茄子脂氧合酶家族基因全基因组鉴定与表达分析

doi:10.3969/j.issn.1004-1524.2021.06.07

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (6): 1025-1034.DOI: 10.3969/j.issn.1004-1524.2021.06.07

茄子脂氧合酶家族基因全基因组鉴定与表达分析

赵国富¹(), 严亚琴², 汪精磊², 魏庆镇², 包崇来²^,^*()

1.台州科技职业学院农业与生物工程学院,浙江温岭318020
2.浙江省农业科学院蔬菜研究所,浙江杭州 310021

收稿日期:2021-01-20 出版日期:2021-06-25 发布日期:2021-06-25
通讯作者: 包崇来
作者简介:*包崇来, baocl@mail.zaas.ac.cn
赵国富(1968—),男,浙江温岭人,硕士,副教授,研究方向为蔬菜遗传育种与教育学。E-mail: zhaogf2009@126.com
基金资助:
浙江省育种专项——茄子新品种选育和育种技术研究(2016C02051-2-1)

Genome-wide identification and expression analysis of LOX gene family in eggplant (Solanum melongena)

ZHAO Guofu¹(), YAN Yaqin², WANG Jinglei², WEI Qingzhen², BAO Chonglai²^,^*()

1. Department of Agricultural and Biological Engineering, Taizhou Vocational College of Science & Technology, Wenling 318020, China
2. Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-01-20 Online:2021-06-25 Published:2021-06-25
Contact: BAO Chonglai

摘要/Abstract

摘要：

脂氧合酶(lipoxygenase, LOX)是一类重要的含非血红素铁的蛋白,在植物生长发育、响应生物和非生物胁迫时起重要作用。为进一步明确茄子中LOX基因及其进化关系,对茄子全基因组进行分析筛选,共鉴定出12个SmeLOX基因。它们分布在茄子1、3、8和9号染色体上。系统发育树分析表明,茄子LOX家族基因可分为9-LOX、type I 13-LOX和type II 13-LOX 3类。此外,利用实时荧光定量PCR对鉴定出的12个茄子SmeLOX基因进行组织表达分析,结果表明,SmeLOX1、SmeLOX4、SmeLOX6和SmeLOX8基因在茄子5种组织(根、茎秆、叶片、花、果实)中均有表达。研究结果为进一步研究SmeLOX基因在茄子生长发育过程中的功能提供了科学依据。

关键词: 茄子, SmeLOX基因, 系统发育树, 基因表达

Abstract:

Lipoxygenase (LOX) is a class of non-heme iron-containing dioxygenase which plays important role in plant growth and biotic/abiotic stress tolerance.To explore LOX family genes and their evolutionary relationship in eggplants, twelve candidates, which were distributed on chromosomes 1, 3, 8, and 9, were identified as SmeLOX gene by genome-wide analysis. Phylogenetic analysis showed that SmeLOX genes were classified into three categories (9-LOX, type I 13-LOX, and type II 13-LOX). In addition, real-time quantitative PCR analysis demonstrated that only the expression of SmeLOX1, SmeLOX4, SmeLOX6, and SmeLOX8 were detected in all tissues of eggplant, including roots, stems, leaves, flowers and fruits. Overall, this study provided scientific basis for further function analysis of the SmeLOX genes during growth and development of eggplant.

Key words: eggplant (Solanum melongena), SmeLOX gene, phylogenetic analysis, gene expression

中图分类号:

S641.1

赵国富, 严亚琴, 汪精磊, 魏庆镇, 包崇来. 茄子脂氧合酶家族基因全基因组鉴定与表达分析[J]. 浙江农业学报, 2021, 33(6): 1025-1034.

ZHAO Guofu, YAN Yaqin, WANG Jinglei, WEI Qingzhen, BAO Chonglai. Genome-wide identification and expression analysis of LOX gene family in eggplant (Solanum melongena)[J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1025-1034.

图/表 7

参考文献 32

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基因Gene	正向引物Forward primer	反向引物Reverse primer
SmeLOX1	TGGATCATCATGACACAGTC	GGATGAGGAAGGCTTAGTTC
SmeLOX2	ATTGAAACCGTTAGCAATTGAA	CTCAAGACCATCTTCATGTGG
SmeLOX3	AGTAACTGCAGGTGAATCAG	CAAGTGTGAGTGACTTGAGA
SmeLOX4	CCCCCACTTAAAAGAGATAGA	AGCAATTCAGGAGTTCCAAA
SmeLOX5	AGGCCTTAACATTCTCCGGC	ATCTGGTAAATGGACTCCAACG
SmeLOX6	AATCAAACATGTCTGGTCAC	TCCCTAGATTTCTCCACCAA
SmeLOX7	TAAGCAGTTTCTCCTTGACC	CTCGTCTAGAGAATGTGTCG
SmeLOX8	AATTTATGGGTATTTCCCTGC	CCTGAATGCTTCTCTTGTCC
SmeLOX9	AGATTTACCTCGGACAAAGG	CAGTATTTGGTCACCATTCC
SmeLOX10	TGAATGTGTTTCAAGGAAGTG	GCTTTTGATTTTGCATGAGTTT
SmeLOX11	GGAACCATGACACTACAGA	ACTTCGTGAAATCGATCAAG
SmeLOX12	TAGCCAGACCTTTTAACGAG	AAATTTATGCAATTGCCCCC
18S RNA	CGCGCGCTACACTGATGTATTCAA	TACAAAGGGCAGGGACGTAGTCAA

基因Gene	正向引物Forward primer	反向引物Reverse primer
SmeLOX1	TGGATCATCATGACACAGTC	GGATGAGGAAGGCTTAGTTC
SmeLOX2	ATTGAAACCGTTAGCAATTGAA	CTCAAGACCATCTTCATGTGG
SmeLOX3	AGTAACTGCAGGTGAATCAG	CAAGTGTGAGTGACTTGAGA
SmeLOX4	CCCCCACTTAAAAGAGATAGA	AGCAATTCAGGAGTTCCAAA
SmeLOX5	AGGCCTTAACATTCTCCGGC	ATCTGGTAAATGGACTCCAACG
SmeLOX6	AATCAAACATGTCTGGTCAC	TCCCTAGATTTCTCCACCAA
SmeLOX7	TAAGCAGTTTCTCCTTGACC	CTCGTCTAGAGAATGTGTCG
SmeLOX8	AATTTATGGGTATTTCCCTGC	CCTGAATGCTTCTCTTGTCC
SmeLOX9	AGATTTACCTCGGACAAAGG	CAGTATTTGGTCACCATTCC
SmeLOX10	TGAATGTGTTTCAAGGAAGTG	GCTTTTGATTTTGCATGAGTTT
SmeLOX11	GGAACCATGACACTACAGA	ACTTCGTGAAATCGATCAAG
SmeLOX12	TAGCCAGACCTTTTAACGAG	AAATTTATGCAATTGCCCCC
18S RNA	CGCGCGCTACACTGATGTATTCAA	TACAAAGGGCAGGGACGTAGTCAA

基因名称 Gene name	基因位置 Gene code	氨基酸长度 Amino acids length/aa	蛋白质相对分子质量 Molecular weight/u	芳香度 Aromaticity	不稳定指数 Instability index	等电点 Isoelectric point	亲水性 Gravy
SmeLOX1	Smechr0101045.1	843	95 774.44	0.09	40.53	5.85	-0.399
SmeLOX2	Smechr0101046.1	836	95 455.10	0.11	42.00	5.60	-0.410
SmeLOX3	Smechr0101047.1	863	97 961.05	0.10	40.19	5.45	-0.404
SmeLOX4	Smechr0104163.1	898	101 947.77	0.11	40.69	6.05	-0.353
SmeLOX5	Smechr0104167.1	899	101 887.71	0.10	37.98	6.12	-0.385
SmeLOX6	Smechr0300687.1	909	103 351.01	0.09	45.09	8.35	-0.404
SmeLOX7	Smechr0300720.1	793	90 715.48	0.09	53.28	6.14	-0.388
SmeLOX8	Smechr0303656.1	908	102 036.25	0.09	40.27	6.67	-0.349
SmeLOX9	Smechr0800437.1	859	96 846.64	0.10	39.79	5.41	-0.353
SmeLOX10	Smechr0901372.1	846	97 216.76	0.10	45.77	8.54	-0.496
SmeLOX11	Smechr0902151.1	886	100 649.19	0.10	41.35	5.89	-0.363
SmeLOX12	Smechr0902152.1	851	97 626.82	0.10	45.39	5.89	-0.433

基因名称 Gene name	基因位置 Gene code	氨基酸长度 Amino acids length/aa	蛋白质相对分子质量 Molecular weight/u	芳香度 Aromaticity	不稳定指数 Instability index	等电点 Isoelectric point	亲水性 Gravy
SmeLOX1	Smechr0101045.1	843	95 774.44	0.09	40.53	5.85	-0.399
SmeLOX2	Smechr0101046.1	836	95 455.10	0.11	42.00	5.60	-0.410
SmeLOX3	Smechr0101047.1	863	97 961.05	0.10	40.19	5.45	-0.404
SmeLOX4	Smechr0104163.1	898	101 947.77	0.11	40.69	6.05	-0.353
SmeLOX5	Smechr0104167.1	899	101 887.71	0.10	37.98	6.12	-0.385
SmeLOX6	Smechr0300687.1	909	103 351.01	0.09	45.09	8.35	-0.404
SmeLOX7	Smechr0300720.1	793	90 715.48	0.09	53.28	6.14	-0.388
SmeLOX8	Smechr0303656.1	908	102 036.25	0.09	40.27	6.67	-0.349
SmeLOX9	Smechr0800437.1	859	96 846.64	0.10	39.79	5.41	-0.353
SmeLOX10	Smechr0901372.1	846	97 216.76	0.10	45.77	8.54	-0.496
SmeLOX11	Smechr0902151.1	886	100 649.19	0.10	41.35	5.89	-0.363
SmeLOX12	Smechr0902152.1	851	97 626.82	0.10	45.39	5.89	-0.433

基因Gene	根Root	花Flower	叶片Leaf	茎秆Stem	果实Fruit
SmeLOX1	0.10±0.05 gE	1.30±0.05 eB	0.60±0.02 hC	0.30±0.09 efD	2.10±0.06 cA
SmeLOX2	0.30±0.02 fD	1.60±0.09 dA	0.50±0.04 hiC	0 fE	1.30±0.01dB
SmeLOX3	0 gC	0 hC	0.90±0.05 gB	2.01±0.04 bA	2.10±0.10 cA
SmeLOX4	1.60±0.05 dC	3.63±0.12 aA	1.20±0.03 fD	0.76±0.02 dE	2.63±0.23 bB
SmeLOX5	0 gE	2.85±0.09 bA	2.30±0.14 dC	0.72±0.03 dC	0.30±0.06 fgD
SmeLOX6	3.20±0.07 bA	0.30±0.05 fD	0.40±0.02 iD	1.69±0.16 cB	0.86±0.04 eC
SmeLOX7	3.60±0.19 aA	0.50±0.03 fC	0.20±0.04 iD	0 fE	1.23±0.08 dB
SmeLOX8	0.62±0.04 eD	3.01±0.12 bB	1.90±0.03 eC	3.70±0.27 aB	6.30±0.27 aA
SmeLOX9	0 gD	2.65±0.15 cA	1.89±0.05 eB	0.30±0.07 efC	0.46±0.05 fC
SmeLOX10	2.60±0.11 cA	0 gC	2.60±0.16 cA	0.16±0.05 fC	2.35±0.01 cB
SmeLOX11	1.50±0.02 dB	0.50±0.05 gD	4.60±0.15 aA	1.30±0.03 dC	0 hE
SmeLOX12	0 gC	0 gC	4.20±0.13 bA	0.55±0.02 efB	0.23±0.01 gC

茄子脂氧合酶家族基因全基因组鉴定与表达分析

Genome-wide identification and expression analysis of LOX gene family in eggplant (Solanum melongena)

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