甘薯抗茎腐病鉴定技术的建立及种质资源抗性分析

doi:10.3969/j.issn.1004-1524.20230458

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (3): 569-578.DOI: 10.3969/j.issn.1004-1524.20230458

甘薯抗茎腐病鉴定技术的建立及种质资源抗性分析

柴荣耀¹(), 游雨欣¹^,², 邱海萍¹, 郭峻宁², 张震¹, 李斌², 沈升法³, 王艳丽¹^,^*()

1.浙江省农业科学院植物保护与微生物研究所/省部共建农产品质量安全危害因子与风险防控国家重点实验室/浙江省国家农作物品种抗性鉴定试验站,浙江杭州 310021
2.浙江大学生物技术研究所,水稻生物育种全国重点实验室/农业农村部作物病虫分子生物学重点实验室/浙江省作物病虫生物学重点实验室,浙江杭州 310058
3.浙江省农业科学院作物与核技术利用研究所,浙江杭州 310021

收稿日期:2023-04-07 出版日期:2024-03-25 发布日期:2024-04-09
作者简介:柴荣耀(1966—),男,浙江余姚人,学士,副研究员,主要从事植物病理学研究。E-mail: rychai@sina.com
通讯作者: *王艳丽,E-mail: ylwang88@aliyun.com
基金资助:
浙江省重点研发计划(2017C02002);浙江省农业科学院省部共建农产品质量安全危害因子与风险防控国家重点实验室自主设计项目(2010DS700124-ZZ2014)

Establishment of sweet potato stem rot resistance identification technology and analysis of germplasm resource resistance

CHAI Rongyao¹(), YOU Yuxin¹^,², QIU Haiping¹, GUO Junning², ZHANG Zhen¹, LI Bin², SHEN Shengfa³, WANG Yanli¹^,^*()

1. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences/State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products/Zhejiang National Crop Variety Resistance Identification Experiment Station, Hangzhou 310021, China
2. State Key Laboratory of Rice Biology and Breeding, Key Laboratory of Molecular lar Biology of Crop Pathogens and Insects, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institure of Biotechnology, Zhejiang University, Hangzhou 310058, China
3. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2023-04-07 Online:2024-03-25 Published:2024-04-09

摘要/Abstract

摘要：

为优化甘薯种质抗茎腐病鉴定技术,通过分析不同的接种方法和菌液浓度对甘薯种质抗性的影响,建立甘薯抗茎腐病鉴定标准,为甘薯抗病育种奠定基础。利用组培瓶蛭石薯苗擦伤接菌法和最佳接种菌液浓度(1.1×10⁷CFU·mL^-1),对最具代表性的6个甘薯品种进行接种鉴定,并与田间抗性品种比较进行验证,室内接种抗性鉴定结果与田间抗性表现基本一致,表明新抗性鉴定技术的鉴定结果具有准确性、可靠性。进一步对65个甘薯材料进行抗病鉴定,发现绝大部分材料表现为感病或中感, 7 份种质(YD7002、DY7082-1、浙紫薯1号、TF1118、DY7032、南薯88和浙紫薯4号)对甘薯茎腐病具有稳定抗性。文章建立了甘薯抗茎腐病的新鉴定技术,鉴定和筛选出对甘薯茎腐病表现稳定抗性的种质,为甘薯抗茎腐病育种提供重要的种质资源,对于甘薯病害防控具有重要意义。

关键词: 甘薯, 茎腐病, 种质资源, 抗性鉴定

Abstract:

In order to optimize the identification technique for stem rot resistance in sweet potato germplasm, this study analyzed the effects of different inoculation methods and bacterial liquid concentrations on the resistance of sweet potato germplasm. The aim was to establish a standardized identification criterion for stem rot resistance and lay a foundation for disease-resistant breeding in sweet potato. Using the inoculation method of scratching the surface of tissue-cultured sweet potato plantlets and the optimal bacterial liquid concentration (1.1×10⁷CFU·mL^-1), six representative sweet potato varieties were inoculated and identified. The results of indoor resistance identification were validated against field resistance performance and showed a high degree of consistency, indicating the accuracy and reliability of the newly developed identification technique. Furthermore, 65 sweet potato materials were identified for resistance to stem rot. Most of the materials were susceptible or moderately susceptible. However, seven germplasm resources (YD7002, DY7082-1, Zhezi No.1, TF1118, DY7032, Nanshu 88 and Zhezi No.4) exhibited stable resistance against stem rot in sweet potato. This study established a new identification technique for stem rot resistance in sweet potato and identified germplasm resources with stable resistance, providing important genetic materials for disease-resistant breeding. These findings are of significant importance for the prevention and control of sweet potato diseases.

Key words: sweet potato, stem rot, germplasm resource, resistance identification

中图分类号:

S435.311

柴荣耀, 游雨欣, 邱海萍, 郭峻宁, 张震, 李斌, 沈升法, 王艳丽. 甘薯抗茎腐病鉴定技术的建立及种质资源抗性分析[J]. 浙江农业学报, 2024, 36(3): 569-578.

CHAI Rongyao, YOU Yuxin, QIU Haiping, GUO Junning, ZHANG Zhen, LI Bin, SHEN Shengfa, WANG Yanli. Establishment of sweet potato stem rot resistance identification technology and analysis of germplasm resource resistance[J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 569-578.

图/表 9

参考文献 23

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病情病级 Disease level	各病级对应症状 Corresponding symptoms for each disease level	抗性水平 Resistance level
0	接种处无明显症状,植物生长正常 Inoculation site showed no apparent symptoms, and plant growth was normal	高抗 Highly resistant(HR)
1	接种处轻微变褐色,但茎仍坚挺,植株生长正常 Inoculation site exhibited slight browning, but the stem remained firm, and plant growth was normal	抗病 Resistant(R)
3	接种处形成褐色病斑,病斑未绕茎,未上下扩展,根系正常生长 Inoculation site developed brown lesions without encircling the stem or expanding upwards or downwards, and the root system grew normally	中抗 Moderately resistant (MR)
5	接种处形成水渍状病斑,病斑绕茎,上下扩展,根系生长异常,植株易在接种处折断 Inoculation site developed water-soaked lesions, encircling the stem and expanding upwards and downwards, with abnormal root growth, and plants were prone to breakage at the inoculation site	中感 Moderately susceptible (MS)
7	接种处黑褐色腐烂,上下扩展,全株萎蔫 Inoculation site exhibited black-brown rot, expanding upwards and downwards, resulting in complete wilting of the entire plant	感病 Susceptible(S)
9	接种处24 h后黑褐色腐烂上下扩展,全株水渍状腐烂 Inoculation site showed black-brown rot expanding both upwards and downwards 24 h post- inoculation, resulting in water-soaked rot throughout the entire plant	高感 Highly susceptible (HS)

病情病级 Disease level	各病级对应症状 Corresponding symptoms for each disease level	抗性水平 Resistance level
0	接种处无明显症状,植物生长正常 Inoculation site showed no apparent symptoms, and plant growth was normal	高抗 Highly resistant(HR)
1	接种处轻微变褐色,但茎仍坚挺,植株生长正常 Inoculation site exhibited slight browning, but the stem remained firm, and plant growth was normal	抗病 Resistant(R)
3	接种处形成褐色病斑,病斑未绕茎,未上下扩展,根系正常生长 Inoculation site developed brown lesions without encircling the stem or expanding upwards or downwards, and the root system grew normally	中抗 Moderately resistant (MR)
5	接种处形成水渍状病斑,病斑绕茎,上下扩展,根系生长异常,植株易在接种处折断 Inoculation site developed water-soaked lesions, encircling the stem and expanding upwards and downwards, with abnormal root growth, and plants were prone to breakage at the inoculation site	中感 Moderately susceptible (MS)
7	接种处黑褐色腐烂,上下扩展,全株萎蔫 Inoculation site exhibited black-brown rot, expanding upwards and downwards, resulting in complete wilting of the entire plant	感病 Susceptible(S)
9	接种处24 h后黑褐色腐烂上下扩展,全株水渍状腐烂 Inoculation site showed black-brown rot expanding both upwards and downwards 24 h post- inoculation, resulting in water-soaked rot throughout the entire plant	高感 Highly susceptible (HS)

病情指数Disease index	抗性评价Resistance evaluation
DI≤5	抗病Resistant(R)
5<DI ≤ 20	中抗Moderately resistant(MR)
20<DI ≤ 50	中感Moderately susceptible(MS)
50<DI ≤ 100	感病Susceptible(S)

病情指数Disease index	抗性评价Resistance evaluation
DI≤5	抗病Resistant(R)
5<DI ≤ 20	中抗Moderately resistant(MR)
20<DI ≤ 50	中感Moderately susceptible(MS)
50<DI ≤ 100	感病Susceptible(S)

接种方法 Inoculation method	发病情况 Disease occurrence	方法优缺点 Advantages and disadvantages of the method
试管菌液插苗法 Tube inoculation method	发病显症快,全部发病 Rapid symptom development,all plants showing disease symptoms	发病率高过于严重,无法区分品种抗性 High disease incidence, unable to differentiate resistance levels among varieties
组培瓶蛭石菌液浸苗扦插法 Bacterial solution soaking and seedling cutting method in tissue culture bottles with vermiculite	显症快,发病严重 Rapid symptom development, severe disease incidence	发病率高,但过于严重,无法区分 High disease incidence, but too severe to differentiate
组培瓶蛭石薯苗针刺法 Acupuncture method of seedling in tissue culture bottles with vermiculite	发病严重 Severe disease incidence	发病严重但不稳定 Severe disease incidence but unstable
水泥槽蛭石拌菌移栽法 Cement trough with vermiculite inoculation and transplantation method	一星期后返青成活,零星发病 Recovery and sporadic disease occurrence after one week	发病率低,条件不易控制,不可行 Low disease incidence, difficult to control, not feasible
组培瓶蛭石扦插灌根法 Cutting and root irrigation method in tissue culture bottles with vermiculite	发病缓慢,零星发病 Slow disease development, sporadic disease occurrence	时间周期长且发病率低 Long time period and low disease incidence
盆栽土壤扦插接菌法 Potting soil inoculation method	一星期后返青成活,零星发病 Recovery and sporadic disease occurrence after one week.	发病率低,不可行 Low disease incidence, not feasible
组培瓶蛭石薯苗擦伤接菌法 Scratch inoculation method of seedlings in tissue culture bottles with vermiculite	显症与田间茎腐病相似,处理间发病较为一致 Similar symptoms to field stem rot, consistent disease progression, suitable disease incidence	病势进展适宜,发病率一致,条件易控制,是最可行的方法 Conditions are easily controlled, making it the most feasible method

甘薯抗茎腐病鉴定技术的建立及种质资源抗性分析

Establishment of sweet potato stem rot resistance identification technology and analysis of germplasm resource resistance

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编号 No.	品种/种质 Variety/Germplasm	病情指数 Disease index	编号 No.	品种/种质 Variety/Germplasm	病情指数 Disease index
1	YD7002	0.90	34	广薯87 Guangshu 87	27.90
2	DY7082-1	0.90	35	BN7006	27.90
3	浙紫薯1号Zhezi No.1	1.00	36	胜利百号Shengli	28.80
4	TF1118	1.80	37	浙薯77 Zheshu 77	31.50
5	DY7032	2.70	38	JF7020	32.40
6	南薯88 Nanshu 88	3.60	39	BN7051	32.40
7	浙紫薯4号Zhezi No.4	4.50	40	浙薯13 Zheshu 13	33.30
8	FM11	6.30	41	浙紫薯2号Zhezi No.2	33.30
9	渝薯17 Yushu 17	8.10	42	VP7002	33.30
10	FM42	8.10	43	浙薯259 Zheshu 259	34.20
11	浙薯38 Zheshu 38	9.00	44	心香Xinxiang	34.20
12	徐薯22 Xushu 22	11.70	45	徐薯18 Xushu 18	36.00
13	DF7006	12.60	46	浙773 Zhe 773	37.80
14	JK-1	13.50	47	CD4198	41.40
15	71847	14.40	48	DY7031	45.90
16	浙紫薯6号Zhezi No.6	18.00	49	C42	48.00
17	浙薯48 Zheshu 48	18.00	50	红牡丹Hongmudan	49.50
18	宁紫薯1号Ningzi No.1	18.90	51	DF7001	50.40
19	莫东Modong	18.90	52	浙薯132 Zheshu 132	51.30
20	浙紫薯5号Zhezi No.5	20.70	53	DY7159	54.00
21	浙薯2号Zheshu No.2	20.70	54	浙薯21 Zheshu 21	57.60
22	浙薯33 Zheshu 33	21.60	55	FM39	59.00
23	浙薯20 Zheshu 20	21.60	56	AY3089	59.40
24	DY7180	21.60	57	浙薯6025 Zheshu 6025	60.30
25	YD7001	23.40	58	BN7003	62.10
26	DY7207	24.30	59	NB7002	63.00
27	浙菜薯726 Zhecai 726	25.20	60	DY7109	64.80
28	4-17	25.20	61	六十工Liushigong	69.30
29	贵州红心Hongxin	26.10	62	DY7190	69.30
30	DW7028	26.10	63	DW7001-2	70.20
31	浙紫薯3号Zhezi No.3	27.00	64	YD7014	72.00
32	QZ 2-2	27.00	65	浙薯26 Zheshu 26	77.40
33	浙薯70 Zheshu 70	27.90

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