6种杀线剂对重茬山药土壤微生物数量、酶活性和养分含量的影响

doi:10.3969/j.issn.1004-1524.2021.03.16

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (3): 506-515.DOI: 10.3969/j.issn.1004-1524.2021.03.16

6种杀线剂对重茬山药土壤微生物数量、酶活性和养分含量的影响

范琳娟¹, 刘子荣¹, 徐雪亮¹, 王奋山¹, 彭德良², 姚英娟¹^,^*()

1. 江西省农业科学院农业应用微生物研究所,江西南昌330200
2.中国农业科学院植物保护研究所,北京 100094

收稿日期:2020-08-25 出版日期:2021-03-25 发布日期:2021-03-25
作者简介:*, 姚英娟, E-mail: yaoyingjuan@webmail.hzau.edu.cn
范琳娟(1991—),女,河南三门峡人,硕士,研究实习员,研究方向为山药线虫绿色防控。E-mail: fljx99@163.com
通讯作者: 姚英娟
基金资助:
江西省农业科研协同创新项目(JXXTCX202108);江西省重点研发计划(20203BBF63032);江西省现代农业产业技术体系建设专项(JXARS-19-06)

Effects of six kinds of nematicides on soil microbial population, enzymes activities and nutrients in replanted Chinese yam field

FAN Linjuan¹, LIU Zirong¹, XU Xueliang¹, WANG Fenshan¹, PENG Deliang², YAO Yingjuan¹^,^*()

1. Institute of Applied Agricultural Micro-Organisms, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China

Received:2020-08-25 Online:2021-03-25 Published:2021-03-25
Contact: YAO Yingjuan

摘要/Abstract

摘要：

为明确常用杀线剂对土壤环境的影响,采用土壤常规分析方法,研究2种化学杀线剂(阿维·噻唑膦和氟吡菌酰胺)和4种生物杀线剂(厚孢轮枝菌、淡紫拟青霉、辣根素和茶枯抑线生物菌肥)对土壤微生物数量、酶活性和土壤养分的影响。结果表明,与不施用杀线剂的对照相比,2种化学杀线剂对苗期土壤细菌和真菌数量均表现出显著(P<0.05)的促进作用,而至成熟期后土壤细菌和真菌数量均降至对照水平及以下。4种生物杀线剂中,茶枯抑线生物菌肥对苗期土壤细菌/真菌表现出显著(P<0.05)的抑制作用,对真菌数量表现出显著(P<0.05)的促进作用,在成熟期对土壤细菌数量和细菌/真菌表现出显著(P<0.05)的促进作用,而对真菌数量无显著影响。6种杀线剂施用后对土壤脲酶活性均无显著影响。2种化学杀线剂和生物杀线剂厚孢轮枝菌、淡紫拟青霉和辣根素对土壤酸性磷酸酶活性在苗期和成熟期分别表现出显著(P<0.05)的增强作用和抑制作用,茶枯抑线生物菌肥仅对成熟期的土壤酸性磷酸酶活性表现出显著(P<0.05)的抑制作用。茶枯抑线生物菌肥对苗期和成熟期的土壤蔗糖酶活性分别表现出显著(P<0.05)的抑制作用和促进作用,其他杀线剂则始终表现出显著(P<0.05)的抑制作用。6种杀线剂显著(P<0.05)影响土壤铵态氮、速效钾和速效磷含量。施用杀线剂后,土壤铵态氮和速效钾含量在苗期时与对照相比无显著变化,至成熟期后,除茶枯抑线生物菌肥处理的土壤铵态氮含量外,其他处理的土壤铵态氮和速效钾含量均显著(P<0.05)高于对照。苗期时,施用杀线剂各处理的土壤速效磷含量均显著(P<0.05)低于对照;但成熟期时,各处理的土壤速效磷含量无显著差异。综上,这6种杀线剂对土壤微生物、土壤脲酶和土壤养分的影响随着时间的延长可逐渐恢复,但对土壤酸性磷酸酶和蔗糖酶活性的作用效果持续时间较长,可在一定程度上干扰土壤中的有机磷矿化和碳循环。

关键词: 山药, 杀线剂, 酶活性, 土壤养分

Abstract:

To clarify the effect of nematicides on soil environment, the effects of two kinds of chemical nematicides (avifosthiazate granules and fluopyram) and four kinds of biological nematicides (Verticillium chlamydosporium, Paecilomyces lilacinus, horseradish and tea curd) on soil microbe quantity, enzymes activities and soil nutrients in the replanted Chinese yam field at seedling stage and maturity stage were studied by conventional analysis methods. The results indicated that, compared with the control without nematicides, two kinds of chemical nematicides significantly (P<0.05) increased the number of soil bacteria and fungi at seedling stage, but the number of soil bacteria and fungi all decreased to the same level as control or lower at maturity stage. Among the four kinds of biological nematicides, tea curd significantly (P<0.05) inhibited the ratio of bacteria to fungi (bacteria/fungi), yet significantly (P<0.05) increased the number of fungi at seedling stage, and significantly (P<0.05) increased the number of soil bacteria and bacteria/fungi at maturity stage. All the treatments had no significant effect on soil urease activity. Two kinds of chemical nematicides and V. chlamydosporium, P. lilacinus, horseradish had significant (P<0.05) promoting and inhibiting effects on soil acid phosphatase activity at seedling stage and maturity stage, respectively; while the activity of acid phosphatase was significantly (P<0.05) inhibited by tea curd at maturity stage. The activity of soil sucrase at seedling stage and maturity stage was significantly (P<0.05) inhibited and promoted by the tea curd, respectively, while all the other nematicides showed significant (P<0.05) inhibition effect all the time. The contents of soil ammonium nitrogen, available potassium and available phosphorus were significantly (P<0.05) affected by nematicides. The contents of soil ammonium nitrogen and available potassium had no significant change compared with that of control at seedling stage after application of nematicides. At maturity stage, the contents of ammonium nitrogen and available potassium in other treatments were significantly (P<0.05) higher than those in the control except for the content of ammonium nitrogen with application of tea curd. At seedling stage, the content of soil available phosphorus with application of nematicides was significantly (P<0.05) lower than that of control, but at maturity stage, there was no significant difference in the content of soil available phosphorus. In conclusion, the effects of these nematicides on soil microbial population, soil urease activity and soil nutrients could be gradually recovered with the prolonged time, and showed no potential harm to soil fertility and crop growth, while the effects on soil acid phosphatase activity and sucrase activity might interfere with soil organic phosphorus mineralization and carbon cycle to some extent.

Key words: Chinese yam, nematicides, enzyme activity, soil nutrients

中图分类号:

S154

范琳娟, 刘子荣, 徐雪亮, 王奋山, 彭德良, 姚英娟. 6种杀线剂对重茬山药土壤微生物数量、酶活性和养分含量的影响[J]. 浙江农业学报, 2021, 33(3): 506-515.

FAN Linjuan, LIU Zirong, XU Xueliang, WANG Fenshan, PENG Deliang, YAO Yingjuan. Effects of six kinds of nematicides on soil microbial population, enzymes activities and nutrients in replanted Chinese yam field[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 506-515.

图/表 7

参考文献 34

[1]	涂伟凤, 汤洁, 涂玉琴 , 等. 江西山药生产现状及发展优势[J]. 江西农业学报, 2012,24(11):21-24.
	TU W F, TANG J, TU Y Q , et al. Advantage and status of Chinese yam production in Jiangxi[J]. Acta Agriculturae Jiangxi, 2012,24(11):21-24. (in Chinese with English abstract)
[2]	刘廷辉, 贾海民, 李瑞军 , 等. 6种药剂对山药种薯短体线虫的防治效果[J]. 农药, 2017,56(6):450-452.
	LIU T H, JIA H M, LI R J , et al. Control effect of six kinds of insecticides against Pratylenchus on the yam seed[J]. Agrochemicals, 2017,56(6):450-452.(in Chinese with English abstract)
[3]	贺哲, 黄婷, 李俊科 , 等. 瑞昌山药根腐线虫病病原鉴定[J]. 江西农业大学学报, 2016,38(5):879-883.
	HE Z, HUANG T, LI J K , et al. Identification of nematode causing yam root rot in Ruichang City, Jiangxi Province[J]. Acta Agriculturae Universitatis Jiangxiensis, 2016,38(5):879-883.(in Chinese with English abstract)
[4]	谢平 . 生物肥料“宁盾”对药用植物根结线虫病防治及促生研究[D]. 南京: 南京农业大学, 2015.
	XIE P . Research on promotion and biocontrol of root-knot disease on medicinal plants by bio-fertilizer “NS”[D]. Nanjing: Nanjing Agricultural University, 2015. (in Chinese with English abstract)
[5]	石鸿文, 谢风超 . 山药根结线虫病综合防治技术[J]. 河南农业科学, 2003,32(3):42.
	SHI H W, XIE F C . Integrated control technology of root-knot nematode in Chinese yam[J]. Journal of Henan Agricultural Sciences, 2003,32(3):42.(in Chinese)
[6]	CLAUDIUS-COLE A O, FAWOLE B, ASIEDU R , et al. Management of Meloidogyne incognita in yam-based cropping systems with cover crops[J]. Crop Protection, 2014,63:97-102. DOI URL
[7]	CLAUDIUS-COLE A O, OMOTAYO T O, MONTES A L . Nodal vine cutting technique for assessing nematode resistance in yams[J]. Tropical Plant Pathology, 2020,45(1):56-63. DOI URL
[8]	黄婷, 蒋军喜, 余国庆 , 等. 山药病害最新研究进展[J]. 生物灾害科学, 2014,37(1):74-78.
	HUANG T, JIANG J X, YU G Q , et al. Latest advance in researches on Dioscorea opposite thunb diseases[J]. Biological Disaster Science, 2014,37(1):74-78.(in Chinese with English abstract)
[9]	迟元凯, 叶梦迪, 赵伟 , 等. 氟吡菌酰胺对南方根结线虫的作用效果[J]. 植物保护学报, 2019,46(6):1364-1370.
	CHI Y K, YE M D, ZHAO W , et al. Effect of fluopyram on root-knot nematode Meloidogyne incognita[J]. Journal of Plant Protection, 2019,46(6):1364-1370.(in Chinese with English abstract)
[10]	董文芳, 刘廷辉, 贾海民 , 等. 3种药剂对山药短体线虫病的田间防治效果[J]. 河北农业科学, 2017,21(1):46-48.
	DONG W F, LIU T H, JIA H M , et al. Field efficacy of three nematocides on the shortbody nematode of Dioscorea opposite thunb[J]. Journal of Hebei Agricultural Sciences, 2017,21(1):46-48. (in Chinese with English abstract)
[11]	FASKE T R, HURD K . Sensitivity of Meloidogyne incognita and Rotylenchulus reniformis to fluopyram[J]. Journal of Nematology, 2015,47(4):316-321. URL PMID
[12]	时立波, 王振华, 刘静 , 等. 几种杀线剂对土壤微生物数量及真菌多样性影响[J]. 农药, 2008,47(12):917-919.
	SHI L B, WANG Z H, LIU J , et al. Effects of different nematicides on number of soil microorganism and fungi diversity[J]. Agrochemicals, 2008,47(12):917-919.(in Chinese with English abstract)
[13]	杨叶青, 范琳娟, 刘奇志 , 等. 棉隆和氯化苦熏蒸对重茬草莓土壤线虫群落及养分含量的影响[J]. 园艺学报, 2018,45(4):725-733.
	YANG Y Q, FAN L J, LIU Q Z , et al. Effects of dazomet and chloropicrin on the soil nematode communities and nutrient content of replanted strawberry[J]. Acta Horticulturae Sinica, 2018,45(4):725-733.(in Chinese with English abstract)
[14]	刘淑琮, 冯炘, 于洁 . 植物根际促生菌的研究进展及其环境作用[J]. 湖北农业科学, 2009,48(11):2882-2887.
	LIU S C, FENG X, YU J . Research progress of plant growth-promoting rhizobacteria and its environmental effects[J]. Hubei Agricultural Sciences, 2009,48(11):2882-2887.(in Chinese with English abstract)
[15]	张焕军, 郁红艳, 丁维新 . 长期施用有机无机肥对潮土微生物群落的影响[J]. 生态学报, 2011,31(12):3308-3314.
	ZHANG H J, YU H Y, DING W X . The influence of the long-term application of organic manure and mineral fertilizer on microbial community in calcareous fluvo-aquic soil[J]. Acta Ecologica Sinica, 2011,31(12):3308-3314.(in Chinese with English abstract)
[16]	ELLIS R J, NEISH B, TRETT M W , et al. Comparison of microbial and meiofaunal community analyses for determining impact of heavy metal contamination[J]. Journal of Microbiological Methods, 2001,45(3):171-185. DOI URL PMID
[17]	邹小明, 朱立成, 肖春玲 , 等. 三唑磷的土壤微生物生态效应研究[J]. 农业环境科学学报, 2008,27(1):238-242.
	ZOU X M, ZHU L C, XIAO C L , et al. Ecological effect of triazophos on soil microbe[J]. Journal of Agro-Environment Science, 2008,27(1):238-242.(in Chinese with English abstract)
[18]	武贺, 段玉玺, 陈立杰 . 施用杀线剂对大豆根际真菌的影响[J]. 大豆科学, 2008,27(4):715-719.
	WU H, DUAN Y X, CHEN L J . Effect of nematicide on rhizosphere fungi in the root of soybean[J]. Soybean Science, 2008,27(4):715-719.(in Chinese with English abstract)
[19]	鲍士旦 . 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000.
[20]	刘恩太, 李园园, 胡艳丽 , 等. 棉隆对苹果连作土壤微生物及平邑甜茶幼苗生长的影响[J]. 生态学报, 2014,34(4):847-852. DOI URL
	LIU E T, LI Y Y, HU Y L , et al. Effects of dazomet on edaphon and growth of Malus hupehensis Rehd. under continuous apple cropping[J]. Acta Ecologica Sinica, 2014,34(4):847-852.(in Chinese with English abstract)
[21]	孙秀山, 封海胜, 万书波 , 等. 连作花生田主要微生物类群与土壤酶活性变化及其交互作用[J]. 作物学报, 2001,27(5):617-621.
	SUN X S, FENG H S, WAN S B , et al. Changes of main microbial strains and enzymes activities in peanut continuous cropping soil and their interactions[J]. Acta Agronomica Sinica, 2001,27(5):617-621.(in Chinese with English abstract)
[22]	吴海燕, 范作伟, 刘春光 , 等. 保护性耕作条件下玉米田土壤微生物区系变化与影响因素分析[J]. 玉米科学, 2008,16(4):135-139.
	WU H Y, FAN Z W, LIU C G , et al. Analysis on the regularity and influence factors of change of soil micro flora under maize planting technology of conservation tillage[J]. Journal of Maize Sciences, 2008,16(4):135-139.(in Chinese with English abstract)
[23]	姜莉莉 . 氟醚菌酰胺对番茄四种病害的控制效果及对土壤微生物多样性的影响[D]. 泰安: 山东农业大学, 2015.
	JIANG L L . Control efficacy of LH-2010A on 4 tomato diseases and its effect on soil microbe diversity[D]. Tai'an: Shandong Agricultural University, 2015. (in Chinese with English abstract)
[24]	张盈 . 杀菌剂氟吡菌酰胺对土壤微生物群落多样性的影响[D]. 长沙: 湖南农业大学, 2013.
	ZHANG Y . Effects of fungicide fluopyram on soil microbial community diversity[D]. Changsha: Hunan Agricultural University, 2013. (in Chinese with English abstract)
[25]	LEE S, GAN J Y, KIM J S , et al. Microbial transformation of pyrethroid insecticides in aqueous and sediment phases[J]. Environmental Toxicology and Chemistry, 2004,23(1):1-6. DOI URL PMID
[26]	胡基华, 李晶, 张淑梅 , 等. 解淀粉芽孢杆菌TF28对设施连作黄瓜根际土壤酶活性和微生物的调节[J]. 江苏农业科学, 2020,48(7):152-156.
	HU J H, LI J, ZHANG S M , et al. Regulation of Bacillus amyloliquefaciens TF28 on soil enzyme activities and microorganisms in rhizosphere of cucumber under continuous cropping in greenhouse[J]. Jiangsu Agricultural Sciences, 2020,48(7):152-156.(in Chinese)
[27]	GOMES N C M, FAGBOLA O, COSTA R , et al. Dynamics of fungal communities in bulk and maize rhizosphere soil in the tropics[J]. Applied and Environmental Microbiology, 2003,69(7):3758-3766. DOI URL PMID
[28]	董艳, 董坤, 郑毅 , 等. 种植年限和种植模式对设施土壤微生物区系和酶活性的影响[J]. 农业环境科学学报, 2009,28(3):527-532.
	DONG Y, DONG K, ZHENG Y , et al. Soil microbial community and enzyme activities in greenhouse with different cultivation years and planting system[J]. Journal of Agro-Environment Science, 2009,28(3):527-532.(in Chinese with English abstract)
[29]	纪春涛, 姜兴印, 房锋 , 等. 噻唑膦对冬暖式大棚土壤酶活性的影响[J]. 农药学学报, 2009,11(1):137-140.
	JI C T, JIANG X Y, FANG F , et al. Effects of fosthiazate on activity of soil enzymes in vegetable greenhouse[J]. Chinese Journal of Pesticide Science, 2009,11(1):137-140.(in Chinese with English abstract)
[30]	纪春涛 . 噻唑膦对土壤酶活性及黄瓜幼苗安全性研究[D]. 泰安: 山东农业大学, 2009.
	JI C T . Effects of fosthiazate to soil enzyme and cucumber seedling[D]. Tai'an: Shandong Agricultural University, 2009. (in Chinese with English abstract)
[31]	傅丽君, 杨文金 . 4种农药对枇杷园土壤磷酸酶活性及微生物呼吸的影响[J]. 中国生态农业学报, 2007,15(6):113-116.
	FU L J, YANG W J . Effects of pesticides on soil phosphatase activity and respiration of soil microorganisms in loquat orchard[J]. Chinese Journal of Eco-Agriculture, 2007,15(6):113-116.(in Chinese with English abstract)
[32]	仉欢, 朱玉坤, 乔康 , 等. 磷化铝对土壤微生物数量和酶活性的影响[J]. 农业环境科学学报, 2012,31(1):143-148.
	ZHANG H, ZHU Y K, QIAO K , et al. Effects of aluminum phosphide on soil microbial population and enzyme activities[J]. Journal of Agro-Environment Science, 2012,31(1):143-148.(in Chinese with English abstract)
[33]	贾红梅, 方千, 张秫华 , 等. AM真菌对丹参生长及根际土壤酶活性的影响[J]. 草业学报, 2020,29(6):83-92.
	JIA H M, FANG Q, ZHANG S H , et al. Effects of AM fungi on growth and rhizosphere soil enzyme activities of Salvia miltiorrhiza[J]. Acta Prataculturae Sinica, 2020,29(6):83-92.(in Chinese with English abstract)
[34]	颜冬冬, 王秋霞, 郭美霞 , 等. 4种熏蒸剂对土壤氮素转化的影响[J]. 中国生态农业学报, 2010,18(5):934-938.
	YAN D D, WANG Q X, GUO M X , et al. Effects of four fumigants on soil nitrogen transformation[J]. Chinese Journal of Eco-Agriculture, 2010,18(5):934-938.(in Chinese with English abstract)

处理 Treatment	第一次用药The first time		第二次用药The second time
处理 Treatment	药剂Reagent	用量Dose	药剂Reagent	用量Dose
CK	—	—	—	—
T1	AG	3 g·m^-2	AM	2.25 mL·m^-2
T2	Fl	0.15 mL·m^-2	—	—
T3	Vc	7.5 g·m^-2	—	—
T4	Pl	0.75 g·m^-2	Pl	0.75 g·m^-2
T5	Ho	6 mL·m^-2	—	—
T6	tc	0.15 g·m^-2	—	—

处理 Treatment	第一次用药The first time		第二次用药The second time
处理 Treatment	药剂Reagent	用量Dose	药剂Reagent	用量Dose
CK	—	—	—	—
T1	AG	3 g·m^-2	AM	2.25 mL·m^-2
T2	Fl	0.15 mL·m^-2	—	—
T3	Vc	7.5 g·m^-2	—	—
T4	Pl	0.75 g·m^-2	Pl	0.75 g·m^-2
T5	Ho	6 mL·m^-2	—	—
T6	tc	0.15 g·m^-2	—	—

处理 Treatment	pH	有机质 Organic matter/%	铵态氮 Ammonium nitrogen/ (mg·kg^-1)	速效钾 Available potassium/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)
CK	4.25±0.05 a	1.47±0.32 a	18.00±1.12 ab	40.90±4.45 a	1.64±0.14 a
T1	4.43±0.12 a	1.67±0.32 a	27.73±0.67 a	37.37±3.49 a	0.51±0.01 c
T2	4.45±0.08 a	1.33±0.17 a	11.07±0.54 b	35.43±5.60 a	0.55±0.02 bc
T3	4.43±0.05 a	1.37±0.17 a	24.50±2.60 a	41.23±1.17 a	0.30±0.04 d
T4	4.34±0.07 a	2.07±0.35 a	17.27±2.48 ab	41.53±1.28 a	0.61±0.05 bc
T5	4.62±0.29 a	1.77±0.38 a	22.17±1.24 a	41.37±0.80 a	0.64±0.03 bc
T6	4.72±0.21 a	1.30±0.15 a	24.37±7.16 a	35.27±2.42 a	0.73±0.07 b

处理 Treatment	pH	有机质 Organic matter/%	铵态氮 Ammonium nitrogen/ (mg·kg^-1)	速效钾 Available potassium/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)
CK	4.25±0.05 a	1.47±0.32 a	18.00±1.12 ab	40.90±4.45 a	1.64±0.14 a
T1	4.43±0.12 a	1.67±0.32 a	27.73±0.67 a	37.37±3.49 a	0.51±0.01 c
T2	4.45±0.08 a	1.33±0.17 a	11.07±0.54 b	35.43±5.60 a	0.55±0.02 bc
T3	4.43±0.05 a	1.37±0.17 a	24.50±2.60 a	41.23±1.17 a	0.30±0.04 d
T4	4.34±0.07 a	2.07±0.35 a	17.27±2.48 ab	41.53±1.28 a	0.61±0.05 bc
T5	4.62±0.29 a	1.77±0.38 a	22.17±1.24 a	41.37±0.80 a	0.64±0.03 bc
T6	4.72±0.21 a	1.30±0.15 a	24.37±7.16 a	35.27±2.42 a	0.73±0.07 b

处理 Treatment	pH	有机质 Organic matter/%	铵态氮 Ammonium nitrogen/ (mg·kg^-1)	速效钾 Available potassium/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)
CK	4.77±0.17 ab	0.10±0.01 a	5.67±0.48 e	41.45±2.51 e	ND
T1	5.27±0.02 a	0.13±0.08 a	42.37±1.59 b	74.50±1.33 c	1.00±1.00 a
T2	4.66±0.18 b	0.30±0.06 a	35.57±1.21 c	55.73±4.91 d	0.83±0.33 a
T3	4.71±0.09 b	0.20±0.10 a	15.17±1.13 d	74.03±6.11 c	0.47±0.47 a
T4	4.98±0.21 ab	0.17±0.12 a	14.00±1.79 d	89.55±1.36 b	0.33±0.17 a
T5	4.44±0.25 b	0.13±0.07 a	72.83±4.37 a	108.00±4.62 a	1.73±0.72 a
T6	4.68±0.15 b	0.18±0.02 a	11.33±0.67 de	99.33±0.67 ab	ND

6种杀线剂对重茬山药土壤微生物数量、酶活性和养分含量的影响

Effects of six kinds of nematicides on soil microbial population, enzymes activities and nutrients in replanted Chinese yam field

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 34

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张智, 何豪豪, 郁妙, 许剑锋. 化肥减量配施土壤改良剂对土壤酸度、土壤养分和水稻产量的影响[J]. 浙江农业学报, 2025, 37(6): 1301-1308.
[2]	陈梦微, 梁徐, 张成磊, 梁璟, 许樱子, 项丹丹, 杨照渠, 谢永东. 微生物菌肥对东魁杨梅土壤性状和叶片营养的影响[J]. 浙江农业学报, 2025, 37(5): 1130-1138.
[3]	杜颂, 汤涛, 程曦, 赵学平, 张春荣, 梁晓宇, 王萌, 张震, 李永成, 章程辉. 砜吡草唑及其主要代谢物在土壤中的消解和对土壤酶活性的影响研究[J]. 浙江农业学报, 2025, 37(4): 847-857.
[4]	胡铁军. 化肥减量配施微生物肥对西蓝花产量品质与土壤性质的影响[J]. 浙江农业学报, 2024, 36(7): 1657-1665.
[5]	高兰芸, 刘昊, 李爱, 张婷婷, 杨丽芳, 高英. NaCl对樱桃砧木组培生根、IAA原位分布及相关酶活性的影响[J]. 浙江农业学报, 2024, 36(6): 1300-1308.
[6]	程园, 徐升胜, 段延碧, 徐馨蕾, 郭凤根, 王仕玉, 龙雯虹. 牛尾山药病毒病危害情况调查与茎段脱毒方法[J]. 浙江农业学报, 2024, 36(6): 1329-1338.
[7]	范琳娟, 吴彩云, 徐雪亮, 刘子荣, 姚健, 康宏波, 胡平华, 姚英娟. 两种杀线虫剂处理对山药土壤线虫群落和理化性质的影响[J]. 浙江农业学报, 2024, 36(5): 1102-1112.
[8]	赵小亮, 龙则宇, 鲁雲, 金微微. 山药中部分活性物质的功效研究与应用进展[J]. 浙江农业学报, 2024, 36(4): 920-931.
[9]	宋鹏, 李理想, 江厚龙, 王茹, 李慧, 赵鹏宇, 张均, 秦平伟, 任江波, 陈庆明. 施用侧孢短芽孢杆菌对烤后烟叶钾含量及烟株生理特征的影响[J]. 浙江农业学报, 2024, 36(3): 494-502.
[10]	岳宗伟, 李嘉骁, 孙向阳, 刘国梁, 李素艳, 王晨晨, 查贵超, 魏宁娴. 化肥有机肥配施对土壤性质、樱桃果实品质和产量的影响[J]. 浙江农业学报, 2023, 35(9): 2192-2201.
[11]	吴传美, 何季, 吴文珊, 蔡俊, 向仰州. 间作对刺梨园土壤团聚体化学计量特征和养分贡献率的影响[J]. 浙江农业学报, 2023, 35(5): 1132-1143.
[12]	鲁帅, 罗晓刚, 刘全伟, 张屹, 孟洋昊, 李洁, 张景来. 有机无机复混肥对小麦生长、土壤养分和重金属含量的影响[J]. 浙江农业学报, 2023, 35(4): 922-930.
[13]	张宇昊, 马卫华, 刘晋佳, 马秀梅, 姜玉锁. 亚致死剂量呋虫胺对意大利蜜蜂采集蜂免疫解毒相关基因表达和酶活性的影响[J]. 浙江农业学报, 2023, 35(3): 575-581.
[14]	阮泽斌, 王兰鸽, 蓝王凯宁, 徐彦, 陈俊辉, 柳丹. 氮肥减量配施生物炭对水稻氮素吸收和土壤理化性质的影响[J]. 浙江农业学报, 2023, 35(2): 394-402.
[15]	周力, 桂林生. 饲粮中小麦颗粒用量对藏羊公羔瘤胃内环境的影响[J]. 浙江农业学报, 2023, 35(11): 2543-2554.