浙江农业学报

• 作物科学 •    下一篇

水稻DNA多态标记的开发与验证

  

  1. (1. 浙江师范大学 化学与生命科学学院, 浙江 金华 321004;2. 中国水稻研究所 水稻生物学国家重点实验室, 浙江 杭州310006)
  • 出版日期:2016-03-25 发布日期:2016-04-07

The development and verification of nucleotide polymorphism markers in rice

  1. (1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China; 2. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China)
  • Online:2016-03-25 Published:2016-04-07

摘要: 利用203对分子标记对3个粳稻品种(日本晴、中花11、武运粳7号)和4个籼稻品种(9311、明恢63、台中1号及南京6号)进行多态性分析,筛选到92对在籼粳间存在差异的标记、34对粳稻品种间存在差异的标记及80对在籼稻品种间存在差异的标记。对部分在多个品种中存在多态性的标记进行测序,结果表明:大部分微卫星标记在不同品种间产物的大小差异是由重复序列重复次数的多少引起,而插入/缺失标记在品种间的序列差异则各有不同,如部分非重复序列的一次或多次重复、长片段的插入或缺失等。这些多态性好、扩增稳定的分子标记的开发和多态性验证为分子标记在种质鉴定、进化分析、遗传多样性检测、分子标记辅助选育等方面的广泛使用奠定了基础。

关键词: 水稻, 分子标记, 多态性, 品种鉴定

Abstract: In this work, polymorphic analysis among 3 japonica cultivars (NIP, ZH11 and WY7) and 4 indica cultivars (9311, MH63, TN1 and NJ6) were conducted with 203 molecular markers, 92 polymorphism markers between japonica cultivars and indica cultivars, 34 polymorphism markers in japonica cultivars and 80 polymorphism markers in indica cultivars had been screened. Sequencing analysis results showed that the polymorphism of microsatellite markers among different varieties was mainly caused by the different repetitions of repetitive sequence. However, the polymorphism of insertion/deletion marks was determined by the repetition times of nonsimple repetitive sequence and insertion or deletion of long fragment. Besides the development of new markers, several RM markers also had been verified whether possessed polymorphism in different varieties. Taken together, these new polymorphism markers were bound to be of great service to use in germplasm identification, evolution, genetic diversity detection and molecular markerassisted selection.

Key words: rice, molecular markers, polymorphism, cultivar identification