Screening and identification of soybean rhizosphere growth-promoting bacteria and their growth-promoting effects

doi:10.3969/j.issn.1004-1524.20221786

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (12): 2775-2784.DOI: 10.3969/j.issn.1004-1524.20221786

• Crop Science • Previous Articles Next Articles

Screening and identification of soybean rhizosphere growth-promoting bacteria and their growth-promoting effects

LIU Yue(), XU Weihui, WANG Zhigang()

College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, Heilongjiang, China

Received:2022-12-15 Online:2023-12-25 Published:2023-12-27

Abstract

Abstract:

The soybean rhizosphere soil was used as experimental material to screen efficient growth-promoting strains, then enriching the strain resources of soybean special biofertilizer. Plant growth-promoting rhizobacteria (PGPR) were selected using selective medium of phosphate dissolving, potassium dissolving, phosphate solubilization, autotrophic nitrogen fixation and combined nitrogen fixation. The abilities of phosphate solubilization, phosphorus dissolving, potassium solubilization, nitrogen fixation, indole-3-acetic acid (IAA) and gibberellin (GA) of PGPR were measured, then the effect of PGPR on the growth of soybean plants was explored. The results showed that 93 PGPR strains were isolated belonging to the genera Priestia, Pseudomonas, Stenotrophomonas, Klebsiella, Enterobacteria, Bacillus, Arthrobacter, and Acinetobacter. Twenty-nine excellent PGPR strains were screened based on their growth-promoting functions and characteristics, among which strains GD6, JP7, JP34, PKO13, JK32, GD3, and GD32 promoted the accumulation of root surface area of soybean, which increased by 42.93%, 48.41%, 53.04%, 54.69%, 47.86%, 50.44%, 48.67%, respectively. Seven of the above strains, 4 Klebsiella, 2 Bacillus, and a Pristeria megagonia, are candidate strains for soybean microbial fertilizers.

Key words: soybean, rhizosphere growth-promoting bacteria, phosphate-solubilizing bacteria, nitrogen-fixing bacteria

CLC Number:

LIU Yue, XU Weihui, WANG Zhigang. Screening and identification of soybean rhizosphere growth-promoting bacteria and their growth-promoting effects[J]. Acta Agriculturae Zhejiangensis, 2023, 35(12): 2775-2784.

Figures/Tables 5

Fig.1 Phylogenetic tree of 16S rDNA gene sequence of strain JP, JK, PKO, GD and LH represent phosphate dissolving bacteria, potassium dissolving bacteria, phosphate solubilizing bacteria, autotrophic nitrogen fixing bacteria and combined nitrogen fixing bacteria, respectively.

Fig.2 Results of determination of phosphate dissolving (a), phosphate solubilizing (b) and potassium dissolving (c) of rhizosphere growth-promoting bacteria

Fig.3 Nitrogen fixation ability of autotrophic nitrogen fixing bacteria (a) and combined nitrogen fixing bacteria (b) The error line marked without the same lower letters indicated significant differences at P<0.05. The same as below.

Fig.4 The ability of combined nitrogen fixing bacteria (a), phosphate solubilizing bacteria (b), autotrophic nitrogen fixing bacteria (c), potassium solubilizing bacteria (d) and phosphate dissoloving bacteria (e) strains to secrete IAA and GA

Fig.5 Promoting effect of rhizosphere growth promoting bacteria on soybean seedlings

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Screening and identification of soybean rhizosphere growth-promoting bacteria and their growth-promoting effects

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