水稻离体叶片抗纹枯病接种方法的研究

doi:10.3969/j.issn.1004-1524.2016.10.15

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (10): 1730-1737.DOI: 10.3969/j.issn.1004-1524.2016.10.15

水稻离体叶片抗纹枯病接种方法的研究

马晨燕^{1, 2}, 袁正杰², 杨海河^{1, 2}, 曲海艳^{1, 2}, 何海燕², 张玉², 瞿绍洪^{2, *}

1.浙江师范大学化学与生命科学学院, 浙江金华 321004;
2.浙江省农业科学院病毒学与生物技术研究所,浙江杭州 310021

收稿日期:2016-03-15 出版日期:2016-10-15 发布日期:2016-11-20
通讯作者: 瞿绍洪,E-mail:squ@mail.zaas.ac.cn
作者简介:马晨燕(1988—),女,山东菏泽人,硕士研究生,主要从事水稻生物技术研究。E-mail:mcy1025181205@163.com
基金资助:
转基因生物新品种培育重大专项(2012ZX08009001); 浙江省农业科学院科技创新能力提升工程项目(2015CX07); 浙江省农业科学院省部共建国家重点实验室培育基地开放基金(2010DS700124-KF1210,2010DS700124-KF1406)

Studies on the detached-leaf inoculation method for determination of rice resistance to sheath blight (Rhizoctonia solani)

MA Chen-yan^{1, 2}, YUAN Zheng-jie², YANG Hai-he^{1, 2}, QU Hai-yan^{1, 2}, HE Hai-yan², ZHANG Yu², QU Shao-hong^{2, *}

1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China;
2. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2016-03-15 Online:2016-10-15 Published:2016-11-20

摘要/Abstract

摘要： 水稻纹枯病是影响水稻生产的重要病害之一。水稻纹枯病抗性属于数量遗传性状,目前尚未发现高抗或免疫的水稻种质材料。为了提高水稻抗纹枯病种质的筛选和研究效率,对水稻离体叶片抗纹枯病接种的实验条件进行了更精确的控制,并采用了量化的病情调查方法。取纹枯菌接种离体水稻叶片组织进行qRT-PCR分析,水稻病程相关基因呈诱导表达,验证了纹枯病菌对水稻的侵染。采用离体叶片接种、大田成株期接种、苗期“微室”接种法,对抗纹枯病水稻品种和敏感品种进行测试。抗性品种和敏感品种纹枯病病级的统计检验呈显著差异,3种方法的结果表现一致。改进的离体叶片接种方法具有易于操作和重复性好的优点,适用于大规模筛选抗纹枯病水稻种质材料。

关键词: 水稻, 纹枯病, Rhizoctonia solani, 离体叶片法, 病程相关基因

Abstract: Rice sheath blight (Rhizoctonia solani) is one of the important diseases causing large loss to rice production. Rice resistance to R. solani belongs to quantitative genetic trait. Rice germplasm with high resistance or immunity to sheath blight has not yet been identified. In order to improve the efficiency of screening and characterizing sheath blight-resistant rice germplasm, we made precise control of the experimental conditions for the detached-leaf inoculation method, and developed a quantitative protocol for the evaluation of disease severity. The detached leaf tissues inoculated with R. solani were subjected to quantitative reverse-transcription qRT-PCR analysis. The induced expression of rice pathogenesis-related genes verified the infection of the pathogen into rice. A sheath blight-resistant rice cultivar and a sensitive cultivar were tested using the detached-leaf method, the field inoculation method and the micro-chamber inoculation method, respectively. The three methods showed similar results, and disease scores of the resistant and sensitive cultivars were significantly different based on statistical tests. Taken together, the improved detached-leaf inoculation method had the advantages of easy operation and good repeatability, and could be used for large-scale screening of sheath blight-resistant rice germplasm.

Key words: rice (Oryza sativa), sheath blight, Rhizoctonia solani, detached-leaf inoculation, pathogenesis-related gene

中图分类号:

S511

马晨燕, 袁正杰, 杨海河, 曲海艳, 何海燕, 张玉, 瞿绍洪. 水稻离体叶片抗纹枯病接种方法的研究[J]. 浙江农业学报, 2016, 28(10): 1730-1737.

MA Chen-yan, YUAN Zheng-jie, YANG Hai-he, QU Hai-yan, HE Hai-yan, ZHANG Yu, QU Shao-hong. Studies on the detached-leaf inoculation method for determination of rice resistance to sheath blight (Rhizoctonia solani)[J]. , 2016, 28(10): 1730-1737.

参考文献

[1] 孟庆忠, 刘志恒, 王鹤影, 等. 水稻纹枯病研究进展[J]. 沈阳农业大学学报, 2001, 32(5): 376-381.
MENG Q Z, LIU Z H, WANG H Y, et al. Research progress in rice sheath blight [J]. Journal of Shenyang Agricultural University , 2001, 32(5): 376-381. (in Chinese with English abstract)
[2] 左示敏, 张亚芳, 陈宗祥, 等. 水稻抗纹枯病遗传育种研究进展[J]. 中国科学:生命科学, 2010, 40(11): 1014-1023.
ZUO S M, ZHANG Y F, CHEN Z X, et al. Current progress on genetics and breeding in resistance to rice sheath blight[J]. Scientia Sinica Vitae , 2010, 40(11): 1014-1023. (in Chinese)
[3] OKUBARA P A, DICKMAN M B, BLECHIl A E. Molecular and genetic aspects of controlling the soilborne necrotrophic pathogens Rhizoctonia and Pythium [J]. Plant Science , 2014, 228: 61-70.
[4] TAHERI P, TARIGHI S. Cytomolecular aspects of rice sheath blight caused by Rhizoctonia solani [J]. European Journal of Plant Pathology , 2011, 129(4): 511-528.
[5] PINSON S R M, CAPDEVIELLE F M, OARD J H. Confirming QTLs and finding additional loci conditioning sheath blight resistance in rice using recombinant inbred lines [J]. Crop Science , 2005, 45(2): 503-510.
[6] PRASAD B, EIZENGA G C. Rice sheath blight disease resistance identified in Oryza spp . Accessions [J]. Plant Disease , 2008, 92(11): 1503-1509.
[7] 陈夕军, 王玲, 左示敏, 等. 水稻纹枯病寄主—病原物互作鉴别品种与菌株的筛选[J]. 植物病理学报, 2009, 39(5): 514-520.
CHEN X J, WANG L, ZUO S M, et al. Screening of cultivars and isolates for identifying interaction between host and pathogen of rice sheath blight [J]. Acta Phytopathologica Sinica , 2009, 39(5): 514-520. (in Chinese with English abstract)
[8] 徐国娟, 袁正杰, 左示敏, 等. 水稻苗期纹枯病抗性鉴定微室接种技术的改良[J]. 中国水稻科学, 2015, 29(1): 97-105.
XU G J, YUAN Z J, ZUO S M, et al. Improvement of the micro-chamber inoculation method for determination of rice seedling resistance to sheath blight ( Rhizoctonia solani ) [J]. Chinese Journal of Rice Science , 2015, 29(1): 97-105. (in Chinese with English abstract)
[9] 崔华威, 杨艳丽, 黎敬涛, 等. 一种基于Photoshop的叶片相对病斑面积快速测定方法[J]. 安徽农业科学, 2009, 37(22): 10760-10762.
CUI H W, YANG Y L, LI J T, et al. A faster method for measuring relative lesion area on leaves based on software Photoshop [J]. Journal of Anhui Agricultural Sciences , 2009, 37(22): 10760-10762. (in Chinese with English abstract)
[10] SINGH G, KUMAR S, SINGH P. A quick method to isolate RNA from wheat and other carbohydrate-rich seeds [J]. Plant Molecular Biology Reporter , 2003, 21(1): 93a-93f.
[11] WANG H, MENG J, PENG X, et al. Rice WRKY 4 acts as a transcriptional activator mediating defense responses toward Rhizoctonia solani , the causing agent of rice sheath blight [J]. Plant Molecular Biology , 2015, 89(1/2): 157-171.
[12] MUKHOPADHYAY P, TYAGI A K. OsTCP 19 influences developmental and abiotic stress signaling by modulating ABI4-mediated pathways[J]. Scientific Reports , 2015, 5: 9998. doi:10.1038/srep09998.
[13] 王子斌, 左示敏, 李刚, 等. 水稻成株期对纹枯病的抗性表现研究[J]. 吉林农业大学学报, 2011, 33(2): 144-150.
WANG Z B, ZUO S M, LI G, et al. Study of resistance of rice to sheath blight at adult plant stage [J]. Journal of Jilin Agricultural University , 2011, 33(2): 144-150. (in Chinese with English abstract)
[14] 潘学彪, 陈宗祥, 徐敬友, 等. 不同接种调查方法对抗水稻纹枯病遗传研究的影响[J]. 江苏农学院学报, 1997, 18(3): 27-32.
PAN X B, CHEN Z X, XU J Y, et al. The effects of different methods of inoculation and investigation on genetic research of resistance to rice sheath blight [J]. Journal of Jiangsu Agricultural College , 1997, 18 (3): 27-32. (in Chinese with English abstract)
[15] RUSH M C, HOFF B J, MELLRATH W O. A uniform disease rating system for rice disease in the United States [C]// Proceedings of the 16th Rice Technical Working Group, Lake Charles, Louisiana, USA, 1976: 64.
[16] CROUZET J, TROMBIK T, FRAYSSE A S, et al. Organization and function of the plant pleiotropic drug resistance ABC transporter family [J]. Febs Letters , 2006, 580(4): 1123-1130.

水稻离体叶片抗纹枯病接种方法的研究

Studies on the detached-leaf inoculation method for determination of rice resistance to sheath blight (Rhizoctonia solani)

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