Screening, identification and biocontrol effect of endophytic fungus from Lycium barbarum

doi:10.3969/j.issn.1004-1524.20221161

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (7): 1662-1671.DOI: 10.3969/j.issn.1004-1524.20221161

• Plant Protection • Previous Articles Next Articles

Screening, identification and biocontrol effect of endophytic fungus from Lycium barbarum

HOU Caixia¹^,²(), DING Dedong¹^,², HE Jing¹^,²^,^*(), ZHAO Jitao¹^,², LI Yanxiang¹^,², ZHAO Qian¹^,², ZHANG Chongqing¹^,², LI Nan¹^,²

1. College of Forestry, Gansu Agricultural University, Lanzhou 730070, China
2. Gansu Lycium barbarum Harmless Cultivation Engineering Research Center, Lanzhou 730070, China

Received:2022-08-11 Online:2023-07-25 Published:2023-08-17
Contact: HE Jing

Abstract

Abstract:

In order to screen antagonistic strains with significant inhibitory effects against Fusarium solani, the main pathogenic fungus of Lycium barbarum root rot, endophytic fungi were isolated from healthy L. barbarum plants using tissue separation method. Antagonistic strains were selected through plate confrontation method, and their taxonomic status was determined based on morphological features and molecular biology identification. The effects of endophytic fungi treated with different culture media on the mycelial growth and spore germination of F. solani were compared by mycelial growth rate method and droplet method, and their biocontrol effects were verified by pot experiments. The results showed that out of the 106 endophytic fungi isolated, one antagonistic strain YBG8 was selected, which had a significant inhibitory effect against F. solani, and was identified as Alternaria alternata. The inhibition rate of YBG8 against F. solani mycelial growth was the highest (70.49%) at a volume fraction of 20%, and showed concentration dependence. When YBG8 filtrate volume fraction was 50%, the inhibition rate against F. solani spore germination was the highest (82.4%). Pot experiments showed that pre-inoculation with A. alternata followed by F. solani could effectively reduce the incidence of L. barbarum root rot, achieving a relative preventive effect of up to 50%. These findings provided a theoretical basis for the effective use of endophytic fungi to control L. barbarum root rot.

Key words: Lycium barbarum, root rot, endophytic fungus, antagonistic strain, disease incidence

CLC Number:

S182

HOU Caixia, DING Dedong, HE Jing, ZHAO Jitao, LI Yanxiang, ZHAO Qian, ZHANG Chongqing, LI Nan. Screening, identification and biocontrol effect of endophytic fungus from Lycium barbarum[J]. Acta Agriculturae Zhejiangensis, 2023, 35(7): 1662-1671.

Figures/Tables 8

Fig.1 Inhibition of F. solani by 5 endophytic fungi

Fig.2 Inhibition rate of 5 antagonistic strains to F. solani Data ($\bar{x}$±s) on the bars marked without the same lowercase letter indicated significant differences at P<0.05.

Fig.3 Morphological characteristics of YBG8 strain A, The front of YBG8 colony morphology cultured for 3 days; B, The back of YBG8 colony morphology cultured for 3 days; C, The front of YBG8 colony morphology cultured for 7 days; D, The back of YBG8 colony morphology cultured for 7 days; E and F, Mycelia and spore morphology of YBG8.

Fig.4 Phylogenetic tree of YBG8 and other strains based on rDNA-ITS sequences

Table 1 Inhibition rate of culture solution, filtrate solution and inactivated solution of YBG8 on F. Solani mycelia %

体积分数 Volume fraction/%	对F. solani的抑制率 Inhibition rate on F. Solani mycelia
体积分数 Volume fraction/%	培养原液 Culture solution	培养滤液 Filtrate solution	失活液 Inactivated solution
1.25	31.40±0.44	0.91±0.54	0.13±0.01
2.50	34.54±0.28	3.01±0.54	0.12±0.01
5	37.04±0.58	4.34±0.61	0.11±0.01
10	49.54±0.17	8.22±0.85	0.10±0.01
20	70.49±0.63	18.75±0.73	0.10±0.01

Fig.5 Effect of fermentation filtrate of strain YBG8 on spore germination of F. solani

Fig.6 Control effect of YBG8 strain on L.barbarum root rot A, F. solani; B, Control; C, YBG8; D, After 7 days of inoculation with YBG8 strain, F. solani was inoculated.

Table 2 Control effect of culture solution of YBG8 strain on wolfberry root rot %

处理 Treatment	发病率 Morbidity	病情指数 Disease index	相对防效 Relative control effect
CK	50.0	40.0
YBG8	40.0	36.0	38.8
YBG8+F. solani	30.0	30.0	50.0
F. solani	70.0	60.0	-40
精甲·咯·嘧菌	20	20	66.7
Metalaxyl-M·fludioxonil· azoxystrobin

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Screening, identification and biocontrol effect of endophytic fungus from Lycium barbarum

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