基于TDLAS技术测定水稻种子呼吸的初步研究

doi:10.3969/j.issn.1004-1524.2021.11.18

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (11): 2155-2163.DOI: 10.3969/j.issn.1004-1524.2021.11.18

基于TDLAS技术测定水稻种子呼吸的初步研究

郑雯¹^,²(), 贾良权²^,^*(), 祁亨年², 王瑞琴², 赵光武¹, 袁俊²

1.浙江农林大学信息工程学院,浙江杭州 311300
2.湖州师范学院信息工程学院,浙江湖州 313000

收稿日期:2020-09-02 出版日期:2021-11-25 发布日期:2021-11-26
作者简介:*贾良权,E-mail: 02426@zjhu.edu.cn
郑雯(1996—),女,安徽滁州人,硕士研究生,主要从事光谱技术在农业领域的应用研究。E-mail: 19857184353@163.com
通讯作者: 贾良权
基金资助:
国家自然科学基金(31701512);国家自然科学基金(61772198);浙江省重点研发计划(2019C02013);湖州市公益性技术应用研究计划(2019GZ15);浙江省教育厅一般科研项目(Y201941626)

Preliminary study on measuring rice seed respiration based on TDLAS technology

ZHENG Wen¹^,²(), JIA Liangquan²^,^*(), QI Hengnian², WANG Ruiqin², ZHAO Guangwu¹, YUAN Jun²

1. College of Information Engineering, Zhejiang A&F University, Hangzhou 311300,China
2. College of Information Engineering, Huzhou University, Huzhou 313000,China

Received:2020-09-02 Online:2021-11-25 Published:2021-11-26
Contact: JIA Liangquan

摘要/Abstract

摘要：

对水稻种子呼吸产生的CO₂进行实时检测,探究水稻种子呼吸强度与各项活力指标的相关性,为通过呼吸强度测量进行种子活力的无损检测提供实验依据。利用自主设计的基于可调谐二极管激光吸收光谱(TDLAS)技术的种子呼吸检测系统,实时检测种子呼吸产生CO₂气体浓度的变化。选取梦两优黄莉占不同收获期的水稻种子,每个收获期的种子分成2份,一份进行发芽试验获取种子活力指标,另外一份经过消毒、浸泡等预处理后,放入种子呼吸容器中在密闭环境下进行呼吸强度检测。每次连续检测约10 h,每组样本重复测量3次取平均值。水稻种子分别经过0、12、24、36、48 h的浸泡,发现在一定浸泡时间内种子呼吸强度随着浸泡时间的增加而增强。在连续10 h检测后,呼吸强度最高的浸泡时间段是36 h,CO₂气体浓度达到10 151 mg·m^-3,未浸泡的干种子呼吸强度最弱,CO₂气体浓度为388 mg·m^-3;水稻种子呼吸强度与发芽率、发芽势、发芽指数、淹水3 d发芽率、田间出苗率均具有一定的相关性,相关指数最高可达到0.97、0.96、0.97、0.77及0.65。研究表明,梦两优黄莉占水稻种子在浸泡36 h左右可以达到较高的呼吸强度,呼吸强度随着呼吸时间的增加呈现先增长后趋缓状态,种子的呼吸强度与各项活力指标均呈现较高的正相关性。

关键词: 水稻, 种子活力, 浸种时间, 呼吸强度, 二氧化碳, 相关性

Abstract:

To explore the correlation between rice seed respiration intensity and various vitality indicators, and to provide experimental basis for non-destructive testing of seed vitality through respiratory intensity measurement, a self-designed seed respiration detection system based on tunable diode laser absorption spectroscopy (TDLAS) was used, real-time changes in the concentration of CO₂ produced by seed respiration were detected. The rice seeds of Mengliangyou huanglizhan at different harvest periods were selected as the materials. The seeds in each harvest period were divided into two parts. One part was subjected to a germination test to obtain seed vigor index, and the other part was sterilized and soaked before pretreatment. The respiration intensity test was carried out in a closed environment in the seed respiration container. Each continuous test was about 10 hours, and each group of samples was repeated three times to get the average value. Rice seeds were soaked for 0, 12, 24, 36, 48 h, and it was found that the respiration intensity of the seeds increased with the soaking time within a certain soaking time. After 10 hours of continuous testing, the immersion time period with the highest respiratory intensity was 36 h, and the CO₂ concentration reached 10 151 mg·m^-3. The unsoaked dry seeds had the weakest respiratory intensity, with a CO₂ concentration of 388 mg·m^-3. Rice seed respiration intensity had a certain correlation with germination rate, germination vigor, germination index, three-day flooding germination rate, and field emergence rate. The highest correlation index could reach 0.97, 0.96, 0.97, 0.77 and 0.65. Studies showed that Mengliangyou huanglizhan rice seeds could reach a high respiration intensity after soaking for about 36 hours, and the respiration intensity increased first and then slowed down with the increase of respiration time. The respiration intensity of seeds had a high positive correlation with each vitality index.

Key words: rice, seed vigor, seed soaking time, respiration intensity, carbon dioxide, correlation

中图分类号:

S330.3

郑雯, 贾良权, 祁亨年, 王瑞琴, 赵光武, 袁俊. 基于TDLAS技术测定水稻种子呼吸的初步研究[J]. 浙江农业学报, 2021, 33(11): 2155-2163.

ZHENG Wen, JIA Liangquan, QI Hengnian, WANG Ruiqin, ZHAO Guangwu, YUAN Jun. Preliminary study on measuring rice seed respiration based on TDLAS technology[J]. Acta Agriculturae Zhejiangensis, 2021, 33(11): 2155-2163.

图/表 8

参考文献 32

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编号 Number	收获时期 Harvest time	发芽率 Germination rate/%	发芽势 Germination potential/%	发芽指数 Germination index	淹水3 d发芽率 Germination rate after three days of flooding/%	田间出苗率 Field germination rate/%
A1	9月4日September 4th	87.7 a	74.0 bc	28.7 ab	85.3 a	39.0 c
A2	9月7日September 7th	79.3 b	69.7 c	26.2 b	70.7 ab	48.7 b
A3	9月9日September 9th	89.3 a	77.3 b	30.5 ab	83.3 a	50.0 b
A4	9月11日September 11th	90.3 a	85.0 a	32.1 a	90.7 a	70.0 a
A5	9月13日September 13th	86.0 a	75.7 b	28.0 ab	57.3 b	51.3 b

编号 Number	收获时期 Harvest time	发芽率 Germination rate/%	发芽势 Germination potential/%	发芽指数 Germination index	淹水3 d发芽率 Germination rate after three days of flooding/%	田间出苗率 Field germination rate/%
A1	9月4日September 4th	87.7 a	74.0 bc	28.7 ab	85.3 a	39.0 c
A2	9月7日September 7th	79.3 b	69.7 c	26.2 b	70.7 ab	48.7 b
A3	9月9日September 9th	89.3 a	77.3 b	30.5 ab	83.3 a	50.0 b
A4	9月11日September 11th	90.3 a	85.0 a	32.1 a	90.7 a	70.0 a
A5	9月13日September 13th	86.0 a	75.7 b	28.0 ab	57.3 b	51.3 b

活力指标 Vitality index	测量时间 Measure time/h										平均值 Average value
活力指标 Vitality index	1	2	3	4	5	6	7	8	9	10	平均值 Average value
发芽率Germination rate	-0.14	0.02	0.41	0.71	0.86^*	0.93^*	0.97^*	0.95^*	0.92^*	0.88^*	0.89
发芽势Germination potential	-0.10	-0.14	0.29	0.61	0.78	0.88^*	0.93^*	0.96^*	0.96^*	0.94^*	0.87
发芽指数Germination index	-0.10	-0.10	0.29	0.65	0.80^*	0.90^*	0.95^*	0.97^*	0.97^*	0.97^*	0.89
淹水3 d发芽率	-0.51	-0.53	-0.33	0.04	0.21	0.37	0.52	0.61	0.69	0.77	0.46
Germination rate after three days of flooding
田间出苗率Field germination rate	0.10	-0.13	0.17	0.38	0.49	0.55	0.57	0.62	0.64	0.65	0.56

活力指标 Vitality index	测量时间 Measure time/h										平均值 Average value
活力指标 Vitality index	1	2	3	4	5	6	7	8	9	10	平均值 Average value
发芽率Germination rate	-0.14	0.02	0.41	0.71	0.86^*	0.93^*	0.97^*	0.95^*	0.92^*	0.88^*	0.89
发芽势Germination potential	-0.10	-0.14	0.29	0.61	0.78	0.88^*	0.93^*	0.96^*	0.96^*	0.94^*	0.87
发芽指数Germination index	-0.10	-0.10	0.29	0.65	0.80^*	0.90^*	0.95^*	0.97^*	0.97^*	0.97^*	0.89
淹水3 d发芽率	-0.51	-0.53	-0.33	0.04	0.21	0.37	0.52	0.61	0.69	0.77	0.46
Germination rate after three days of flooding
田间出苗率Field germination rate	0.10	-0.13	0.17	0.38	0.49	0.55	0.57	0.62	0.64	0.65	0.56

基于TDLAS技术测定水稻种子呼吸的初步研究

Preliminary study on measuring rice seed respiration based on TDLAS technology

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