杧果转录因子BES1s家族全基因组鉴定及生物信息学分析

doi:10.3969/j.issn.1004-1524.2022.05.13

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (5): 984-994.DOI: 10.3969/j.issn.1004-1524.2022.05.13

杧果转录因子BES1s家族全基因组鉴定及生物信息学分析

夏煜琪¹^,²(), 孙宇¹^,², 刘志鑫², 孙瑞青¹^,², 杨楠¹^,², 蒲金基²^,³, 张贺¹^,²^,³^,^*()

1.海南大学植物保护学院热带作物学院,海南海口 570228
2.中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室,海南海口 571101
3.中国热带农业科学院热带生物技术研究所,海南海口 571101

收稿日期:2021-03-15 出版日期:2022-05-25 发布日期:2022-06-06
通讯作者: 张贺
作者简介:* 张贺, E-mail: atzzhef@163.com
夏煜琪(1997—),女,河北张家口人,硕士研究生,研究方向为植物保护。E-mail: xiayuqi16@163.com
基金资助:
国家重点研发计划(2019YFD1000504);中国热带农业科学院基本科研业务费(1630032022007);杧果病虫害监测与防治项目

Genome-wide identification and bioinformatics analysis of BES1 transcription factor family in mango

XIA Yuqi¹^,²(), SUN Yu¹^,², LIU Zhixin², SUN Ruiqing¹^,², YANG Nan¹^,², PU Jinji²^,³, ZHANG He¹^,²^,³^,^*()

1. College of Plant Protection, College of Tropical Crops, Hainan University, Haikou 570228, China
2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture and Rural Affairs, Haikou 571101, China
3. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China

Received:2021-03-15 Online:2022-05-25 Published:2022-06-06
Contact: ZHANG He

摘要/Abstract

摘要：

BES1/BZR1是植物中特有的一类转录因子且是油菜素内酯(BR)信号转导途径的唯一转录因子,为探讨其在植物抗病抗逆反应中的作用,本研究通过运用生物信息学研究方法分析了杧果BES1s家族成员的理化性质、结构域、蛋白质结构和在不同胁迫条件下的基因表达。研究表明,杧果BES1s家族理化性质预测结果显示,成员外显子个数相差较大;结构域预测结果显示,杧果BES1s成员均具有BES1_N结构域;系统进化分析和二级结构表明,成员可分成3组,不同组之间成员存在较大差异、蛋白质三级结构预测结果显示,存在3种结构。在qRT-PCR法测定基因表达量中发现,在胶孢炭疽菌(Cg)侵染过程中MiBES1.1和MiBES1.5持续上调表达;MiBES1.12和MiBES1.13持续下调表达。在细菌性黑斑病菌(Xcm)侵染过程中,在12 h时除MiBES1.7和MiBES1.9,其他成员上调表达;在3 h时MiBES1.1~MiBES1.5和MiBES1.11下调表达。在茉莉酸甲酯(MeJA)处理过程中,MiBES1.7、MiBES1.9和MiBES1.2持续下调表达;在48 h时,除MiBES1.5和MiBES1.11~MiBES1.13其他成员下调表达。本研究结果可为杧果BES1s家族成员基因的功能研究提供依据。

关键词: 转录因子, 杧果, 生物信息学

Abstract:

BES1/BZR1 is a unique transcription factor in the plant and the only transcription factor in the brassinolide (BR) signal transduction pathway. To explore the role BES1 plays on the stress resistance and diseases resistance in mango, we used bioinformatics methods to analyze the physicochemical properties, domains, protein structures and gene expression of BES1s family in mango. The results showed that in mango that the number of exons varies greatly, and all BES1s family members have the BES1_N domain. Evolution analysis and protein secondary structures showed that three groups were divided in BES1s family of mango, and there were significant differences among different groups. The qRT-PCR assay’s results showed that during the infection process of Cg, MiBES1.1 and MiBES1.5 were continuously up-regulated expression, and MiBES1.12 and MiBES1.13 lasted down-regulated expression; during the infection process of Xcm, mango’s BES1s family up-regulated expression at 12 h, except MiBES1.7 and MiBES1.9, and MiBES1.1~MiBES1.5 and MiBES1.11 down-regulated expression at 3 h; during the MeJA treatment process, MiBES1.7、MiBES1.9 and MiBES1.2 lasted down-regulation at 48 h, mango’s BES1s gene family down-regulated expression, except MiBES1.5 and MiBES1.11~MiBES1.13. A scientific basis is provided for the further study of the BES1s family function in mango.

Key words: transcription factor, mango, bioinformatics

中图分类号:

S667.7

夏煜琪, 孙宇, 刘志鑫, 孙瑞青, 杨楠, 蒲金基, 张贺. 杧果转录因子BES1s家族全基因组鉴定及生物信息学分析[J]. 浙江农业学报, 2022, 34(5): 984-994.

XIA Yuqi, SUN Yu, LIU Zhixin, SUN Ruiqing, YANG Nan, PU Jinji, ZHANG He. Genome-wide identification and bioinformatics analysis of BES1 transcription factor family in mango[J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 984-994.

图/表 9

表1 杧果BES1s家族成员理化性质预测

Table 1 Identification of BES1s gene family in mango

基因 Gene	氨基酸数 Number of amino acids/aa	蛋白质分子量 MW/ku	蛋白等电点 pI	亲水性平均系数 GRAVY	脂溶指数 Aliphatic index	不稳定指数 Instability index	外显子 Exon
MiBES1.1	629	71.63	5.98	-0.400	72.23	38.79	2
MiBES1.2	722	81.87	6.12	-0.502	70.11	44.91	11
MiBES1.3	697	77.81	5.60	-0.411	74.40	46.53	11
MiBES1.4	697	77.94	5.66	-0.414	74.68	46.41	10
MiBES1.5	303	33.38	9.06	-0.624	62.18	65.57	10
MiBES1.6	303	33.42	9.20	-0.669	62.18	66.06	2
MiBES1.7	326	35.26	8.38	-0.588	57.24	62.79	2
MiBES1.8	1 278	142.67	7.57	-0.434	78.42	47.88	21
MiBES1.9	321	34.63	8.62	-0.551	62.02	67.39	2
MiBES1.10	441	47.89	8.70	-0.375	64.85	55.19	6
MiBES1.11	322	34.83	8.86	-0.570	63.70	66.21	2
MiBES1.12	345	36.84	7.98	-0.482	59.19	66.70	4
MiBES1.13	652	73.06	6.59	-0.337	76.41	41.30	9

图1 杧果与番茄、辣椒、甘蓝、拟南芥、黄瓜、水稻BES1s家族成员的系统进化分析 BES1,杧果;Solyc,番茄;CA,辣椒;XP,甘蓝;AT,拟南芥;Cucsa,黄瓜;LOC,水稻。

Fig.1 Phylogenetic analysis of BES1s family members among mango and tomato, pepper, cabbage, Arabidopsis, cucumber, and rice BES1, mango;Solyc,tomato;CA, chili; XP, cabbage; AT, Arabidopsis; Cucsa, cucumber; LOC, rice.

图2 杧果BES1s家族蛋白保守结构域分析

Fig.2 Conserved domain analysis of BES1s protein in mango

图3 杧果BES1s基因家族系统进化分析和基因结构分析 A,杧果BES1s家族成员系统发育树;B,保守基序预测;C,二级结构预测。

Fig.3 phylogenetic and gene structure analysis of BES1s gene family in mango A, A phylogenetic tree of BES1s family members; B, Conservative motif prediction; C, Secondary structure prediction.

图4 杧果BES1s家族成员motif 1多序列比对

Fig.4 motif 1 of BES1s family members in mango

图5 杧果BES1s家族成员蛋白质三级结构预测

Fig.5 Prediction of protein structure of BES1s family members in mango

表2 qRT-PCR引物序列

Table 2 qRT-PCR primer sequences

基因 Gene	正向引物 Forward primer(5'→3')	反向引物 Reverse primer (5' →3')
MiBES1.1	GATGACTTGTTTGTGGCGGG	ATGTCCCCGCAATCTAGCAG
MiBES1.2	AGGATGGGATGGAGGTATCC	CCCTCGTTCACAGAAGAAGC
MiBES1.3	GCTGAATGCTGCATGGGATG	CAACAACTGCTTCCCCATGC
MiBES1.4	TCCTGATGGAACCACCTTTC	GCTGAAGATTGGGACTGAGC
MiBES1.5	AGCACCTCAACCAGGATCAC	ATCCGGAGAGGAGGAAGAGA
MiBES1.6	TTTTCCACTCCTCCGAAATG	CAACTGACGATGCATTGGAC
MiBES1.7	AAGAGACGAGAGCGCAGAAG	TAGCTGATGCAGACCCTCCT
MiBES1.8	CTACACAACGATGGGCAATG	TAGCTGACTGAGGCCTGGTT
MiBES1.9	TCTTCATTTCCAAGCCCAAC	TTACCCAACGACCAGAGTCC
MiBES1.10	AGGAAGAAGTTATAGCTGGTGGTG	ACATTCTTCATGAATCCTCTCTCC
MiBES1.11	CCACCTCTATCATCTCCAACTTCT	ATCACATTCTGGTATTGTGACAGG
MiBES1.12	TTAGAGATGAGGTTCTGTCAGGTG	AACTGGTTCTAGAGTTTCCCAGTG
MiBES1.13	TGTAGACCTTTGGAAGAGAAGGAG	CAGGAGAGATTCCTCTTTGAGAAG
Miactin	GGCAAGTCTGGTGCCAG	ACGGTATCTATCTCTTCG

图6 产物扩增结果

Fig.6 Results of product amplification 1-13, MiBES1.1-MiBES13; 14, Miactin; M, DL2 000 marker。

图7 qRT-PCR中杧果BES1s基因家族成员相对表达分析 “*”表示显著上调,“#”表示显著下调。A,胶孢炭疽菌侵染下杧果BES1s基因家族成员相对表达分析; B,细菌性黑斑病菌侵染下杧果BES1s家族成员相对表达分析; C,施用茉莉酸甲酯条件下杧果BES1s家族成员相对表达分析。

Fig.7 Relative expression analysis of BES1s gene family members of mango with qRT-PCR ‘*’ the relative expression level of table was significant up-regulated,“#” the relative expression level of table was significant down-regulated. A, relative expression analysis of BES1 gene family members under the infection of Cg in mango, B, relative expression analysis of BES1s family members under the infection of Xcm in mango; C, relative expression analysis of BES1s gene family members under the infection of MeJA in mango.

参考文献 38

[1]	黎家, 李传友. 新中国成立70年来植物激素研究进展[J]. 中国科学: 生命科学, 2019, 49(10): 1227-1281.
	LI J, LI C Y. Seventy-year major research progress in plant hormones[J]. Scientia Sinica Vitae, 2019, 49(10):1227-1281. (in Chinese with English abstract)
[2]	赵毓橘, 王玉琴. 新植物激素:油菜素内酯的发现历程、生理作用及其在农业上的应用[J]. 大自然探索, 1986(3): 133-136.
	ZHAO Y J, WANG Y Q. New plant hormone: the discovery, physiological function and application of brassinosteroid in agriculture[J]. Exploration of Nature, 1986(3): 133-136. (in Chinese)
[3]	MITCHELL J W, MANDAVA N, WORLEY J F, et al. Brassins-a new family of plant hormones from rape pollen[J]. Nature, 1970, 225(5237).
[4]	李程, 梁宝魁, 王晓峰. 油菜素内酯提高蔬菜作物抗逆性的研究进展[J]. 中国蔬菜, 2015(11): 12-18.
	LI C, LIANG B K, WANG X F. Research progress on brassinosteroids for improving stress resistance vegetable crops[J]. China Vegetables, 2015(11): 12-18. (in Chinese with English abstract)
[5]	NAKASHITA H, YASUDA M, NITTA T, et al. Brassinosteroid functions in a broad range of disease resistance in tobacco and rice[J]. The Plant Journal, 2003, 33(5): 887-898. DOI URL
[6]	XIA X, DONG H, YIN Y, et al. Brassinosteroid signaling integrates multiple pathways to release apical dominance in tomato[J]. Proceedings of the National Academy of Sciences of the United States of America, 2021, 118(11): e2004384118.
[7]	孙超, 黎家. 油菜素甾醇类激素的生物合成、代谢及信号转导[J]. 植物生理学报, 2017, 53(3): 291-307.
	SUN C, LI J. Biosynthesis, catabolism, and signal transduction of brassinosteroids[J]. Plant Physiology Journal, 2017, 53(3): 291-307. (in Chinese with English abstract) DOI URL
[8]	王梦姣, 邓百万, 杨国鹏. 拟南芥油菜素内酯的合成、修饰、信号转导及其在农作物育种中的应用研究进展[J]. 河南农业科学, 2015, 44(2): 1-6.
	WANG M J, DENG B W, YANG G P. Research progress of brassinosteroid in Arabidopsis thaliana and its application in crop breeding[J]. Journal of Henan Agricultural Sciences, 2015, 44(2): 1-6. (in Chinese with English abstract)
[9]	CHEN J N, Nolan T M, YE H X, et al. Arabidopsis WRKY46, WRKY54, and WRKY 70 transcription factors are involved in brassinosteroid-regulated plant growth and drought responses[J]. The Plant Cell, 2017, 29(6): 1425-1439.
[10]	陈雪津, 王鹏杰, 郑玉成, 等. 茶树BES1转录因子全基因组鉴定与分析[J]. 西北植物学报, 2019, 39(5): 876-885.
	CHEN X J, WANG P J, ZHENG Y C, et al. Genome-wide identification and analysis of BES1 transcription factor family in Camellia sinensis[J]. Acta Botanica Boreali-Occidentalia Sinica, 2019, 39(5), 876-885. (in Chinese with English abstract)
[11]	于好强, 孙福艾, 冯文奇, 等. 转录因子BES1/BZR1调控植物生长发育及抗逆性[J]. 遗传, 2019, 41(3): 206-214.
	YU H Q, Sun F A, FENG W Q, et al. The BES1/BZR1 transcription factors regulate growth, development and stress resistance in plants[J]. Hereditas(Beijing), 2019, 41(3), 206-214. (in Chinese with English abstract)
[12]	周晔, 赵璇, 王璐, 等. 植物BZR家族基因调控非生物胁迫应答和生长发育的研究进展[J]. 中国油料作物学报, 2020, 42(4): 499-511.
	ZHOU Y, ZHAO X, WANG l, et al. Research advances on plant BZR family genes in regulating abiotic stress response and development[J]. Chinese Journal of Oil Crop Sciences, 2020, 42(4): 499-511. (in Chinese with English abstract)
[13]	WANG Z Y, TAKESHI N, GENDRON J, et al. Nuclear-localized Bzr1 mediates brassinosteroid-induced growth and feedback suppression of brassinosteroid biosynthesis[J]. Developmental Cell, 2002, 2(4): 505-513. DOI URL
[14]	YIN Y H, VAFEADOS D, TAO Y, et al. A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis[J]. Cell, 2005, 120(2): 249-259. DOI URL
[15]	ZHAO L, GHULAM Q, LI L, et al. Genome-wide analysis of Bes1 genes in gossypium revealed their evolutionary conserved roles in brassinosteroid signaling[J]. Science China Life Sciences, 2018, 61(12): 1566-1582. DOI URL
[16]	VRIET C, RUSSINOVA E, REUZEAU C. From squalene to brassinolide: the steroid metabolic and signaling pathways across the plant kingdom[J]. Molecular Plant, 2013, 6(6): 1738-1757. DOI URL
[17]	SHE J, HAN Z F, ZHOU B, et al. Structural basis for differential recognition of brassinolide by its receptors[J]. Protein & Cell, 2013, 4(6): 475-482.
[18]	SHE J, HAN Z F, KIM T W, et al. Structural insight into brassinosteroid perception by bri1[J]. Nature: International Weekly Journal of Science, 2011, 474(7352): 472-476. DOI URL
[19]	VAN N T, PARK C R, LEE K R, et al. Bes1/Bzr1 homolog 3 cooperates with E3 Ligase AtRZF1 to regulate osmotic stress and brassinosteroid responses in Arabidopsis[J]. Journal of Experimental Botany, 2021, 72(2): 636-653. DOI URL
[20]	BAI M Y, ZHANG L Y, GAMPALA S S, et al. Functions of Osbzr1 and 14-3-3 proteins in brassinosteroid signaling in rice[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(34): 13839-13844.
[21]	WANG Z Y, BAI M Y, OH E, et al. Brassinosteroid signaling network and regulation of photomorphogenesis[J]. Annual Review of Genetics, 2012, 46: 701-724. DOI URL
[22]	胡静静, 李春金, 杨启航, 等. 甘蓝型油菜BES1转录因子家族的鉴定及比较基因组学分析[J]. 分子植物育种, 2017, 15(6): 2058-2065.
	HU J J, LI C J, YANG Q H, et al. Identification and comparative genomic analysis of the BES1 transcription factor family in Brassica napus[J]. Molecular Plant Breeding, 2017, 15(6): 2058-2065. (in Chinese with English abstract)
[23]	陈业渊, 党志国, 林电, 等. 中国杧果科学研究70年[J]. 热带作物学报, 2020, 41(10): 2034-2044.
	CHEN Y Y, DANG Z G, LIN D, et al. Mango scientific research in China in the past 70 Years[J]. Chinese Journal of Tropical Crops, 2020, 41(10): 2034-2044. (in Chinese with English abstract)
[24]	WANG P, LUO Y F, HUANG J F, et al. The genome evolution and domestication of tropical fruit mango[J]. Genome Biology, 2020, 21(1): 60. DOI URL
[25]	ZHU X G, ZHANG Q J, LI K, et al. SMRT sequencing generates the chromosome-scale reference enome of tropical fruit mango, Mangifera indica[J]. bioRxiv, 2020, Doi: https://doi.org/10.1101/2020.02.22.960880. DOI
[26]	FINN R D, COGGILL P, EBERHARDT R Y, et al. The pfam protein families database: towards a more sustainable future[J]. Nucleic Acids Research, 2016, 44(D1): D279-D285.
[27]	GASTEIGER E, GATTIKER A, HOOGLAND C, et al. Expasy: the proteomics server for in-depth protein knowledge and analysis[J]. Nucleic Acids Research, 2003, 31(13): 3784-3788. DOI URL
[28]	BAILEY T L, ELKAN C. Fitting a mixture model by expectation maximization to discover motifs in biopolymers[J]. Proceeding of the 2nd International Conference Intelligent Systems for Molecular Biology.ISMB-94, 1994, 2: 28-36.
[29]	COMBET C, BLANCHET C, GEOURJON C, et al. Nps@: network protein sequence analysis[J]. Trends in Biochemical Sciences, 2000, 25(3): 147-150. DOI URL
[30]	SAITOU N, NEI M. The neighbor-joining method: a new method for reconstructing phylogenetic trees[J]. Molecular Biology and Evolution, 1987, 4(4): 406-425.
[31]	余舜武, 刘鸿艳, 罗利军. 利用不同实时定量PCR方法分析相对基因表达差异[J]. 作物学报, 2007(7): 1214-1218.
	YU S W, LIU H Y, LUO L J. Analysis of relative gene expression using different real-time quantitative PCR[J]. Acta Agronomica Sinica, 2007(7): 1214-1218. (in Chinese with English abstract)
[32]	LU S, WANG J, CHITSAZ F, et al. CDD/SPARCLE: the conserved domain database in 2020[J]. Nucleic Acids Research, 2020, 48(D1): D265-D268. DOI URL
[33]	KANG Y N, TANABE A, ADACHI M, et al. Structural analysis of threonine 342 mutants of soybean beta-amylase: role of a conformational change of the inner loop in the catalytic mechanism[J]. Biochemistry, 2005, 44(13): 5106-5116. DOI URL
[34]	NOSAKI S, MIYAKAWA T, XU Y, et al. Structural basis for brassinosteroid response by BIL1/BZR1[J]. Nature Plants, 2018, 4(10): 771-776. DOI URL
[35]	WILLIAM R S, GILBERT W. The evolution of spliceosomal introns: patterns, puzzles and progress[J]. Nature Reviews Genetics, 2006, 7(3): 211-221. DOI URL
[36]	LIAO K, PENG Y J, YUAN L B, et al. Brassinosteroids antagonize jasmonate-activated plant defense responses through BRI1-EMS-SUPPRESSOR1(BES1)[J]. Plant Physiology, 2020, 182(2): 1066-1082. DOI URL
[37]	薛正刚, 王树杰, 冯辉, 等. 大麦BZR基因家族的全基因组鉴定及生物信息学分析[J]. 分子植物育种, 2021, 19(12): Doi: 10.13271/j.mpb.019.003849. DOI
	XUE Z G, WANG S J, FENG H, et al. Genome-wide identification and bioinformatics analysis of HvBZR gene family in Barley[J]. Molecular Plant Breeding, 2021, 19(12): Doi: 10.13271/j.mpb.019.003849. (in Chinese with English abstract) DOI
[38]	KONO A, YIN Y H. Updates on Bes1/bzr1 regulatory networks coordinating plant growth and stress responses[J]. Frontiers in Plant Science, 2020, 11: 617162. DOI URL

杧果转录因子BES1s家族全基因组鉴定及生物信息学分析

Genome-wide identification and bioinformatics analysis of BES1 transcription factor family in mango

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