Abstract: Genetic diversity of 52 soybean germplasm resources had been evaluated by 47 SSR loci in this study. The results indicated that 411 alleles were detected，and the number of alleles was from 3 to 21 and the mean alleles per locus was 8.75. The distribution of Simpson index was 0.5506-0.9357 and the mean Simpson index was 0.8061，meanwhile，the distribution of Shannon-weaver index was 0.9083-2.8440 and the mean Shannon-weaver index was 1.8386. The range of Nei-Li similarity coefficient was from 0.5547 to 0.8589 with average value of 0.6775. It indicated that these materials had abundant genetic diversity. The results of clustering showed that 52 materials were divided into three groups by setting similarity coefficient at 0.666. GroupⅠincluded 20 materials almost from Hangjiahu，Taizhou，Lishui area；GroupⅡ included 24 materials almost from Shaoxing，Jinhua，Quzhou，Lishui area；Group Ⅲ included 8 materials almost from Jinhua，Quzhou area. It indicated that the clustering of SSR had no dose relationship with origins or eco-type of the materials，but were related to plant height or growth habit

Key words: soybean, genetic diversity, SSR, clustering