关键词: 水稻, 低谷蛋白, Lgc1基因, 分子标记

Abstract: The rice low glutelin content gene Lgc1， carried by the donor parent 04DP， was introduced into the local japonica materials by hybridization， and 52 rice lines of F6 generation were obtained. Two pairs of molecular markers were designed according to the base deletion mutation in Lgc1 gene， then 320 plants from these 52 rice lines were detected by these molecular markers， the glutelin content was determined using Coomassie brilliant blue\|G250 method. Results showed that there were 37 homozygous with Lgc1 gene and glutelin contents of 245%-331%; 9 homozygous with normal glutelin gene and glutelin contents of 355%-476%. The molecular marker detection results were in agreement with the glutelin content. Thus， rice lines with low glutelin content were obtained and the Lgc1 gene marker could be used for marker assisted selection breeding.

Key words: rice, low glutelin, Lgc1 gene, molecular marker