Genome-wide identification and expression analysis of ALMT gene family in radish

doi:10.3969/j.issn.1004-1524.2022.04.11

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (4): 746-755.DOI: 10.3969/j.issn.1004-1524.2022.04.11

• Horticultural Science • Previous Articles Next Articles

Genome-wide identification and expression analysis of ALMT gene family in radish

LIU Tongjin¹(), XU Mingjie¹, WANG Jinglei², LIU Liangfeng³, CUI Qunxiang¹, BAO Chonglai²^,^*(), WANG Changyi¹^,^*()

1. College of Horticulture, Jinling Institute of Technology, Nanjing 210038, China
2. Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. Agricultural and Rural Affairs Bureau of Jiangning District, Nanjing 211100, China

Received:2021-07-30 Online:2022-04-25 Published:2022-04-28
Contact: BAO Chonglai,WANG Changyi

Abstract

Abstract:

In order to explore the response of aluminium activated malate transporter (ALMT) genes to biotic and abiotic stress in radish,ALMT genes of radish were genome-wide identified by bioinformatics method and expression patterns were analyzed based on RNA-seq data. The results showed that there were 17 ALMT genes in radish genome, and distributed on eight chromosomes. Exon number of these genes ranged from 5 to 7, and 5-6 transmembrane domains were predicted in their N-terminal region. Number of RsALMT genes on chromosomes was different. Expression analysis of RsALMT genes in different tissues and developments stages,treatment with Agrobacterium tumefaciens and heavy metal (cadmium, chromium and lead) showed that RsALMT1 and RsALMT13 might be involved in resistance to the infection of A. tumefaciens, RsALMT3 might control stomatal open, while RsALMT7, RsALMT11 and RsALMT14 mightbe involved in stomatal closure.RsALMT15 might regulate the accumulation of chlorophyll in flesh of radish and RsALMT16 might be involved in resistance to leadstress.Therefore, the above results would provide a theoretical basis for further study on the functions and molecular mechanisms of radish ALMT family members in response to biotic and abiotic stress.

Key words: radish, ALMT gene family, phylogenetic evolution, gene expression

CLC Number:

S631.1

LIU Tongjin, XU Mingjie, WANG Jinglei, LIU Liangfeng, CUI Qunxiang, BAO Chonglai, WANG Changyi. Genome-wide identification and expression analysis of ALMT gene family in radish[J]. Acta Agriculturae Zhejiangensis, 2022, 34(4): 746-755.

Figures/Tables 9

References 38

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基因名称 Gene name	登录号 Accession No.	氨基酸数量 Number of amino acids/aa	分子量 Molecular weight/u	等电点 Isoeletric point	不稳定指数 Instability index	亲水性 GRAVY	脂肪指数 Aliphatic index	跨膜结构 Transmembrane domain	拟南芥同源基因 Homologue of AtALMT
RsALMT1	Rsa10013723	525	58 320.75	6.40	36.07	0.010	91.22	5	AT2G17470
RsALMT2	Rsa10016259	542	60 681.27	8.99	42.72	-0.072	96.88	5	AT5G46600
RsALMT3	Rsa10035068	621	70 102.62	6.18	36.65	-0.043	89.98	5	AT3G18440
RsALMT4	Rsa10034640	491	54 643.38	8.77	32.83	-0.027	99.61	5	AT3G11680
RsALMT5	Rsa10021670	479	53 569.17	8.30	36.44	0.014	89.37	5	AT4G00910
RsALMT6	Rsa10022591	481	53 733.47	9.04	32.19	-0.020	90.58	6	AT4G00910
RsALMT7	Rsa10025402	543	60 482.70	8.84	40.46	-0.071	95.91	6	AT4G17970
RsALMT8	Rsa10010851	618	68 136.96	7.59	38.48	-0.174	90.95	5	AT5G46610
RsALMT9	Rsa10020182	404	45 923.80	8.41	30.84	-0.125	94.03	6	AT2G27240
RsALMT10	Rsa10011190	489	54 469.32	9.10	32.90	0.032	104.03	5	AT3G11680
RsALMT11	Rsa10039953	580	65 364.87	7.21	40.72	-0.098	86.60	6	AT1G25480
RsALMT12	Rsa10038217	603	67 587.76	6.32	46.06	-0.036	90.10	6	AT1G18420
RsALMT13	Rsa10037498	505	56 082.17	6.53	40.90	-0.049	97.84	5	AT1G08440
RsALMT14	Rsa10040558	515	58 157.37	6.11	41.97	-0.104	88.62	5	AT1G25480
RsALMT15	Rsa10041290	510	56 863.94	6.69	38.69	-0.123	94.43	5	AT1G08440
RsALMT16	Rsa10041291	471	52 544.32	6.11	42.05	-0.020	98.09	5	AT1G08430
RsALMT17	Rsa10003573	484	54 188.99	8.59	31.96	0.067	93.22	6	AT4G00910

基因名称 Gene name	登录号 Accession No.	氨基酸数量 Number of amino acids/aa	分子量 Molecular weight/u	等电点 Isoeletric point	不稳定指数 Instability index	亲水性 GRAVY	脂肪指数 Aliphatic index	跨膜结构 Transmembrane domain	拟南芥同源基因 Homologue of AtALMT
RsALMT1	Rsa10013723	525	58 320.75	6.40	36.07	0.010	91.22	5	AT2G17470
RsALMT2	Rsa10016259	542	60 681.27	8.99	42.72	-0.072	96.88	5	AT5G46600
RsALMT3	Rsa10035068	621	70 102.62	6.18	36.65	-0.043	89.98	5	AT3G18440
RsALMT4	Rsa10034640	491	54 643.38	8.77	32.83	-0.027	99.61	5	AT3G11680
RsALMT5	Rsa10021670	479	53 569.17	8.30	36.44	0.014	89.37	5	AT4G00910
RsALMT6	Rsa10022591	481	53 733.47	9.04	32.19	-0.020	90.58	6	AT4G00910
RsALMT7	Rsa10025402	543	60 482.70	8.84	40.46	-0.071	95.91	6	AT4G17970
RsALMT8	Rsa10010851	618	68 136.96	7.59	38.48	-0.174	90.95	5	AT5G46610
RsALMT9	Rsa10020182	404	45 923.80	8.41	30.84	-0.125	94.03	6	AT2G27240
RsALMT10	Rsa10011190	489	54 469.32	9.10	32.90	0.032	104.03	5	AT3G11680
RsALMT11	Rsa10039953	580	65 364.87	7.21	40.72	-0.098	86.60	6	AT1G25480
RsALMT12	Rsa10038217	603	67 587.76	6.32	46.06	-0.036	90.10	6	AT1G18420
RsALMT13	Rsa10037498	505	56 082.17	6.53	40.90	-0.049	97.84	5	AT1G08440
RsALMT14	Rsa10040558	515	58 157.37	6.11	41.97	-0.104	88.62	5	AT1G25480
RsALMT15	Rsa10041290	510	56 863.94	6.69	38.69	-0.123	94.43	5	AT1G08440
RsALMT16	Rsa10041291	471	52 544.32	6.11	42.05	-0.020	98.09	5	AT1G08430
RsALMT17	Rsa10003573	484	54 188.99	8.59	31.96	0.067	93.22	6	AT4G00910

Genome-wide identification and expression analysis of ALMT gene family in radish

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