浙江农业学报 ›› 2024, Vol. 36 ›› Issue (9): 2110-2121.DOI: 10.3969/j.issn.1004-1524.20230816
水稻对林丹的吸收累积与代谢组学研究
展梦琪(
), 苏傲雪, 侯倩, 张皓宇, 姜欣蕊, 徐艳()
- 青岛大学 环境科学与工程学院,山东 青岛 266071
-
收稿日期:2023-06-29出版日期:2024-09-25发布日期:2024-09-30 -
作者简介:徐艳,E-mail:yanxu@qdu.edu.cn
展梦琪(1998—),女,山东泰安人,硕士研究生,主要研究方向为土壤微生物学。E-mail:zmq61626369@163.com -
通讯作者:徐艳,E-mail:yanxu@qdu.edu.cn -
基金资助:国家自然科学基金(42177028);山东省高等学校青创人才引育计划(DC2000000961)
Uptake and accumulation of lindane in rice and its metabolomics
ZHAN Mengqi(), SU Aoxue, HOU Qian, ZHANG Haoyu, JIANG Xinrui, XU Yan()
- School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China
-
Received:2023-06-29Online:2024-09-25Published:2024-09-30
摘要：
以有机氯代污染物——林丹作为研究对象,研究其在我国典型农作物——水稻(Oryza sativa L.)体内的迁移转化和代谢情况。结果发现,在林丹胁迫条件下,水稻根和地上部的林丹含量与水培溶液中的林丹浓度具有正相关性,表明水稻具有吸收林丹的能力。在不同林丹水培浓度下,水稻根内的林丹累积浓度在第二天均达到峰值,第三天后急剧下降。水稻根系内的林丹可以径向运输至茎叶中,但转运因子仅为0.08~0.13。在亚细胞水平上,水稻根中林丹的含量从高到低依次为细胞器>细胞壁>细胞质,表明细胞器和细胞壁是水稻根部林丹累积的主要部位。代谢组学分析结果表明,林丹胁迫下,脂质类代谢物的变化最显著,有43种上调、42种下调。受林丹胁迫影响,水稻根中的异牡荆苷等代谢物的含量提高,而天冬酰胺等代谢物的含量降低,多条植物代谢通路被改变,其中,鞘脂代谢通路和苯丙氨酸代谢通路受到较大影响。
中图分类号:
引用本文
展梦琪, 苏傲雪, 侯倩, 张皓宇, 姜欣蕊, 徐艳. 水稻对林丹的吸收累积与代谢组学研究[J]. 浙江农业学报, 2024, 36(9): 2110-2121.
ZHAN Mengqi, SU Aoxue, HOU Qian, ZHANG Haoyu, JIANG Xinrui, XU Yan. Uptake and accumulation of lindane in rice and its metabolomics[J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2110-2121.
图1 不同处理水稻根中林丹含量的动态变化
Fig.1 Dynamic of lindane content in rice root under treatments
图1 不同处理水稻根中林丹含量的动态变化
Fig.1 Dynamic of lindane content in rice root under treatments
图2 不同处理下水稻根部亚细胞水平(A)和茎叶(B)中的林丹含量及根中脂质含量(C)的变化 柱上无相同字母的表示差异显著(P<0.05)。
Fig.2 Changes of lindane content at rice subcellular level in root (A), lindane content in stem and leaf (B), and lipid content in root under treatments Bars marked without the same letters indicate significant difference at P<0.05.
图2 不同处理下水稻根部亚细胞水平(A)和茎叶(B)中的林丹含量及根中脂质含量(C)的变化 柱上无相同字母的表示差异显著(P<0.05)。
Fig.2 Changes of lindane content at rice subcellular level in root (A), lindane content in stem and leaf (B), and lipid content in root under treatments Bars marked without the same letters indicate significant difference at P<0.05.
图3 林丹胁迫下的水稻根系差异代谢物分布 FC为差异倍数,即处理组与对照组之比。下同。
Fig.3 Distribution of metabolites in rice root under lindane stress FC, Fold change, which is the ratio of the treatment grout and the control group.The same as below.
图3 林丹胁迫下的水稻根系差异代谢物分布 FC为差异倍数,即处理组与对照组之比。下同。
Fig.3 Distribution of metabolites in rice root under lindane stress FC, Fold change, which is the ratio of the treatment grout and the control group.The same as below.
图4 林丹胁迫下水稻根系差异代谢物的桑基图 Flavonoids,黄酮类化合物;Organonitrogen compounds,有机氮化合物;Isoflavonoids,异黄酮类化合物;Carboxylic acids and derivatives,羧酸及其衍生物;Fatty Acyls,脂肪酰基;Peptidomimetics,肽模拟物;Carbohydrates and carbohydrate conjugates,碳水化合物和碳水化合物结合物;Indoles and derivatives,吲哚及其衍生物;Prenol lipids,异戊二烯脂质;Phenylpropanoic acids,苯基丙酸;Steroids and steroid derivatives,类固醇和类固醇衍生物;Diazines,二氮杂苯;Purine nucleosides,嘌呤核苷;Endogenous metabolites,内源性代谢物;Azacyclic compounds,氮杂环状化合物;Flavonoid glycosides,黄酮苷;Amines,胺;Isoflavonoid O-glycosides,异黄酮O-糖苷;Flavones,黄酮; Amino acids, peptides, and analogues,氨基酸、肽和类似物;Fatty acids and conjugates,脂肪酸和缀合物;Hybrid peptides,杂交肽;Tricarboxylic acids and derivatives,三羧酸及其衍生物;Glycosyl compounds,糖基化合物;Indoles,吲哚;Eicosanoids,类花生酸类化合物;Diterpenoids,二萜;Sesquiterpenoids,倍半萜类化合物;Androstane steroids,雄甾烷类固醇;Pyrimidines and pyrimidine derivatives,嘧啶及其衍生物;Lineolic acids and derivatives,线性酸及其偶联物;Alcohols and polyols,醇类和多元醇;NULL,未分类。
Fig.4 Sangi diagram of differential metabolites in rice root under lindane stress
图4 林丹胁迫下水稻根系差异代谢物的桑基图 Flavonoids,黄酮类化合物;Organonitrogen compounds,有机氮化合物;Isoflavonoids,异黄酮类化合物;Carboxylic acids and derivatives,羧酸及其衍生物;Fatty Acyls,脂肪酰基;Peptidomimetics,肽模拟物;Carbohydrates and carbohydrate conjugates,碳水化合物和碳水化合物结合物;Indoles and derivatives,吲哚及其衍生物;Prenol lipids,异戊二烯脂质;Phenylpropanoic acids,苯基丙酸;Steroids and steroid derivatives,类固醇和类固醇衍生物;Diazines,二氮杂苯;Purine nucleosides,嘌呤核苷;Endogenous metabolites,内源性代谢物;Azacyclic compounds,氮杂环状化合物;Flavonoid glycosides,黄酮苷;Amines,胺;Isoflavonoid O-glycosides,异黄酮O-糖苷;Flavones,黄酮; Amino acids, peptides, and analogues,氨基酸、肽和类似物;Fatty acids and conjugates,脂肪酸和缀合物;Hybrid peptides,杂交肽;Tricarboxylic acids and derivatives,三羧酸及其衍生物;Glycosyl compounds,糖基化合物;Indoles,吲哚;Eicosanoids,类花生酸类化合物;Diterpenoids,二萜;Sesquiterpenoids,倍半萜类化合物;Androstane steroids,雄甾烷类固醇;Pyrimidines and pyrimidine derivatives,嘧啶及其衍生物;Lineolic acids and derivatives,线性酸及其偶联物;Alcohols and polyols,醇类和多元醇;NULL,未分类。
Fig.4 Sangi diagram of differential metabolites in rice root under lindane stress
图5 林丹胁迫下水稻体内丰度上调(A)和下调(B)效果显著的前10种代谢物 Isovitexin,异牡荆苷;Orientin,荭草苷;Astragalin,黄芪苷;Luteolin 7-glucoside,木犀草苷;Sphinganine,二氢鞘氨醇;Isobutyl 4-hydroxybenzoate,尼泊金异丁酯;Genistin,染料木苷;Spermine,精胺;Apigenin,芹菜素;Leucylproline,亮氨酰脯氨酸;L-Asparagine,天冬酰胺;Adenosine,腺苷;Azelaic acid,壬二酸;51-45-6,组胺;Diethanolamine,二乙醇胺;Jasmonic acid,茉莉酸;Diosgenin,皂素;Vidarabine,阿糖腺苷;2-Chloro-L-phenylalanine,2-氯-L-苯丙氨酸;5-Dehydroadenosine,嘌呤核苷类似物。
Fig.5 The top ten metabolites with significant up-regulation (A) and down-regulation (B) of abundance in rice root under lindane stress
图5 林丹胁迫下水稻体内丰度上调(A)和下调(B)效果显著的前10种代谢物 Isovitexin,异牡荆苷;Orientin,荭草苷;Astragalin,黄芪苷;Luteolin 7-glucoside,木犀草苷;Sphinganine,二氢鞘氨醇;Isobutyl 4-hydroxybenzoate,尼泊金异丁酯;Genistin,染料木苷;Spermine,精胺;Apigenin,芹菜素;Leucylproline,亮氨酰脯氨酸;L-Asparagine,天冬酰胺;Adenosine,腺苷;Azelaic acid,壬二酸;51-45-6,组胺;Diethanolamine,二乙醇胺;Jasmonic acid,茉莉酸;Diosgenin,皂素;Vidarabine,阿糖腺苷;2-Chloro-L-phenylalanine,2-氯-L-苯丙氨酸;5-Dehydroadenosine,嘌呤核苷类似物。
Fig.5 The top ten metabolites with significant up-regulation (A) and down-regulation (B) of abundance in rice root under lindane stress
图6 林丹胁迫下水稻代谢通路的变化 Sphingolipid metabolism,鞘脂代谢;Phenylalanine metabolism,苯丙氨酸代谢;beta-Alanine metabolism,β-丙氨酸代谢;Glyoxylate and dicarboxylate metabolism,乙醛酸和二羧酸代谢;TCA cycle,TCA循环;Pantothenate and CoA biosynthesis,泛酸和辅酶A生物合成;Alanine aspartate and glutamate metabolism,丙氨酸天冬氨酸与谷氨酸代谢;Histidine metabolism,组氨酸代谢;Butanoate metabolism,丁醇代谢;Arginine biosynthesis,精氨酸生物合成;Taurine and hypotaurine metabolism,牛磺酸与低牛磺酸代谢;Vitamin B6 metabolism,维生素B6代谢;Pyrimidine metabolism,嘧啶代谢;Palmitoyl-CoA,棕榈酰辅酶A;3-Dehydrosphinganine,3-脱氢鞘氨醇;Sphinganine,鞘氨醇;Dihydroceramide,二氢神经酰胺;N-Acylsphingosine,N-酰基鞘氨醇;Psychosine,鞘氨醇半乳糖苷;Sphingosine 1-phosphate,1-磷酸鞘氨醇;Ethanolamine phosphate,乙醇胺磷酸酯。 每一个圆圈代表一种代谢通路,圆圈越大,相关性越强,颜色越红,受影响程度越大。
Fig.6 Changes of rice metabolic pathway under lindane stress Each circle represents a metabolic pathway, and the larger the circle, the stronger the correlation, and the redder the color, the bigger influence.
图6 林丹胁迫下水稻代谢通路的变化 Sphingolipid metabolism,鞘脂代谢;Phenylalanine metabolism,苯丙氨酸代谢;beta-Alanine metabolism,β-丙氨酸代谢;Glyoxylate and dicarboxylate metabolism,乙醛酸和二羧酸代谢;TCA cycle,TCA循环;Pantothenate and CoA biosynthesis,泛酸和辅酶A生物合成;Alanine aspartate and glutamate metabolism,丙氨酸天冬氨酸与谷氨酸代谢;Histidine metabolism,组氨酸代谢;Butanoate metabolism,丁醇代谢;Arginine biosynthesis,精氨酸生物合成;Taurine and hypotaurine metabolism,牛磺酸与低牛磺酸代谢;Vitamin B6 metabolism,维生素B6代谢;Pyrimidine metabolism,嘧啶代谢;Palmitoyl-CoA,棕榈酰辅酶A;3-Dehydrosphinganine,3-脱氢鞘氨醇;Sphinganine,鞘氨醇;Dihydroceramide,二氢神经酰胺;N-Acylsphingosine,N-酰基鞘氨醇;Psychosine,鞘氨醇半乳糖苷;Sphingosine 1-phosphate,1-磷酸鞘氨醇;Ethanolamine phosphate,乙醇胺磷酸酯。 每一个圆圈代表一种代谢通路,圆圈越大,相关性越强,颜色越红,受影响程度越大。
Fig.6 Changes of rice metabolic pathway under lindane stress Each circle represents a metabolic pathway, and the larger the circle, the stronger the correlation, and the redder the color, the bigger influence.
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