›› 2012, Vol. 24 ›› Issue (5): 0-757.

• 论文 •    

干旱胁迫对高粱苗期抗旱生理特性的影响

解芳1,2,翟国伟2,邹桂花2,陶跃之2,*   

  1. 1浙江师范大学 化学与生命科学学院,浙江 金华 321004;2浙江省农业科学院 作物与核技术利用研究所,浙江 杭州 310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-09-24 发布日期:2012-09-24

Effect of drought stress on physiological characteristics of sorghum at seedling stage

XIE Fang;ZHAI Guo-wei;ZOU Gui-hua;TAO Yue-zhi;*   

  1. 1 College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China; 2Institute of Crops and Utilization of Nuclear Technology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-09-24 Published:2012-09-24

摘要: 从654/LTR108重组自交系群体中筛选出一个持绿性株系RIL-1和一个早衰株系RIL-2。以这两个株系及其亲本为试验材料,研究了干旱胁迫对其苗期叶片的保护酶活性和渗透调节物质的影响。结果表明,随着干旱胁迫时间的延长,RIL-1,RIL-2,654和LTR108游离脯氨酸含量均上升,且上升幅度RIL-2>LTR108>654>RIL-1;654和RIL-1叶绿素含量先升高后降低,而RIL-2和LTR108先降低后上升再降低;LTR108,654,RIL-2和RIL-1的总抗氧化能力(T-AOC)先升高后降低,但不同基因型抗氧化能力达到峰值的时间有所不同。在胁迫处理的第3天,LTR108和RIL-1的抗氧化能力最高,而654和RIL-2在第4天达到最高;LTR108,654,RIL-2和RIL-1的总超氧化物歧化酶(T-SOD)先升高后降低,且RIL-1的T-SOD变幅最明显;654,RIL-2和RIL-1的过氧化物酶(POD)活性先升高后降低,而LTR108的POD活性先降低后升高再降低,654的POD活性变幅最大。

关键词: 高粱, 苗期, 抗旱, 生理特性

Abstract: A stay-green line (RIL-1) and an early-senescence line (RIL-2) were selected from a recombinant inbred line population of 654/LTR108. Five physiological indices were investigated from the leaf samples collected from the seedlings of RIL-1, RIL-2, 654 and LTR108 under drought stress, respectively. The level of proline content increased significantly with the prolonging of drought stress. RIL-2 had the highest level of proline content, followed by LTR108, 654 and RIL-1. The chlorophyll content of 654 and RIL-1 firstly increased and then decreased while RIL-2 and LTR108 exhibited a down-up-down changing pattern. The level of T-AOC activity followed an up-down pattern for all the lines investigated. RIL-2 and LTR108 had the highest level of T-AOC activities at the 3rd day, while RIL-1 and 654 reached the highest level at the 4th day. T-SOD activity had a similar pattern as T-AOC activity. The significant change was observed for RIL-1. Different patterns were observed for the level of the POD activity among 654, RIL-1, RIL-2 and LTR108, up-down pattern for 654, RIL-1 and RIL-2 but down-up-down pattern for LTR108, respectively. The significant change was occurred for the line of 654.

Key words: Sorghum bicolor L. Meonch, seedling, drought resistance, physiological characteristic