Genetic diversity of Apis cerana in Zhejiang, China based on mitochondrial DNA tRNAleu-COⅡ sequence

doi:10.3969/j.issn.1004-1524.2022.11.09

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (11): 2395-2403.DOI: 10.3969/j.issn.1004-1524.2022.11.09

• Animal Science • Previous Articles Next Articles

Genetic diversity of Apis cerana in Zhejiang, China based on mitochondrial DNA tRNA^leu-COⅡ sequence

CAO Lianfei¹(), SHI Jinhu², XU Yalan³, SU Xiaoling⁴, HU Fuliang³, ZHENG Huoqing³^,^*()

1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Zhejiang Animal Husbandry Technology Promotion and Breeding Livestock and Poultry Monitoring Station, Hangzhou 310021, China
3. College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
4. Jinhua Academy of Agricultural Science, Jinhua 321000, Zhejiang, China

Received:2022-02-15 Online:2022-11-25 Published:2022-11-29
Contact: ZHENG Huoqing

Abstract

Abstract:

To further understand the genetic diversity of Apis cerana in Zhejiang Province, the genetic variation and population genetic structure were determined based on the mitochondrial DNA tRNA^leu-COⅡ sequences of 448 samples from ten prefecture-level cities in Zhejiang, China. Forty-six haplotypes were found and the haplotype diversity (H_d) was 0.665 7, the nucleotide diversity (P_i) was 0.002 61, which indicated that A. cerana in Zhejiang Province had relatively high haplotype diversity and low nucleotide diversity. Neutral test and network of haplotypes showed that A. cerana in Zhejiang Province might have experienced recent expansion. Population genetic structure analysis showed that A. cerana populations in Hangzhou and Ningbo had a certain extent of genetic differentiations with other surrounding A. cerana populations, which was possibly related to the survivorship and adaptation of A. cerana in the local areas over a long period of time and a certain geographical isolation. A new haplotype that highly differentiated with other haplotypes of A. cerana in China was found in this study.

Key words: Zhejiang, Apis cerana, genetic diversity, mitochondrial DNA

CLC Number:

S891

CAO Lianfei, SHI Jinhu, XU Yalan, SU Xiaoling, HU Fuliang, ZHENG Huoqing. Genetic diversity of Apis cerana in Zhejiang, China based on mitochondrial DNA tRNA^leu-COⅡ sequence[J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2395-2403.

Figures/Tables 6

References 29

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地级市 Prefecture- level city	县级行政区 County	样本数 Number of samples	地级市 Prefecture- level city	县级行政区 County	样本数 Number of samples	地级市 Prefecture- level city	县级行政区 County	样本数 Number of samples
杭州Hangzhou	临安Lin’an	19	丽水Lishui	缙云Jinyun	11	温州Wenzhou	文成Wencheng	30
	桐庐Tonglu	15		莲都Liandu	16		泰顺Taishun	21
	淳安Chun’an	17		松阳Songyang	15		苍南Cangnan	5
金华Jinhua	婺城Wucheng	9		云和Yunhe	5		乐清Yueqing	5
	金东Jindong	9		遂昌Suichang	6	宁波Ningbo	北仑Beilun	15
	兰溪Lanxi	9		龙泉Longquan	15		象山Xiangshan	17
	义乌Yiwu	9		青田Qingtian	10		慈溪Cixi	5
	东阳Dongyang	9		景宁Jingning	6		奉化Fenghua	5
	永康Yongkang	9		庆元Qingyuan	10	绍兴Shaoxing	新昌Xinchang	15
	武义Wuyi	9	衢州Quzhou	开化Kaihua	15		嵊州Shengzhou	4
	浦江Pujiang	9		龙游Longyou	15		柯桥Keqiao	5
	磐安Pan’an	9		衢江Qujiang	10		上虞Shangyu	5
台州Taizhou	天台Tiantai	15		江山Jiangshan	6	舟山Zhoushan	定海Dinghai	4
	三门Sanmen	10	湖州Huzhou	长兴Changxing	5
	黄岩Huangyan	5		安吉Anji	5

地级市 Prefecture- level city	县级行政区 County	样本数 Number of samples	地级市 Prefecture- level city	县级行政区 County	样本数 Number of samples	地级市 Prefecture- level city	县级行政区 County	样本数 Number of samples
杭州Hangzhou	临安Lin’an	19	丽水Lishui	缙云Jinyun	11	温州Wenzhou	文成Wencheng	30
	桐庐Tonglu	15		莲都Liandu	16		泰顺Taishun	21
	淳安Chun’an	17		松阳Songyang	15		苍南Cangnan	5
金华Jinhua	婺城Wucheng	9		云和Yunhe	5		乐清Yueqing	5
	金东Jindong	9		遂昌Suichang	6	宁波Ningbo	北仑Beilun	15
	兰溪Lanxi	9		龙泉Longquan	15		象山Xiangshan	17
	义乌Yiwu	9		青田Qingtian	10		慈溪Cixi	5
	东阳Dongyang	9		景宁Jingning	6		奉化Fenghua	5
	永康Yongkang	9		庆元Qingyuan	10	绍兴Shaoxing	新昌Xinchang	15
	武义Wuyi	9	衢州Quzhou	开化Kaihua	15		嵊州Shengzhou	4
	浦江Pujiang	9		龙游Longyou	15		柯桥Keqiao	5
	磐安Pan’an	9		衢江Qujiang	10		上虞Shangyu	5
台州Taizhou	天台Tiantai	15		江山Jiangshan	6	舟山Zhoushan	定海Dinghai	4
	三门Sanmen	10	湖州Huzhou	长兴Changxing	5
	黄岩Huangyan	5		安吉Anji	5

地理群体 Population	群体代码 Population code	样本数 Number of samples	单倍型数 Number of haplotypes	特有单倍型数 Number of unique haplotypes	单倍型多样度 Haplotype diversity (H_d)	核苷酸多样度 Nucleotide diversity (P_i)	核苷酸平均差异数 Average number of nucleotide differences(K)	Tajima’s D 中性检验 Tajima’s D neutrality test	Fu’s F_s 中性检验 Fu’s F_s neutrality test
杭州Hangzhou	HZ	51	8	4	0.625 9	0.002 24	0.924	-1.500 73^*	-3.129 81^*
金华Jinhua	JH	81	14	5	0.610 2	0.001 62	0.671	-2.066 40^**	-11.378 70^**
丽水Lishui	LS	94	20	9	0.772 0	0.003 52	1.452	-1.528 29^*	-13.140 55^**
衢州Quzhou	QZ	46	5	0	0.632 9	0.002 14	0.888	-0.541 53	-0.516 34
温州Wenzhou	WZ	61	8	2	0.510 9	0.003 85	1.595	-1.940 05^**	-0.938 06
台州Taizhou	TZ	30	8	2	0.650 6	0.001 77	0.731	-1.732 20^*	-4.653 73^**
宁波Ningbo	NB	42	10	2	0.629 5	0.001 73	0.718	-1.733 64^*	-7.152 31^**
绍兴Shaoxing	SX	29	9	3	0.482 8	0.001 81	0.751	-2.105 19^**	-5.313 74^**
湖州Huzhou	HUZ	10	7	2	0.866 7	0.002 42	1.000	-0.541 53	-0.516 34
舟山Zhoushan	ZS	4	4	0	1.000 0	0.003 62	1.500	-0.754 45	-2.367 12^*
全部All	ALL	448	46	29	0.665 7	0.002 61	1.076	-2.306 99^**	-28.041 79^**

地理群体 Population	群体代码 Population code	样本数 Number of samples	单倍型数 Number of haplotypes	特有单倍型数 Number of unique haplotypes	单倍型多样度 Haplotype diversity (H_d)	核苷酸多样度 Nucleotide diversity (P_i)	核苷酸平均差异数 Average number of nucleotide differences(K)	Tajima’s D 中性检验 Tajima’s D neutrality test	Fu’s F_s 中性检验 Fu’s F_s neutrality test
杭州Hangzhou	HZ	51	8	4	0.625 9	0.002 24	0.924	-1.500 73^*	-3.129 81^*
金华Jinhua	JH	81	14	5	0.610 2	0.001 62	0.671	-2.066 40^**	-11.378 70^**
丽水Lishui	LS	94	20	9	0.772 0	0.003 52	1.452	-1.528 29^*	-13.140 55^**
衢州Quzhou	QZ	46	5	0	0.632 9	0.002 14	0.888	-0.541 53	-0.516 34
温州Wenzhou	WZ	61	8	2	0.510 9	0.003 85	1.595	-1.940 05^**	-0.938 06
台州Taizhou	TZ	30	8	2	0.650 6	0.001 77	0.731	-1.732 20^*	-4.653 73^**
宁波Ningbo	NB	42	10	2	0.629 5	0.001 73	0.718	-1.733 64^*	-7.152 31^**
绍兴Shaoxing	SX	29	9	3	0.482 8	0.001 81	0.751	-2.105 19^**	-5.313 74^**
湖州Huzhou	HUZ	10	7	2	0.866 7	0.002 42	1.000	-0.541 53	-0.516 34
舟山Zhoushan	ZS	4	4	0	1.000 0	0.003 62	1.500	-0.754 45	-2.367 12^*
全部All	ALL	448	46	29	0.665 7	0.002 61	1.076	-2.306 99^**	-28.041 79^**

群体代码 Population code	HZ	JH	LS	QZ	WZ	TZ	NB	SX	HUZ	ZS
HZ		0.002 10	0.003 13	0.002 45	0.003 27	0.001 99	0.002 08	0.002 14	0.002 34	0.003 05
JH	0.076 98^**		0.002 75	0.002 03	0.002 87	0.001 79	0.001 73	0.001 75	0.001 99	0.002 55
LS	0.037 66^**	0.032 03^**		0.002 89	0.003 82	0.002 85	0.002 73	0.002 79	0.003 14	0.003 56
QZ	0.072 10^**	0.058 28^**	0.033 49^*		0.003 11	0.002 15	0.002 00	0.002 07	0.002 42	0.002 63
WZ	0.042 00^*	0.022 06^*	-0.004 78	0.032 42^*		0.002 97	0.002 85	0.002 91	0.003 26	0.003 67
TZ	0.034 45	0.036 80	0.040 09	0.046 85	-0.003 95		0.001 79	0.001 84	0.002 08	0.002 75
NB	0.099 91^**	0.071 92^**	0.025 31^*	0.022 09^*	0.034 51^*	0.081 59^*		0.001 77	0.002 09	0.002 54
SX	-0.009 72	0.038 63^**	0.019 11	0.052 52^**	0.018 33	0.026 05	0.083 23^**		0.002 12	0.002 65
HUZ	0.051 17^**	0.036 38^**	0.000 74	0.027 11^**	0.003 98	0.024 64	0.018 62	0.029 28		0.003 03
ZS	0.100 38	0.057 78	0.020 72	-0.007 38	0.019 93	-0.014 33	-0.040 08	0.090 60	-0.040 97

Genetic diversity of Apis cerana in Zhejiang, China based on mitochondrial DNA tRNA^leu-COⅡ sequence

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群体分组 Group	组间变异 Variation among groups/%	组内群体间变异 Variation among populations within group/%	群体内变异 Variation within population/%	F_ct	F_sc	F_st
10个地级市	1.25	11.16	87.59	0.012 48	0.113 02^**	0.124 09^**
Ten prefecture-level cities
5个自然地理区	1.02	11.45	87.53	0.010 22^*	0.115 70^**	0.124 74^**
Five geographic regions

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