浙江农业学报

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干旱锻炼对盐胁迫下水稻幼苗根系抗氧化酶活性的影响

  

  1. (沈阳师范大学 生命科学学院,辽宁 沈阳 110034)
  • 出版日期:2016-08-25 发布日期:2016-08-04

Effect of PEG pretreatment on antioxidant enzymes activity under salt stress in root of rice seedling

  1. (College of Life Science, Shenyang Normal University, Shengyang 110034, China)
  • Online:2016-08-25 Published:2016-08-04

摘要: 采用PEG预处理对辽星1号水稻幼苗进行干旱锻炼,研究其对盐胁迫下水稻幼苗根系的缓解作用。水稻幼苗培养1周后用10% PEG6000预处理3 d,然后复水3 d,最后用100 mmol·L-1 NaCl胁迫处理3 d。测定水稻幼苗根系的相对含水量、根系活力、丙二醛含量及抗氧化酶(SOD、POD、CAT、APX)活性。结果表明,PEG预处理的水稻幼苗根系MDA含量显著低于盐胁迫的处理(P<005),而幼苗根系相对含水量、根系活力以及抗氧化酶活性则均高于盐胁迫处理。说明PEG预处理可以提高水稻幼苗根系的耐盐性,缓解水稻幼苗在盐胁迫下所受的部分伤害。

关键词: PEG预处理, 盐胁迫, 水稻, 抗氧化酶活性

Abstract:  Effects of PEG pretreatment on rice Liaoxing 1 seedling root antioxidative activities stressed by salt stress were studied. The experiment materials were dealt with 10% PEG6000 pretreatment for 3 d, next rewatered for 3 d, and then stressed for 3 d with 100 mmol·L-1 NaCl. Water content, root activity, MDA content and antioxidant enzymes (SOD, POD, CAT, APX) activity in rice seedling roots were determined. The results showed that MDA content of PEG pretreatment in roots of rice seedling was significantly lower than that of salt stressed (P<005); However, water content, root activity and activities of antioxidant enzymes with PEG pretreatment were higher than those of salt stressed. This showed that PEG pretreatment could improve the resistance of rice seedlings and alleviate the damage of salt stress on rice seedlings.

Key words:  PEG pretreatment, salt stress, rice, antioxidant enzyme activity