›› 2009, Vol. 21 ›› Issue (3): 0-245.

• 论文 •    

杂交早稻保持系沪旱1 B柱头外露率的QTL定位

岳高红 1,梅捍卫 2,潘彬荣 1,楼 珏 2,李明寿 2,罗利军 2,*,   

  1. 1 温州市农业科学院作物研究所,浙江温州325006;2 上海市农业生物基因中心,上海201106
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-25 发布日期:2009-05-25

Mapping of QTLs affecting stigma exsertion rate of Huhan 1B as a CMS maintainer of upland hybrid rice

YUE Gao—hong;MEI Han—Wei;PAN Bin—rong;LOU Jue;LI Ming-shou;LUO Li—jun;*   

  1. 1 Crop Research Institute,Wenzhou Academy of Agricultural Sciences,Wenzhou 325006,China;2 Shanghai Agrobiological Gene Center,Shanghai 201106,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

摘要: 本研究利用沪旱1B/Ⅱ-32B杂交的 群体,对水稻的柱头外露率性状进行了QTL分析,研究其遗传机制,以改良沪旱1B的柱头外露率。试验共检测到1O个与柱头外露率有关的QTLs,分布在4条染色体上,加性效应起主要作用。检测到3个影响单边柱头外露率(PSES)的 ,分别位于3号、7号和9号染色体上,联合贡献率为21.44%;检测到2个影响双边柱头外露率(PDES)的QTLs,位于3号和9号染色体上,联合贡献率为15.98% ;检测到5个影响总柱头外露率(PES)的QTLs,位于3,4,7,9号染色体上,其中3号染色体上有2个位点,总柱头外露率QTL 的单独贡献率在6.86% 一9.73%之间,联合贡献率为39.42%。QTL上位性分析,检测到7对显著互作位点,解释了表型变异的67.39%,检测到的上位性效应主要以加性/加性互作效应为主,部分主效参与了互作。这些QTLs分子标记为改良沪旱1B的柱头外露率性状进行分子标记辅助选择提供参考。

关键词: 水稻, 抗旱杂交稻, 柱头外露率, QTL分析

Abstract: To clarify the genetic mechanism of percentage of stigma exsertion in rice(Oryza sativa L)and improve stigma exsertion rate of a drought tolerant CMS maintainer Huhan 1B.QTL analysis was conducted in a F2 population of 190 plants derived from a cross between Huhan 1B and Ⅱ一32B.Ten QTLs of rice stigma exsertion rate were identified on four chromosomes.Three QTLs of percentage of single exserted stigma(PSES)were mapped on chromosomes 3,7 and 9 with accumulated contribution of 21.4%;Two QTLs of percentage of dual exserted stigma(PDES)were detected on chromosomes 3 and 9 with accumulated contribution of 15.98% ;Five of percentage of total exserted stigma(PES)were mapped on chromosome 3,4,7,9,including 2QTLs on chromosome 3,whose contribution rates varied from 6.86% to 9.73% and
the total contribution rates of the five QTLs was 39.42%.Totally,seven QTLs pairs had significant epistatic effect on the percentage of stigma exsertion rate and accounted for 67.39% of the phenotypie variation.Most of them were additive×additive epistatic effects,and some of major QTLs were involved in the interactions.The identification of the QTLs markers may be important for improvement of Huhan 1B in stigma exsertion rate.

Key words: rice, drought tolerant hybrid rice, percentage of exserted stigma, QTL analysis