Bioinformatic analysis, subcellular lcalization, and expression analysis of SlTCP17 gene

doi:10.3969/j.issn.1004-1524.20250715

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (6): 1218-1227.DOI: 10.3969/j.issn.1004-1524.20250715

• Horticultural Science • Previous Articles Next Articles

Bioinformatic analysis, subcellular lcalization, and expression analysis of SlTCP17 gene

LOU Shihao(), XU Jinxin, JI Zelin, WANG Tao, NIU Xiaohui, DENG Jiaying, ZHANG Tao, GUO Zhixin, PIAO Fengzhi, DONG Han^*()

College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China

Received:2025-11-21 Online:2026-06-25 Published:2026-07-14

Abstract

Abstract:

TCP transcription factor is the core regulatory element to regulate plant growth and development and hormone signal transduction process. In order to discover the TCP family members in tomato (Solanum lycopersicum L.) who respond to adversity stress, a TCP family gene SlTCP17 was cloned in this study. We systematically analyzed it by bioinformatics, subcellular localization and qRT-PCR technology. The results showed that SlTCP17 was located in the nucleus, encoding an unstable hydrophilic protein with a relative molecular weight of 45.26 ku, and its secondary structure was mainly random coil (62.74%). Phylogenetic analysis showed that SlTCP17 and SlTCP23 have the highest affinity, followed by AtTCP8. The promoter region was rich in a variety of Cis-acting element related to stress, hormones and light response. The gene showed tissue specificity of high expression in leaves and low expression in roots, and its transcription level was induced by drought, but inhibited by salt and Phytophthora capsici infection. These findings indicate that SlTCP17 is widely involved in the regulation of tomato stress response, which lays a foundation for explaining its molecular mechanism.

Key words: TCP transcription factor, tomato, adversity stress, bioinformatics analysis, subcellular localization

CLC Number:

S685.12

LOU Shihao, XU Jinxin, JI Zelin, WANG Tao, NIU Xiaohui, DENG Jiaying, ZHANG Tao, GUO Zhixin, PIAO Fengzhi, DONG Han. Bioinformatic analysis, subcellular lcalization, and expression analysis of SlTCP17 gene[J]. Acta Agriculturae Zhejiangensis, 2026, 38(6): 1218-1227.

Figures/Tables 11

References 36

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引物名称 Primer name	引物序列(5'→3') Primer sequence(5'→3')	注释 Annotation
RT-SlTCP17-F	AGTTATAGCTGCTACGGG	qRT-PCR
RT-SlTCP17-R	TTGAGATGGTACGGACAT
RT-ACTIN2-F	TGTCCCTATTTACGAGGGTTATGC
RT-ACTIN2-R	CAGTTAAATCACGACCAGCAAGAT
RT-UBI3-F	GCCGACTACAACATCCAGAAGG
RT-UBI3-R	TGCAACACAGCGAGCTTAACC
GFP-SlTCP17-F	ctctcgagctttcgc gagctcATGGAAGGAGGAGGAGGTGACG	融合绿色荧光蛋白Green fluorescent protein
GFP-SlTCP17-R	gcccttgctcaccat ggatccGGAATTATGATGGCTTGTTGAATCA

引物名称 Primer name	引物序列(5'→3') Primer sequence(5'→3')	注释 Annotation
RT-SlTCP17-F	AGTTATAGCTGCTACGGG	qRT-PCR
RT-SlTCP17-R	TTGAGATGGTACGGACAT
RT-ACTIN2-F	TGTCCCTATTTACGAGGGTTATGC
RT-ACTIN2-R	CAGTTAAATCACGACCAGCAAGAT
RT-UBI3-F	GCCGACTACAACATCCAGAAGG
RT-UBI3-R	TGCAACACAGCGAGCTTAACC
GFP-SlTCP17-F	ctctcgagctttcgc gagctcATGGAAGGAGGAGGAGGTGACG	融合绿色荧光蛋白Green fluorescent protein
GFP-SlTCP17-R	gcccttgctcaccat ggatccGGAATTATGATGGCTTGTTGAATCA

名称 Name	序列 Sequence	功能 Function	数量 Number
TATA-box	ATATAA	核心启动子元件在转录开始的-30左右 Core promoter element around -30 of transcription start	4
	ATATAT		7
	ATTATA		4
	TACATAAA		1
	TATA		21
	TATAA		4
	TATACA		2
	TATATA		11
	TATATAA		2
	TATTTAAA		1
ABRE	TACGGTC	参与脱落酸反应的顺式作用元件 Cis-acting element involved in the abscisic acid responsiveness	1
	CACGTG		1
	ACGTG		1
ARE	AAACCA	对厌氧诱导必不可少的顺式作用元件 Cis-acting element essential for the anaerobic induction	1
Box 4	ATTAAT	与光反应有关的保守DNA模块的一部分 Part of a conserved DNA module involved in light responsiveness	4
CAAT-box	CAAAT	启动子和增强子区域中常见的顺式作用元件 Common cis-acting element in promoter and enhancer regions	11
	CCAAT		3
CCAAT-box	CAACGG	MYBHv1结合位点MYBHv1 binding site	1
CGTCA-motif	CGTCA	参与MeJA反应的顺式作用元件Cis-acting element involved in the MeJA-responsiveness	3
TGACG-motif	TGACG	光响应元件的一部分Part of a light responsive element	3
chs-CMA1a	TTACTTAA		1
GA-motif	ATAGATAA		1
GATA-motif	GATAGGA		1
TCT-motif	TCTTAC		1
GT1-motif	CACGTG		2
	GGTTAA		1
G-Box	CACGAC	参与光响应的顺式作用元件Cis-acting element involved in light responsiveness	1
	CACGTG		2
HD-Zip 3	GTAAT(G/C)ATTAC	蛋白质结合位点Protein binding site	1
MRE	AACCTAA	MYB结合位点参与光响应MYB binding site involved in light responsiveness	2
P-box	CCTTTTG	赤霉素响应元件Gibberellin-responsive element	2
TGA-element	AACGAC	生长素响应元件Auxin-responsive element	2

名称 Name	序列 Sequence	功能 Function	数量 Number
TATA-box	ATATAA	核心启动子元件在转录开始的-30左右 Core promoter element around -30 of transcription start	4
	ATATAT		7
	ATTATA		4
	TACATAAA		1
	TATA		21
	TATAA		4
	TATACA		2
	TATATA		11
	TATATAA		2
	TATTTAAA		1
ABRE	TACGGTC	参与脱落酸反应的顺式作用元件 Cis-acting element involved in the abscisic acid responsiveness	1
	CACGTG		1
	ACGTG		1
ARE	AAACCA	对厌氧诱导必不可少的顺式作用元件 Cis-acting element essential for the anaerobic induction	1
Box 4	ATTAAT	与光反应有关的保守DNA模块的一部分 Part of a conserved DNA module involved in light responsiveness	4
CAAT-box	CAAAT	启动子和增强子区域中常见的顺式作用元件 Common cis-acting element in promoter and enhancer regions	11
	CCAAT		3
CCAAT-box	CAACGG	MYBHv1结合位点MYBHv1 binding site	1
CGTCA-motif	CGTCA	参与MeJA反应的顺式作用元件Cis-acting element involved in the MeJA-responsiveness	3
TGACG-motif	TGACG	光响应元件的一部分Part of a light responsive element	3
chs-CMA1a	TTACTTAA		1
GA-motif	ATAGATAA		1
GATA-motif	GATAGGA		1
TCT-motif	TCTTAC		1
GT1-motif	CACGTG		2
	GGTTAA		1
G-Box	CACGAC	参与光响应的顺式作用元件Cis-acting element involved in light responsiveness	1
	CACGTG		2
HD-Zip 3	GTAAT(G/C)ATTAC	蛋白质结合位点Protein binding site	1
MRE	AACCTAA	MYB结合位点参与光响应MYB binding site involved in light responsiveness	2
P-box	CCTTTTG	赤霉素响应元件Gibberellin-responsive element	2
TGA-element	AACGAC	生长素响应元件Auxin-responsive element	2

Bioinformatic analysis, subcellular lcalization, and expression analysis of SlTCP17 gene

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