不同海拔黄果柑果实有机酸代谢的生态响应

doi:10.3969/j.issn.1004-1524.2023.04.12

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (4): 853-861.DOI: 10.3969/j.issn.1004-1524.2023.04.12

不同海拔黄果柑果实有机酸代谢的生态响应

吴娇(), 龚成宇, 陈超群, 陈红旭, 刘俊宏, 唐文静, 初元琦, 杨文龙, 张瑶, 龚荣高()

四川农业大学园艺学院,四川成都 611130

收稿日期:2022-01-22 出版日期:2023-04-25 发布日期:2023-05-05
通讯作者: ^*龚荣高,E-mail: 63830947@qq.com
作者简介:吴娇(2000—),女,四川达州人,本科生,研究方向为果树栽培研究。E-mail: 3220581363@qq.com
基金资助:
国家重点研发计划项目(2017YFC0505104)

Ecological response of organic acid metabolism of Huangguogan fruits at different altitudes

WU Jiao(), GONG Chengyu, CHEN Chaoqun, CHEN Hongxu, LIU Junhong, TANG Wenjing, CHU Yuanqi, YANG Wenlong, ZHANG Yao, GONG Ronggao()

College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China

Received:2022-01-22 Online:2023-04-25 Published:2023-05-05

摘要/Abstract

摘要：

为探究大渡河干暖河谷区黄果柑果实有机酸积累与不同海拔高度的关联性,在同一山体选取了海拔为875 m、1 014 m和1 174 m的7年生黄果柑,研究有机酸代谢相关指标在果实发育过程中的变化规律。结果表明:黄果柑果实有机酸主要是柠檬酸,占比75%以上,其次是苹果酸和奎宁酸;柠檬酸在后期大量降解,并且海拔越高,柠檬酸含量越低;随着海拔升高果实柠檬酸降解阶段细胞质顺-乌头酸酶(ACO)活性和异柠檬酸脱氢酶(NADP-IDH)活性显著提升,同时海拔的升高提前启动ACO基因表达和增加NADP-IDH基因表达量。不同海拔间果实有机酸含量差异取决于果实发育中后期柠檬酸的降解量;引起不同海拔间有机酸含量差异的主要酶是细胞质ACO、NADP-IDH和磷酸烯醇式丙酮酸羧化酶(PEPC);海拔的升高可能会通过提前启动ACO基因表达和增加NADP-IDH基因表达量来提升细胞质ACO及NADP-IDH活性,以促进果实柠檬酸的降解。

关键词: 海拔, 黄果柑, 果实发育, 有机酸代谢, 响应机制, 基因表达

Abstract:

To reveal the relationship between Huangguogan fruit organic acid accumulation and different altitudes in the dry and warm valley of Dadu River, seven-year-old Huangguogan with altitudes of 875 m, 1 014 m and 1174 m were selected on the same mountain to study the changes of organic acid metabolism related indicators during fruit development. The results showed that the fruit organic acid was mainly citrate acid, accounting for more than 75%, followed by malic acid and quinic acid; the citric acid content was largely degraded in the later stage, and the higher the altitude, the lower the citric acid content; as the altitude increased significantly enhanced the cytoplasmic ACO activity and NADP-IDH activity in the citric acid degradation stage of the fruit. At the same time, the increase of altitude started ACO gene expression and increased NADP-IDH gene expression in advance.The difference in fruit organic acid content between different altitudes depended on the degradation of citric acid in the middle and late stages of fruit development; the main enzymes that caused the difference in organic acid content between different altitudes were cytoplasmic ACO, NADP-IDH and PEPC. Increased altitude might increase the activity of cytoplasmic ACO and NADP-IDH by activating ACO gene expression in advance and increasing NADP-IDH gene expression to promote the degradation of citric acid in fruit.

Key words: altitude, Huangguogan, fruit development, organic acid metabolism, response mechanism, gene expression

中图分类号:

S666.1

吴娇, 龚成宇, 陈超群, 陈红旭, 刘俊宏, 唐文静, 初元琦, 杨文龙, 张瑶, 龚荣高. 不同海拔黄果柑果实有机酸代谢的生态响应[J]. 浙江农业学报, 2023, 35(4): 853-861.

WU Jiao, GONG Chengyu, CHEN Chaoqun, CHEN Hongxu, LIU Junhong, TANG Wenjing, CHU Yuanqi, YANG Wenlong, ZHANG Yao, GONG Ronggao. Ecological response of organic acid metabolism of Huangguogan fruits at different altitudes[J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 853-861.

图/表 7

表1 不同海拔高度黄果柑园区的主要气候因子差异

Table 1 The difference of main climatic factors in Huangguogan gardens at different altitudes

海拔 Altitude/m	光照强度 Intensity of illumination/lx	年平均气温 Annual average temperature/℃	≥10 ℃年积温 ≥10 ℃ annual accumulated temperature/℃	年平均空气相对湿度 Annual average air relative humidity/%	年平均日温差 Annual mean daily temperature difference/℃
875	9.61×10⁴	18.2	6 165	58.3	7.2
1 014	9.97×10⁴	17.0	6 040	59.7	9.4
1 174	1.04×10⁵	15.7	5 781	61.9	11.5

表2 黄果柑果实有机酸代谢相关酶基因qPCR序列

Table 2 qPCR sequence of organic acid metabolism-related enzymes in Huangguogan fruit

基因 Gene	上游引物Forward primer(5'-3')	下游引物Reverse primer(5'-3')
CS	CCTGAGTGCCAGAAGTTGTT	AGAAGTTCCCACCATAATCC
PEPC	GGAATCGGATCAATGGTAAG	TGGTGTAGCCAATCGTAAAT
MDH	CTCCAACCCAACCTCACAG	GACCAAACCCAAACCAAAT
NADP-IDH	TTTTGCTGACGCTACCTTG	GGCACTCCAGTACGGATGT
ACO	GGCTTACTGCTTACTCAAC	AACTTCCACTGCCATACTC
β-actin	AGGCTCCACAAGCAAGTATIA	CATCAAGGCAGGGTTCACATT

图1 不同海拔黄果柑果实发育过程中有机酸及酸组分含量的变化同一时间不同处理间没有相同小写字母表示差异显著(P<0.05)。下同。

Fig.1 Changes of soluble total acid and acid components in Huangguogan fruit at different altitudes during fruit development The bars with different lowercase letters in the same day after flowering showed significant difference (P<0.05).The same as below.

图2 不同海拔黄果柑果实成熟期有机酸合成酶类活性的变化

Fig.2 Changes of organic acid synthase activity in Huangguogan fruit at different altitudes at mature stage

图3 不同海拔黄果柑果实成熟期有机酸分解酶类活性的变化

Fig.3 Changes of organic acid synthase activity in Huangguogan fruit at different altitudes at mature stage

图4 不同海拔黄果柑果实成熟期有机酸合成酶类基因相对表达丰度的变化

Fig.4 Changes of relative expression level of organic acid synthetic enzyme genes in Huangguogan fruit at different altitudes at mature stage

图5 不同海拔黄果柑果实成熟期有机酸分解酶类基因相对表达丰度的变化

Fig.5 Changes of relative expression level of organic acid synthetic enzyme gengs in Huangguogan fruit at different altitudes at mature stage

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不同海拔黄果柑果实有机酸代谢的生态响应

Ecological response of organic acid metabolism of Huangguogan fruits at different altitudes

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