Optimization of low temperature resistant corn stalk degrading bacterial community and its effect

doi:10.3969/j.issn.1004-1524.2022.12.15

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (12): 2720-2727.DOI: 10.3969/j.issn.1004-1524.2022.12.15

• Environmental Science • Previous Articles Next Articles

Optimization of low temperature resistant corn stalk degrading bacterial community and its effect

WANG Yiran¹^,²(), KANG Zhichao¹, ZHU Guopeng¹, WANG Yang³, QI Geqi³^,^*(), YU Hongwen¹^,^*()

1. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China
2. School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun 130022, China
3. Hinggan League Institute of Agricultural and Animal Husbandry Sciences, Agriculture and Animal Husbandry Bureau of Hinggan League, Hinggan 137400, Inner Mongolia, China

Received:2022-03-22 Online:2022-12-25 Published:2022-12-26
Contact: QI Geqi,YU Hongwen

Abstract

Abstract:

In order to adapt to the climate characteristics of low average annual temperature and frequent freezing and thawing in northeast China, this study optimized the construction of microbial community which could efficiently degrade straw in low temperature environment. The metagenomic sequencing technology was employed for correlation analysis of six bacterial genera (Sphingobacter, Arthrobacter, Flavobacterium, Geobacter, Delfteia and Sphingosphinomonas) in JZ5, an original frigostable straw degrading bacterial community. Besides, the composition of the original community JZ5 was simplified. The results showed that after 12 d of culture at 15 ℃, pH value of optimized community CJZ1 and CJZ2 increased by 0.76 and 0.66 compared with the initial value, respectively, and the degradation rate of straw increased by 0.47 and 0.25 percentage point compared with the original community, respectively, with no significant difference. In addition, the active peak of laccase and lignin peroxidase significantly decreased, while cellulase activity increased by 43.66% and 37.99%, hemicellulase activity increase by 21.40% and 12.91%, respectively in CJZ1 and CJZ2. The results could provide a new idea for the optimization of bacterial community, and a high quality bacterial community for the efficient degradation of straw at low temperature.

Key words: low temperature, straw, bacterial community, degradation rate, Lignin peroxidase, cellulase, hemicellulase

CLC Number:

S141.4
X712

WANG Yiran, KANG Zhichao, ZHU Guopeng, WANG Yang, QI Geqi, YU Hongwen. Optimization of low temperature resistant corn stalk degrading bacterial community and its effect[J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2720-2727.

Figures/Tables 7

Table 1 Bacteria composition

分组 Group	不同属的菌株数量Number of strains from different genera
分组 Group	鞘氨醇杆菌 Sphingobacter	代尔夫特菌 Delfteia	鞘氨醇单胞菌 Sphingosphinomonas	地杆菌 Geobacter	黄杆菌 Flavobacterium	节杆菌 Arthrobacter
JZ5	10	1	1	4	2	2
CJZ1	10	1	1	4	2	0
CJZ2	10	1	1	4	0	0

Fig.1 pH of different bacterial communities

Fig.2 Laccase activity of different bacterial communities

Fig.3 Lignin peroxidase activity of different bacterial communities

Fig.4 Cellulase activity curve of different bacterial communities

Fig.5 Hemicellulase activity of different bacterial communities

Fig.6 Degradation rate of straw by different bacterial communities

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Optimization of low temperature resistant corn stalk degrading bacterial community and its effect

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