Isolation and identification of a myxobacterial strain against Phytophthora infestans and optimization of its fermentation conditions

doi:10.3969/j.issn.1004-1524.20241106

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (12): 2554-2562.DOI: 10.3969/j.issn.1004-1524.20241106

• Plant Protection • Previous Articles Next Articles

Isolation and identification of a myxobacterial strain against Phytophthora infestans and optimization of its fermentation conditions

ZHANG Yu(), DING Yixiu, LI Junda, SHANG Shaojie, ZHAO Xiaojing, HOU Kexin, GAO Yandong, LIU Huirong^*()

College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2024-12-20 Online:2025-12-25 Published:2026-01-09

Abstract

Abstract:

Myxobacteria are an important group of functional microorganisms known for their diverse and abundant metabolites. Phytophthora infestans is the pathogen of potato late blight, which causes great losses to the potato industry in China every year. Therefore, screening for active compounds derived from myxobacterial metabolites that exhibit antagonistic activity against P. infestans holds significant research value. In this study, a myxobacterial strain E12 with remarkable antagonistic activity against P. infestans was isolated from the Ordos region. This strain produced yellow, coral-like fruiting bodies and was identified as Corallococcus coralloides. When cultured in MD1 medium at 32 ℃ without NaCl for 11 days, the addition of resin during fermentation yielded in the largest inhibition zone diameter of 38 mm. The study demonstrated that macroporous resin could effectively adsorb active metabolites from the fermentation solution and enhance their yield. These results provide fundamental data for developing myxobacteria-based biopesticides to control potato late blight.

Key words: myxobacteria, Phytophthora infestans, Corallococcus coralloides, metabolite, fermentation

CLC Number:

S436.32

ZHANG Yu, DING Yixiu, LI Junda, SHANG Shaojie, ZHAO Xiaojing, HOU Kexin, GAO Yandong, LIU Huirong. Isolation and identification of a myxobacterial strain against Phytophthora infestans and optimization of its fermentation conditions[J]. Acta Agriculturae Zhejiangensis, 2025, 37(12): 2554-2562.

Figures/Tables 9

Table 1 Factors for the orthogonal experimental design of strain E12 fermentation conditions

水平 Level	因素Factor
水平 Level	(A)温度 Temperature/℃	(B)时间 Time/d	(C)NaCl质量分数 NaCl mass fraction/%
1	30	9	0
2	32	11	0.5
3	34	13	1.0

Fig.1 Antagonistic plate of the strain E12 against P. infestans and the fruiting bodies of strain E12 A, Plate assay showing the antagonistic activity of strain E12 against Phytophthora infestans; a, Mycelial plug of E12; b, Blank control (VY/2 medium); B, Morphology of the fruiting bodies of strain E12.

Fig.2 Phylogenetic tree of strain E12

Fig.3 Inhibition zone diameter of the fermentation supernatant of strain E12 on different culture media Bars without the same lowercase letter indicate significant (p<0.05) differences. The same as below.

Fig.4 Inhibition zone diameter of fermentation supernatants of strain E12 at different temperatures, NaCl mass fraction and fermentation time

Table 2 Results of orthogonal experiment optimization

实验编号 No.	因素 Factor				抑菌圈直径 Inhibition zone diameter/mm
实验编号 No.	A	B	C	空白Blank	抑菌圈直径 Inhibition zone diameter/mm
1	3	2	3	1	0
2	3	3	1	2	20.00±3.06
3	2	1	3	2	0
4	2	3	2	1	21.30±0.67
5	2	2	1	3	26.00±1.15
6	1	3	3	3	0
7	1	1	1	1	12.00±2.00
8	3	1	2	3	16.00±2.31
9	1	2	2	2	18.00±3.06

Table 3 Results of range analysis

因素	k₁	k₂	k₃	R
A	10.00	16.00	12.00	6.00
B	9.30	14.60	14.00	5.30
C	19.30	18.70	0	19.30
空白Blank	11.30	12.70	14.00	2.70

Fig.5 Inhibition zone diameter of fermentation supernatants against Phytophthora infestans under different fermentation conditions 1, Fermentation condition optimized through the single-factor experiment; 2, Fermentation conditions optimized through the orthogonal experiment.

Fig.6 Inhibition zone diameter of the concentrated fermentation supernatant of strain E12 against Phytophthora infestans A, B, and C represent the concentrated fermentation supernatant without resin addition, the concentrated supernatant after resin filtration, and the concentrated resin eluate, respectively.

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Isolation and identification of a myxobacterial strain against Phytophthora infestans and optimization of its fermentation conditions

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