›› 2014, Vol. 26 ›› Issue (1): 0-13.

• 作物科学 •    下一篇

一个水稻矮秆窄叶突变体的鉴定和基因定位

刘继云1,2,叶胜海2,李小华2,3,赵小燕1,2,尚海漩1,2,纪现军2,3,邓晓梅2,3,陈萍萍2,金庆生2,张小明2,*   

  1. 1浙江师范大学 化学与生命科学学院,浙江 金华 321004;2浙江省农业科学院 作物与核技术利用研究所,浙江 杭州 310021;3 杭州师范大学 生命与环境科学学院,浙江 杭州 310036
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-01-25 发布日期:2014-07-09

Analysis and mapping of a dwarf and narrow leaf rice mutant

LIU Ji\|yun;YE Sheng\|hai;LI Xiao\|hua;ZHAO Xiao\|yan;SHANG Hai\|xuan;JI Xian\|jun;DENG Xiao\|mei;CHEN Ping\|ping;JIN Qing\|sheng;ZHANG Xiao\|ming;*   

  1. 1College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;2 Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China;3 College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-01-25 Published:2014-07-09

摘要:

常规粳稻秀水09经过甲基磺酸乙酯(EMS)诱变处理,获得了一个矮秆窄叶突变体dnl1(dwarf and narrow leaf 1),经多代自交性状稳定。dnl1突变体与野生型相比,具有植株矮、叶片窄、分蘖强的特点。遗传分析表明dnl1突变性状受1对隐性核基因控制。通过图位克隆的方法,利用SSR分子标记将突变基因dnl1定位在第4号染色体SSR标记RM17478与RM17488之间约260 kb范围内。测序比对分析表明Dnl1可能为Nal1基因.

关键词: 水稻, 矮秆窄叶, 图位克隆, 分子定位

Abstract: A dwarf and narrow leaf (dnl1) mutant was isolated by ethylmethylsulfonate (EMS) treating for a japonica rice variety Xiushui 09 dry seeds,it could be inherited stably after several selfing. Compared with its wild type Xiushui 09, the mutant shows dwarf, narrow leaf and more tillers. Genetic analysis showed that the traits of the dnl1 mutants were controlled by one pair of recessive nuclear gene. With map\|based cloning approach, Dnl1 gene was mapped between SSR marker RM17478 and RM17488 on the chromosome 4, a 260 kb region. Sequencing and blast analysis indicated that Dnl1 is an allele to the gene Nal1.

Key words: rice, dwarf and narrow leaf, map\|based cloning, molecular mapping