Optimization of low temperature resistant corn stalk degrading bacterial community and its effect

doi:10.3969/j.issn.1004-1524.2022.12.15

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (12): 2720-2727.DOI: 10.3969/j.issn.1004-1524.2022.12.15

• Environmental Science • Previous Articles Next Articles

Optimization of low temperature resistant corn stalk degrading bacterial community and its effect

WANG Yiran¹^,²(), KANG Zhichao¹, ZHU Guopeng¹, WANG Yang³, QI Geqi³^,^*(), YU Hongwen¹^,^*()

1. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China
2. School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun 130022, China
3. Hinggan League Institute of Agricultural and Animal Husbandry Sciences, Agriculture and Animal Husbandry Bureau of Hinggan League, Hinggan 137400, Inner Mongolia, China

Received:2022-03-22 Online:2022-12-25 Published:2022-12-26
Contact: QI Geqi,YU Hongwen

Abstract

Abstract:

In order to adapt to the climate characteristics of low average annual temperature and frequent freezing and thawing in northeast China, this study optimized the construction of microbial community which could efficiently degrade straw in low temperature environment. The metagenomic sequencing technology was employed for correlation analysis of six bacterial genera (Sphingobacter, Arthrobacter, Flavobacterium, Geobacter, Delfteia and Sphingosphinomonas) in JZ5, an original frigostable straw degrading bacterial community. Besides, the composition of the original community JZ5 was simplified. The results showed that after 12 d of culture at 15 ℃, pH value of optimized community CJZ1 and CJZ2 increased by 0.76 and 0.66 compared with the initial value, respectively, and the degradation rate of straw increased by 0.47 and 0.25 percentage point compared with the original community, respectively, with no significant difference. In addition, the active peak of laccase and lignin peroxidase significantly decreased, while cellulase activity increased by 43.66% and 37.99%, hemicellulase activity increase by 21.40% and 12.91%, respectively in CJZ1 and CJZ2. The results could provide a new idea for the optimization of bacterial community, and a high quality bacterial community for the efficient degradation of straw at low temperature.

Key words: low temperature, straw, bacterial community, degradation rate, Lignin peroxidase, cellulase, hemicellulase

CLC Number:

S141.4
X712

WANG Yiran, KANG Zhichao, ZHU Guopeng, WANG Yang, QI Geqi, YU Hongwen. Optimization of low temperature resistant corn stalk degrading bacterial community and its effect[J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2720-2727.

Figures/Tables 7

Table 1 Bacteria composition

分组 Group	不同属的菌株数量Number of strains from different genera
分组 Group	鞘氨醇杆菌 Sphingobacter	代尔夫特菌 Delfteia	鞘氨醇单胞菌 Sphingosphinomonas	地杆菌 Geobacter	黄杆菌 Flavobacterium	节杆菌 Arthrobacter
JZ5	10	1	1	4	2	2
CJZ1	10	1	1	4	2	0
CJZ2	10	1	1	4	0	0

Fig.1 pH of different bacterial communities

Fig.2 Laccase activity of different bacterial communities

Fig.3 Lignin peroxidase activity of different bacterial communities

Fig.4 Cellulase activity curve of different bacterial communities

Fig.5 Hemicellulase activity of different bacterial communities

Fig.6 Degradation rate of straw by different bacterial communities

References 31

[1]	中华人民共和国国家统计局. 中国统计年鉴[M]. 北京: 中国统计出版社, 2020.
[2]	刘海燕, 孙善文, 韩业辉, 等. 黑龙江省玉米秸秆还田现状及发展策略[J]. 中国种业, 2021(11): 20-22.
	LIU H Y, SUN S W, HAN Y H, et al. The current situation and development strategy of corn straw returning in Heilongjiang Province[J]. China Seed Industry, 2021(11): 20-22. (in Chinese)
[3]	张必周. 玉米秸秆低温高效降解复合菌GF-20宏基因组学解析及单菌株分离与复配[D]. 呼和浩特: 内蒙古农业大学, 2020.
	ZHANG B Z. Metagenomic analysis of GF-20 with efficient decomposition of corn stover at low temperature and isolation and construction of single strains[D]. Hohhot: Inner Mongolia Agricultural University, 2020. (in Chinese with English abstract)
[4]	BILAL M, ASGHER M, IQBAL H M N, et al. Bio-based degradation of emerging endocrine-disrupting and dye-based pollutants using cross-linked enzyme aggregates[J]. Environmental Science and Pollution Research, 2017, 24(8): 7035-7041. DOI URL
[5]	穆春雷, 武晓森, 李术娜, 等. 低温产纤维素酶菌株的筛选、鉴定及纤维素酶学性质[J]. 微生物学通报, 2013, 40(7): 1193-1201.
	MU C L, WU X S, LI S N, et al. Screening and identification of a cold-adapted cellulase-producing strains and characterization of cellulase[J]. Microbiology China, 2013, 40(7): 1193-1201. (in Chinese with English abstract)
[6]	张晓波. 东北平原地区玉米秸秆还田模式的探讨[J]. 农业开发与装备, 2017(9): 53.
	ZHANG X B. Discussion on returning maize straw to field in northeast Plain[J]. Agricultural Development & Equipments, 2017(9): 53. (in Chinese)
[7]	王新光, 田磊, 王恩泽, 等. 玉米秸秆高效降解微生物复合菌系的构建及降解效果评价[J]. 生物技术通报, 2022, 38(4): 217-229.
	WANG X G, TIAN L, WANG E Z, et al. Construction of microbial consortium for efficient degradation of corn straw and evaluation of its degradation effect[J]. Biotechnology Bulletin, 2022, 38(4): 217-229. (in Chinese with English abstract)
[8]	LIANG J J, FANG X X, LIN Y Q, et al. A new screened microbial consortium OEM2 for lignocellulosic biomass deconstruction and chlorophenols detoxification[J]. Journal of Hazardous Materials, 2018, 347: 341-348. DOI PMID
[9]	苏鑫. 常温细菌复合菌系降解木质素的协同作用解析[D]. 大庆: 黑龙江八一农垦大学, 2020.
	SU X. Synergistic analysis of lignin degradation by normal temperature bacteria complex strains[D]. Daqing: Heilongjiang Bayi Agricultural University, 2020. (in Chinese with English abstract)
[10]	张必周, 青格尔, 高聚林, 等. 低温复配菌系对玉米秸秆的降解特性及稳定性[J]. 生态学杂志, 2022, 41(3): 487-494.
	ZHANG B Z, QING G E, GAO J L, et al. Degradation characteristics and adaptability of corn stover by composite microbial system at low temperature[J]. Chinese Journal of Ecology, 2022, 41(3): 487-494. (in Chinese with English abstract)
[11]	杨梦雅, 闫非凡, 闫美超, 等. 低温木质纤维素分解复合菌系PLC-8对玉米秸秆的分解特性[J]. 中国农业科技导报, 2021, 23(1): 73-81. DOI
	YANG M Y, YAN F F, YAN M C, et al. Decomposition characteristics of corn stover by microbial consortium PLC-8 with lignocellulose-degradation at low temperature[J]. Journal of Agricultural Science and Technology, 2021, 23(1): 73-81. (in Chinese with English abstract)
[12]	BODDY L. Interspecific combative interactions between wood-decaying basidiomycetes[J]. FEMS Microbiology Ecology, 2000, 31(3): 185-194. PMID
[13]	李文明, 李伟英, 陆辉, 等. 供水管网水样R2A培养基细菌总数测定及其影响因素探究[J]. 净水技术, 2014, 33(6): 66-70.
	LI W M, LI W Y, LU H, et al. Influencing factors and determination of total bacterial count of water samples on R2A medium in water supply distribution system[J]. Water Purification Technology, 2014, 33(6): 66-70. (in Chinese with English abstract)
[14]	王强. 降解木质纤维素的侧耳属菌株的筛选及酶学特性研究[D]. 邯郸: 河北工程大学, 2019.
	WANG Q. Screening and enzymatic characterization of Pleurotus strains degrading lignocellulose[D]. Handan: Hebei University of Engineering, 2019. (in Chinese with English abstract)
[15]	燕红, 苏俊, 于彩莲, 等. 高效木质素降解菌株的分离筛选[J]. 浙江大学学报(农业与生命科学版), 2011, 37(3): 259-262.
	YAN H, SU J, YU C L, et al. Isolation and screening of fungal strains with high ligninolytic enzyme activities[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2011, 37(3): 259-262. (in Chinese with English abstract)
[16]	薛藩. 纤维素降解微生物的筛选及高效纤维素酶活条件研究[D]. 扬州: 扬州大学, 2019.
	XUE F. Screening of cellulose-degrading microorganisms and research on conditions of efficient cellulase activity[D]. Yangzhou: Yangzhou University, 2019. (in Chinese with English abstract)
[17]	杨青, 汪斌, 王亚伟, 等. 介导两种半纤维素酶分泌表达的信号肽比较[J]. 中国生物工程杂志, 2017, 37(8): 15-22.
	YANG Q, WANG B, WANG Y W, et al. Comparison of signal peptides for two hemicellulase secretory expression[J]. China Biotechnology, 2017, 37(8): 15-22. (in Chinese with English abstract)
[18]	杨梦雅. 低温高效复合菌系PLC-8对木质纤维素分解特性及菌株协同效应初探[D]. 延吉: 延边大学, 2020.
	YANG M Y. The characteristic degradation of lignocellulose and primary synergism effect of strains by low temperature and high efficiency microbial consortium PLC-8[D]. Yanji: Yanbian University, 2020. (in Chinese with English abstract)
[19]	MALHERBE S, CLOETE T E. Lignocellulose biodegradation: fundamentals and applications[J]. Reviews in Environmental Science and Bio, 2002, 1(2): 105-114.
[20]	周静, 黄文茂, 秦利军, 等. 四株PGPR菌株混菌发酵体系的构建及促生效应评价[J]. 生物技术通报, 2021, 37(4): 116-126. DOI
	ZHOU J, HUANG W M, QIN L J, et al. Construction of mixed fermentation system of four PGPR strains and evaluation of its promoting effect[J]. Biotechnology Bulletin, 2021, 37(4): 116-126. (in Chinese with English abstract) DOI
[21]	乔江涛, 郭荣波, 袁宪正, 等. 玉米秸秆厌氧降解复合菌系的微生物群落结构[J]. 环境科学, 2013, 34(4): 1531-1539.
	QIAO J T, GUO R B, YUAN X Z, et al. Phylogenetic analysis of methanogenic corn stalk degrading microbial communities[J]. Environmental Science, 2013, 34(4): 1531-1539. (in Chinese with English abstract)
[22]	康志超. 耐低温木质纤维素降解菌群的构建及其应用研究[D]. 哈尔滨: 中国科学院东北地理与农业生态研究所, 2019.
	KANG Z C. Construction and functional evaluation of novel lignocellulose degradation microbial consortia at low temperature[D]. Harbin: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 2019. (in Chinese with English abstract)
[23]	NAKHATE S P, GUPTA R K, PODDAR B J, et al. Influence of lignin level of raw material on anaerobic digestion process in reorganization and performance of microbial community[J]. International Journal of Environmental Science and Technology, 2022, 19(3): 1819-1836. DOI URL
[24]	陈建军, 刘梁涛, 曹香林. 高效木质素降解菌的筛选及产漆酶条件的研究[J]. 甘肃农业大学学报, 2018, 53(4): 130-136.
	CHEN J J, LIU L T, CAO X L. Study on screening of efficient lignin degrading bacteria and the conditions for laccase production[J]. Journal of Gansu Agricultural University, 2018, 53(4): 130-136. (in Chinese with English abstract)
[25]	JIANG C, CHENG Y, ZANG H L, et al. Biodegradation of lignin and the associated degradation pathway by psychrotrophic Arthrobacter sp. C2 from the cold region of China[J]. Cellulose, 2020, 27(3): 1423-1440. DOI URL
[26]	崔堂武, 袁波, 凌晨, 等. 木质素降解酶的酶活测试方法的评价与分析[J]. 化工进展, 2020, 39(12): 5189-5202.
	CUI T W, YUAN B, LING C, et al. Evaluation and analysis of activity assays of ligninolytic enzymes[J]. Chemical Industry and Engineering Progress, 2020, 39(12): 5189-5202. (in Chinese with English abstract)
[27]	RUIJSSENAARS H J, HARTMANS S. A cloned Bacillus halodurans multicopper oxidase exhibiting alkaline laccase activity[J]. Applied Microbiology and Biotechnology, 2004, 65(2): 177-182.
[28]	MEUX E, PROSPER P, MASAI E, et al. Sphingobium sp. SYK-6 LigG involved in lignin degradation is structurally and biochemically related to the glutathione transferase Omega class[J]. FEBS Letters, 2012, 586(22): 3944-3950. DOI URL
[29]	QI Y, LIU H, WANG J, et al. Effects of different straw biochar combined with microbial inoculants on soil environment in pot experiment[J]. Scientific Reports, 2021, 11(1): 14685. DOI PMID
[30]	萨如拉, 高聚林, 于晓芳, 等. 玉米秸秆低温降解复合菌系的筛选[J]. 中国农业科学, 2013, 46(19): 4082-4090.
	SARULA, GAO J L, YU X F, et al. Screening of low temperature maize stalk decomposition microorganism[J]. Scientia Agricultura Sinica, 2013, 46(19): 4082-4090. (in Chinese with English abstract)
[31]	JIMÉNEZ D J, KORENBLUM E, ELSAS J D. Novel multi species microbial consortia involved in lignocellulose and 5-hydroxymethylfurfural bioconversion[J]. Applied Microbiology and Biotechnology, 2014, 98(6): 2789-2803. DOI URL

Optimization of low temperature resistant corn stalk degrading bacterial community and its effect

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 31

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HUANG Zheng, ZHANG Rongping, MA Peng, ZHANG Qi, ZHOU Ningning, ASHEN Rigui, FENG Tingyu, ZHOU Lin. Effects of rape straw returning in winter paddy field and nitrogen fertilizer management on dry matter accumulation and yield of hybrid rice [J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 983-991.
[2]	ZHAO Yunyan, SUN Jian, LIANG Junchao, WANG Zhiqi, YAN Tingxian, YAN Xiaowen, WEI Wenliang, LE Meiwang. Effects of low temperature on seedling growth at sesame early seedling period and screening of low-temperature tolerant materials [J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 752-768.
[3]	XU Yang, REN Yilin, WANG Haojie, HUANG Qiuhang, XING Boyuan, CAO Hongliang. Comprehensive evaluation of rape straw biochar as slow-release carrier under different preparation conditions [J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 893-902.
[4]	YU Qiaogang, JIANG Mingbei, SUN Wanchun, HUANG Zhengchen, WANG Feng, WANG Qiang, MA Junwei. Effects of straw mulching and green manure planting on nitrogen and phosphorus runoff loss in hilly tea garden [J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 903-912.
[5]	LI Yanan, YE Wenxing, ZHU Xiangde, CHEN Lin, XU Xiaofeng, ZHANG Lili. LC-MS/MS-based study on effect of rice straw instead of partial corn silage on plasma metabolites of dairy cows [J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 266-274.
[6]	LIU Xiaofen, LING Xiaoqi, XIANG Lili, YU Lu, SHEN Hong, LI Fang. Effect of temperature and gibberellin on flowering regulation of Cymbidium goeringii [J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 355-363.
[7]	LYU Jing, WU Zhiyong, GUO Xiaonong, FENG Yulan, LU Jianxiong, CHAI Weiwei. Optimization of fermented quinoa straw with lactic acid bacteria by response surface methodology [J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1866-1876.
[8]	DING Dongxia, LI Nenghui, LI Jing, TANG Chaonan, WANG Cheng, NIU Tianhang, YANG Yan, YANG Haitao, XIE Jianming. Effects of exogenous melatonin on chlorophyll fluorescence and antioxidant system of pepper (Capsicum annuum L.) under low temperature and low light stress [J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1935-1944.
[9]	YANG Xiaofang, LI Yunduan, SUN Yunfan, LI Shaojia, MIAO Lixiang, ZHANG Yuchao, JIANG Guihua. Influence of substrate cultivation and soil cultivation on sucrose and citric acid accumulation of Yuexin strawberry [J]. Acta Agriculturae Zhejiangensis, 2022, 34(7): 1423-1430.
[10]	LI Wenxiang, WANG Fang, WANG Jian. Cloning and target gene screening of miR397 in potato [J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1141-1151.
[11]	ZHU Ming, LIU Chen, LIN Yicheng, GUO Bin, LI Hua, FU Qinglin. Effects of conditioning agents on soil fertility, microbial community diversity and rice yield in red soil [J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1258-1267.
[12]	LI Xiaolan, ZHANG Rui, HAO Lanlan, WANG Hong. Bioinformatics analysis of peach NAC gene family and its expression characteristics in response to low temperature stress [J]. Acta Agriculturae Zhejiangensis, 2022, 34(4): 766-780.
[13]	TANG Weidong, LIU Zhenwen, LIU Dongsheng, HU Xuehua. Effects of low temperature and weak light stress on leaf area and dry matter of cucumber in greenhouse [J]. Acta Agriculturae Zhejiangensis, 2022, 34(3): 517-524.
[14]	WANG Jingge, JI Xiaofeng, WU Jing, YANG Hua, TANG Biao, DING Bao'an. Effects of sulfamonomethoxine on bacterial community structure in feces of laying hens [J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 284-292.
[15]	LIN Zhiwen, ZHANG Peng, WU Tianhao, SHAN Ying, ZOU Ganghua, ZHAO Fengliang, ZHENG Guiping. Effects of straw and straw-derived biochar returning on ammonia volatilization in tropical soil-rice system [J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2689-2699.