescence in leaves[J]. Gene, 2020, 743: 144582.
[36]	LI Y X, LIU H, YAO X H, et al. Hexose transporter CsSWEET7a in cucumber mediates phloem unloading in companion cells for fruit development[J]. Plant Physiology, 2021, 186(1): 640-654.
[37]	SONNEWALD U. Sweets-the missing sugar efflux carriers[J]. Frontiers in Plant Science, 2011, 2: 7.
[38]	CHEN L Q. SWEET sugar transporters for phloem transport and pathogen nutrition[J]. New Phytologist, 2014, 201(4): 1150-1155.

引物名称	上游引物序列	下游引物序列
Primer name	Forward primer sequence(5'-3')	Reverse primer sequence(5'-3')
CmSWEET1	TCTTGGAAATGCAACTGCTC	GGCAATTCAACATCGTCATC
CmSWEET2	AAAGTGTGGAGTTTATGCCATTT	CCCAACATAGTTCCTATTCCATT
CmSWEET3	CTCCGCAAGTTCCTTATGGT	CAATGGGCTTCCCACTAGAT
CmSWEET5d	GGCCTCTTTCTGTCACCAGT	GCATTCCATAGAACACCCAA
CmSWEET7a	GAGGGAAGGTAAAGGGAAGG	TGTTGCGAGTCCTATCAAGC
CmSWEET7c	ATGGAAGAAGGGATCAGTGG	CCGTACAATGTCCACACCAT
CmSWEET10	TTCTTCAGCCAAACATGGAC	CGGGTGCAATAGATCAGATG
CmSWEET12a	ACGCGGATGAGTTCTTTCTT	GAGCCTTCTTGGGAGCATAG
CmSWEET12c	TCGATACTCATCACCCTGGA	AAGTGCCACCACATATGGAA
CmSWEET12d	TCGAAAGAGAGTTTCTTCCTTACA	GCAGCAGGGTTATGGGTATT
CmSWEET15	CTCCTTTGGTTGTGTCATCG	AATGAAGTGAATGGCCACAA
CmSWEET17c	AACGGAGATGATGGAGGAAG	TTGGCTTTGGAAGGCTTAAC
Actin7	TGCCCAGAAGTTCTATTCCAGC	CATAGTTGAACCACCACTGAGGAC
TOPO-SWEET7a	CAC CAT GGT TTC TTT GGT GC	TCAAGCTCCATGGACCTCAG
TOPO-SWEET3	CACCATGAGATCTCTCTACACCAT	CTAGGAGTTATTTTGATGTGGA
TOPO-SWEET17c	CACCATGGCTGCTAGTTTGAGCT	TCAAAATTTTCCATCTTCCACC
NEV-SWEET7a:	GCGGCCGC AAGCTTGTAAAAGAA AT GGT TTC TTT GGT GC	GCGGCCGCTCAAGCTCCATGGACCTC
NEV-SWEET3:	GCGGCCGC AAGCTTGTAAAAGAA ATGAGATCTCTCTACACC	GCGGCCGCCTAGGAGTTATTTTGATG

引物名称	上游引物序列	下游引物序列
Primer name	Forward primer sequence(5'-3')	Reverse primer sequence(5'-3')
CmSWEET1	TCTTGGAAATGCAACTGCTC	GGCAATTCAACATCGTCATC
CmSWEET2	AAAGTGTGGAGTTTATGCCATTT	CCCAACATAGTTCCTATTCCATT
CmSWEET3	CTCCGCAAGTTCCTTATGGT	CAATGGGCTTCCCACTAGAT
CmSWEET5d	GGCCTCTTTCTGTCACCAGT	GCATTCCATAGAACACCCAA
CmSWEET7a	GAGGGAAGGTAAAGGGAAGG	TGTTGCGAGTCCTATCAAGC
CmSWEET7c	ATGGAAGAAGGGATCAGTGG	CCGTACAATGTCCACACCAT
CmSWEET10	TTCTTCAGCCAAACATGGAC	CGGGTGCAATAGATCAGATG
CmSWEET12a	ACGCGGATGAGTTCTTTCTT	GAGCCTTCTTGGGAGCATAG
CmSWEET12c	TCGATACTCATCACCCTGGA	AAGTGCCACCACATATGGAA
CmSWEET12d	TCGAAAGAGAGTTTCTTCCTTACA	GCAGCAGGGTTATGGGTATT
CmSWEET15	CTCCTTTGGTTGTGTCATCG	AATGAAGTGAATGGCCACAA
CmSWEET17c	AACGGAGATGATGGAGGAAG	TTGGCTTTGGAAGGCTTAAC
Actin7	TGCCCAGAAGTTCTATTCCAGC	CATAGTTGAACCACCACTGAGGAC
TOPO-SWEET7a	CAC CAT GGT TTC TTT GGT GC	TCAAGCTCCATGGACCTCAG
TOPO-SWEET3	CACCATGAGATCTCTCTACACCAT	CTAGGAGTTATTTTGATGTGGA
TOPO-SWEET17c	CACCATGGCTGCTAGTTTGAGCT	TCAAAATTTTCCATCTTCCACC
NEV-SWEET7a:	GCGGCCGC AAGCTTGTAAAAGAA AT GGT TTC TTT GGT GC	GCGGCCGCTCAAGCTCCATGGACCTC
NEV-SWEET3:	GCGGCCGC AAGCTTGTAAAAGAA ATGAGATCTCTCTACACC	GCGGCCGCCTAGGAGTTATTTTGATG

基因名 Gene name	甜瓜基因组ID Melon genome IDs	拟南芥同源基因 Arabidopsis hits	E值 E-value	开放阅读框 ORF length/bp	多肽Deduced polypeptide
					长度	分子量	等电点	跨膜区域
					Length/aa	MW/ku	pI	TM
CmSWEET1	MELO3C008417.2.1	AtSWEET1	6.00×10^-94	759	252	27.7	9.25	7
CmSWEET3	MELO3C005869.2.1	AtSWEET3	3.00×10^-43	627	208	23.5	9.06	5
CmSWEET5a	MELO3C002256.2.1	AtSWEET5	2.00×10^-72	552	183	21	9.42	5
CmSWEET5b	MELO3C009951.2.1	AtSWEET5	9.00×10^-67	747	248	28.2	8.91	7
CmSWEET5c	MELO3C009950.2.1	AtSWEET5	5.00×10^-63	705	234	26	8.72	7
CmSWEET7a	MELO3C016259.2.1	AtSWEET7	3.00×10^-74	789	262	29.1	9.1	7
CmSWEET7b	MELO3C005758.2.1	AtSWEET7	3.00×10^-39	504	265	29.1	9.66	7
CmSWEET9a	MELO3C008674.2.1	AtSWEET9	4.00×10^-76	798	262	30.1	9.07	7
CmSWEET9b	MELO3C031232.2.1	AtSWEET9	4.00×10^-59	639	212	24.3	9.8	6
CmSWEET10	MELO3C026184.2.1	AtSWEET10	5.00×10^-82	891	296	33	9.23	7
CmSWEET12a	MELO3C026183.2.1	AtSWEET12	1.00×10^-79	894	297	33.3	8.85	7
CmSWEET12b	MELO3C002381.2.1	AtSWEET12	2.00×10^-72	876	291	32.9	6.44	7
CmSWEET12c	MELO3C002380.2.1	AtSWEET12	3.00×10^-38	729	242	27.4	8.97	5
CmSWEET15	MELO3C022341.2.1	AtSWEET15	2.00×10^-74	822	273	30.9	9.19	6
CmSWEET17a	MELO3C000200.2.1	AtSWEET17	2.00×10^-42	582	193	21.6	7.66	4
CmSWEET17b	MELO3C004222.2.1	AtSWEET17	1.00×10^-31	456	151	16.6	8.67	5
CmSWEET17c	MELO3C027076.2.1	AtSWEET17	2.00×10^-27	615	204	22.5	7.8	4
CmSWEET17d	MELO3C031734.2.1	AtSWEET17	4.00×10^-16	501	166	19.5	9.86	2

基因名 Gene name	甜瓜基因组ID Melon genome IDs	拟南芥同源基因 Arabidopsis hits	E值 E-value	开放阅读框 ORF length/bp	多肽Deduced polypeptide
					长度	分子量	等电点	跨膜区域
					Length/aa	MW/ku	pI	TM
CmSWEET1	MELO3C008417.2.1	AtSWEET1	6.00×10^-94	759	252	27.7	9.25	7
CmSWEET3	MELO3C005869.2.1	AtSWEET3	3.00×10^-43	627	208	23.5	9.06	5
CmSWEET5a	MELO3C002256.2.1	AtSWEET5	2.00×10^-72	552	183	21	9.42	5
CmSWEET5b	MELO3C009951.2.1	AtSWEET5	9.00×10^-67	747	248	28.2	8.91	7
CmSWEET5c	MELO3C009950.2.1	AtSWEET5	5.00×10^-63	705	234	26	8.72	7
CmSWEET7a	MELO3C016259.2.1	AtSWEET7	3.00×10^-74	789	262	29.1	9.1	7
CmSWEET7b	MELO3C005758.2.1	AtSWEET7	3.00×10^-39	504	265	29.1	9.66	7
CmSWEET9a	MELO3C008674.2.1	AtSWEET9	4.00×10^-76	798	262	30.1	9.07	7
CmSWEET9b	MELO3C031232.2.1	AtSWEET9	4.00×10^-59	639	212	24.3	9.8	6
CmSWEET10	MELO3C026184.2.1	AtSWEET10	5.00×10^-82	891	296	33	9.23	7
CmSWEET12a	MELO3C026183.2.1	AtSWEET12	1.00×10^-79	894	297	33.3	8.85	7
CmSWEET12b	MELO3C002381.2.1	AtSWEET12	2.00×10^-72	876	291	32.9	6.44	7
CmSWEET12c	MELO3C002380.2.1	AtSWEET12	3.00×10^-38	729	242	27.4	8.97	5
CmSWEET15	MELO3C022341.2.1	AtSWEET15	2.00×10^-74	822	273	30.9	9.19	6
CmSWEET17a	MELO3C000200.2.1	AtSWEET17	2.00×10^-42	582	193	21.6	7.66	4
CmSWEET17b	MELO3C004222.2.1	AtSWEET17	1.00×10^-31	456	151	16.6	8.67	5
CmSWEET17c	MELO3C027076.2.1	AtSWEET17	2.00×10^-27	615	204	22.5	7.8	4
CmSWEET17d	MELO3C031734.2.1	AtSWEET17	4.00×10^-16	501	166	19.5	9.86	2

甜瓜果实发育相关SWEET糖转运蛋白基因的鉴定与功能初步分析

Identification and preliminary functional characterization of SWEET sugar transporters involved in fruit development of melon (Cucumis melo L.)

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 38

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张均, 张博, 胡碧博, 刘京亮, 张晓宇, 李春阳, 熊盛婷, 郭彬彬, 王秀存, 马超. 小麦SWEET和SUT家族基因鉴定与表达分析[J]. 浙江农业学报, 2025, 37(9): 1825-1839.
[2]	贺世雄, 杨蕾, 齐安民, 程籍, 王敏, 李英奎, 洪林. 中间砧对3种杂柑叶片光合特性、理化指标和果实品质的影响[J]. 浙江农业学报, 2025, 37(8): 1680-1693.
[3]	张顺昌, 徐继根, 符成悦, 蒲占湑, 胡丽鹏, 吴昊, 李俊兵, 辛亮, 雷元军. 喷施氨基酸钙对红美人杂柑果皮龟裂与品质的影响[J]. 浙江农业学报, 2025, 37(8): 1706-1715.
[4]	王呈阳, 刘洁雅, 吴敏怡, 谢博伊, 洪德成, 冷锋, 吴国泉. 钙处理对涝害下寒香蜜葡萄果实品质的影响[J]. 浙江农业学报, 2025, 37(7): 1451-1458.
[5]	项缨, 丛建民, 潘丹红, 陶永刚. 春大棚有机种植不同品种番茄的生育进程分析和综合评价研究[J]. 浙江农业学报, 2025, 37(6): 1252-1261.
[6]	王丽, 陈立明, 王鹏飞, 张彬, 穆霄鹏. 有机肥配施菌肥对欧李果实品质和土壤性质的影响[J]. 浙江农业学报, 2025, 37(4): 820-830.
[7]	熊韬, 闫淼, 吴婷, 马超, 杨俊涛, 胡国智. 黄腐酸钾对甜瓜根区土壤微生态、根系形态及果实品质的影响[J]. 浙江农业学报, 2025, 37(10): 2066-2076.
[8]	俞沁佩, 孙鹂, 张淑文, 俞浙萍, 郑锡良, 戚行江. 园艺作物果实β-半乳糖苷酶研究进展[J]. 浙江农业学报, 2024, 36(9): 2184-2192.
[9]	孙鹂, 张淑文, 俞浙萍, 郑锡良, 梁森苗, 任海英, 戚行江. 腐殖酸钾对杨梅土壤改良和生长结实的影响[J]. 浙江农业学报, 2024, 36(8): 1878-1886.
[10]	朱学慧, 谢辉, 韩守安, 王敏, 白世践, 马云龙, 王艳蒙, 麦斯乐, 潘明启, 张雯. 两种植物生长调节剂对无核白鸡心葡萄果实品质的影响[J]. 浙江农业学报, 2024, 36(6): 1309-1319.
[11]	汪颖, 王尖, 冯子珊, 汪宝根, 吴新义, 鲁忠富, 孙玉燕, 董文其, 李国景, 吴晓花. 瓠瓜果实品质性状因子分析和综合评价[J]. 浙江农业学报, 2024, 36(2): 334-343.
[12]	罗莎莎, 王如月, 甄紫怡, 吴嘉龙, 徐业勇, 巴合提牙儿·克热木, 孙雅丽, 虎海防. 灌溉时间和灌溉量对杏李裂果率与果实品质的影响[J]. 浙江农业学报, 2024, 36(2): 365-372.
[13]	马玲, 张镇武, 方英姿, 吴慧欣, 邢承华. 减氮配施生物炭对椪柑生长发育与土壤特性的影响[J]. 浙江农业学报, 2024, 36(12): 2739-2747.
[14]	赵凌吉, 廖香娇, 刘德春, 胡威, 匡柳青, 宋杰, 易明亮, 刘勇, 杨莉. 桃溪蜜柚果实贮藏期有机酸含量变化及相关基因表达分析[J]. 浙江农业学报, 2024, 36(11): 2510-2520.
[15]	夏智杰, 张雷, 宋江华, 傅敏, 张立新. 安徽甜瓜和栝楼蔓枯病的病原菌鉴定及其有效药剂筛选[J]. 浙江农业学报, 2024, 36(1): 168-176.