浙江农业学报 ›› 2023, Vol. 35 ›› Issue (6): 1243-1252.DOI: 10.3969/j.issn.1004-1524.2023.06.02
收稿日期:2022-08-01
出版日期:2023-06-25
发布日期:2023-07-04
作者简介:王鑫彤(1999—),男,辽宁喀左人,硕士研究生,研究方向为农业生态学。E-mail:18340583195@163.com
通讯作者:
*王国骄,E-mail:2001500024@syau.edu.cn
基金资助:
WANG Xintong(
), WAN Zuliang, YANG Zhenzhong, WANG Guojiao*(
)
Received:2022-08-01
Online:2023-06-25
Published:2023-07-04
摘要:
为提高土壤肥力、充分利用秸秆资源,探究秸秆秋季湿耙还田对水稻叶片和土壤碳(C)、氮(N)、磷(P)化学计量特征的影响及其与产量的关系,在辽宁省盘锦市采用随机区组设计,设置秸秆不还田(CK)、1年秸秆秋季湿耙还田(S1)和连续2年秸秆秋季湿耙还田(S2)处理,测定不同生育时期水稻叶片和土壤C、N、P含量,并计算其化学计量比。结果表明:S1和S2处理水稻成熟期的土壤有机碳含量分别比CK显著(P<0.05)提高8.71%和28.36%。S1、S2处理各生育时期的土壤全磷含量均显著高于CK,S2处理分蘖期和成熟期的土壤全氮含量显著高于CK。与CK相比,S1处理各生育时期的土壤碳磷比(C∶P)均显著降低,而S2处理仅拔节期的土壤C∶P显著降低。各处理的土壤氮磷比(N∶P)为2.02~2.74,除成熟期外,其余生育时期各处理的土壤N∶P均表现为S1<S2<CK,且各处理间差异显著。水稻叶片的C、N、P含量未受秸秆还田的显著影响,叶片N∶P为8.68~11.50。叶片碳氮比(C∶N)、C∶P与土壤有机碳、全氮呈显著正相关。与CK相比,S2处理显著提高了水稻的理论产量和实测产量。水稻产量与土壤有机碳、全氮、土壤C∶P和土壤C∶N呈极显著(P<0.01)正相关,与水稻叶片的生态化学计量特征无显著相关性。综上,短期连续秸秆秋季湿耙还田有利于提高稻田土壤的养分含量和水稻产量,但相对较低的叶片和土壤N∶P表明试验区域水稻生长受N限制,因此应在秸秆还田的基础上加强氮肥管理以确保水稻的稳产增产。
中图分类号:
王鑫彤, 万祖粱, 杨振中, 王国骄. 秸秆秋季湿耙还田对水稻不同生育时期叶片-土壤生态化学计量特征的影响[J]. 浙江农业学报, 2023, 35(6): 1243-1252.
WANG Xintong, WAN Zuliang, YANG Zhenzhong, WANG Guojiao. Effects of rice straw returning to fields by wet harrow in autumn on leaf-soil ecological stoichiometry of rice at different growth stages[J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1243-1252.
| 处理 Treatment | 有机碳 Organic carbon/ (g·kg-1) | 全氮 Total nitrogen/ (g·kg-1) | 全磷 Total phosphorus/ (g·kg-1) | 全钾 Total potassium/ (g·kg-1) | 硝态氮 Nitrate nitrogen/ (mg·kg-1) | 铵态氮 Ammonium nitrogen/ (mg·kg-1) | 有效磷 Available phosphorus/ (mg·kg-1) | 速效钾 Available potassium/ (mg·kg-1) |
|---|---|---|---|---|---|---|---|---|
| CK | 15.81±0.74 | 1.60±0.04 | 0.65±0.02 | 22.37±0.38 | 14.84±0.61 | 3.14±0.12 | 40.23±1.56 | 283.04±5.16 |
| S1 | 15.92±0.14 | 1.60±0.05 | 0.71±0.02 | 22.88±0.43 | 16.40±0.87 | 3.34±0.15 | 39.86±2.06 | 320.26±5.75 |
| S2 | 17.05±0.14 | 1.69±0.04 | 0.73±0.03 | 22.94±0.34 | 16.20±0.69 | 3.41±0.21 | 40.93±2.15 | 326.82±2.52 |
表1 供试土壤的基本理化性质
Table 1 Basic physiochemical properties of test soils
| 处理 Treatment | 有机碳 Organic carbon/ (g·kg-1) | 全氮 Total nitrogen/ (g·kg-1) | 全磷 Total phosphorus/ (g·kg-1) | 全钾 Total potassium/ (g·kg-1) | 硝态氮 Nitrate nitrogen/ (mg·kg-1) | 铵态氮 Ammonium nitrogen/ (mg·kg-1) | 有效磷 Available phosphorus/ (mg·kg-1) | 速效钾 Available potassium/ (mg·kg-1) |
|---|---|---|---|---|---|---|---|---|
| CK | 15.81±0.74 | 1.60±0.04 | 0.65±0.02 | 22.37±0.38 | 14.84±0.61 | 3.14±0.12 | 40.23±1.56 | 283.04±5.16 |
| S1 | 15.92±0.14 | 1.60±0.05 | 0.71±0.02 | 22.88±0.43 | 16.40±0.87 | 3.34±0.15 | 39.86±2.06 | 320.26±5.75 |
| S2 | 17.05±0.14 | 1.69±0.04 | 0.73±0.03 | 22.94±0.34 | 16.20±0.69 | 3.41±0.21 | 40.93±2.15 | 326.82±2.52 |
图1 不同处理对土壤有机碳、全氮、全磷含量的影响 柱上无相同大写字母的表示同一时期不同处理间存在显著(P<0.05)差异,无相同小写字母的表示同一处理不同时期间存在显著(P<0.05)差异。下同。
Fig.1 Effects of different treatments on contents of organic carbon, total nitrogen and total phosphorus in soil Bars marked without the same uppercase letters indicate significant (P<0.05) differences within treatments at the same growth stage, while bars marked without the same lowercase letters indicate significant (P<0.05) differences within different stages under the same treatment. The same as below.
| 生育时期Growth stage | 处理Treatment | C/(g·kg-1) | N/(g·kg-1) | P/(g·kg-1) |
|---|---|---|---|---|
| 分蘖期Tillering | CK | 507.24±25.99 Aa | 46.73±1.78 Aa | 4.27±0.40 Aa |
| S1 | 503.00±25.77 Aa | 48.71±1.02 Aa | 4.24±0.29 Aa | |
| S2 | 509.68±16.16 Aa | 47.25±1.56 Aa | 4.23±0.16 Aa | |
| 拔节期Jointing | CK | 524.31±24.70 Aa | 37.08±1.53 Ab | 3.81±0.09 Ab |
| S1 | 520.38±26.96 Aa | 39.48±1.85 Ab | 3.79±0.20 Ab | |
| S2 | 534.95±25.65 Aa | 39.67±1.02 Ab | 4.04±0.19 Ab | |
| 抽穗期Heading | CK | 524.89±17.60 Aa | 28.45±1.76 Ac | 2.80±0.12 Ac |
| S1 | 530.01±25.19 Aa | 27.94±1.25 Ac | 2.65±0.08 Ac | |
| S2 | 508.13±20.24 Aa | 28.25±1.67 Ac | 2.86±0.12 Ac | |
| 成熟期Maturity | CK | 529.07±20.92 Aa | 18.79±1.4 Ad | 2.02±0.10 Ad |
| S1 | 526.97±21.53 Aa | 16.80±0.85 Bd | 1.95±0.15 Ad | |
| S2 | 504.75±23.48 Aa | 17.93±0.41 ABd | 1.96±0.22 Ad |
表2 不同处理对水稻叶片碳、氮、磷含量的影响
Table 2 Effect of different treatments on C, N, P contents of leaves
| 生育时期Growth stage | 处理Treatment | C/(g·kg-1) | N/(g·kg-1) | P/(g·kg-1) |
|---|---|---|---|---|
| 分蘖期Tillering | CK | 507.24±25.99 Aa | 46.73±1.78 Aa | 4.27±0.40 Aa |
| S1 | 503.00±25.77 Aa | 48.71±1.02 Aa | 4.24±0.29 Aa | |
| S2 | 509.68±16.16 Aa | 47.25±1.56 Aa | 4.23±0.16 Aa | |
| 拔节期Jointing | CK | 524.31±24.70 Aa | 37.08±1.53 Ab | 3.81±0.09 Ab |
| S1 | 520.38±26.96 Aa | 39.48±1.85 Ab | 3.79±0.20 Ab | |
| S2 | 534.95±25.65 Aa | 39.67±1.02 Ab | 4.04±0.19 Ab | |
| 抽穗期Heading | CK | 524.89±17.60 Aa | 28.45±1.76 Ac | 2.80±0.12 Ac |
| S1 | 530.01±25.19 Aa | 27.94±1.25 Ac | 2.65±0.08 Ac | |
| S2 | 508.13±20.24 Aa | 28.25±1.67 Ac | 2.86±0.12 Ac | |
| 成熟期Maturity | CK | 529.07±20.92 Aa | 18.79±1.4 Ad | 2.02±0.10 Ad |
| S1 | 526.97±21.53 Aa | 16.80±0.85 Bd | 1.95±0.15 Ad | |
| S2 | 504.75±23.48 Aa | 17.93±0.41 ABd | 1.96±0.22 Ad |
| 生育时期Growth stage | 处理Treatment | 碳氮比C/N ratio | 碳磷比C/P ratio | 氮磷比N/P ratio |
|---|---|---|---|---|
| 分蘖期Tillering | CK | 10.85±0.14 Ad | 119.07±5.05 Ad | 10.98±0.61 Aa |
| S1 | 10.32±0.31 Bd | 118.59±1.91 Ad | 11.50±0.54 Aa | |
| S2 | 10.79±0.02 Ad | 120.38±0.76 Ad | 11.16±0.06 Aa | |
| 拔节期Jointing | CK | 14.14±0.08 Ac | 137.59±3.22 Ac | 9.73±0.17 Bbc |
| S1 | 13.18±0.07 Bc | 137.20±0.29 Ac | 10.41±0.07 Ab | |
| S2 | 13.48±0.30 Bc | 132.46±0.03 Bc | 9.83±0.22 Bb | |
| 抽穗期Heading | CK | 18.47±0.53 ABb | 187.66±1.79 Bb | 10.16±0.19 ABb |
| S1 | 18.97±0.05 Ab | 199.60±3.74 Ab | 10.52±0.17 Ab | |
| S2 | 18.00±0.35 Bb | 177.87±0.22 Cb | 9.88±0.18 Bb | |
| 成熟期Maturity | CK | 28.20±0.75 Ba | 262.09±2.65 Aa | 9.30±0.15 Ac |
| S1 | 31.22±0.29 Aa | 271.13±9.53 Aa | 8.68±0.22 Ac | |
| S2 | 28.14±0.67 Ba | 258.93±17.27 Aa | 9.21±0.84 Ac |
表3 不同处理对水稻叶片生态化学计量特征的影响
Table 3 Effect of different treatments on ecological stoichiometry of rice leaves
| 生育时期Growth stage | 处理Treatment | 碳氮比C/N ratio | 碳磷比C/P ratio | 氮磷比N/P ratio |
|---|---|---|---|---|
| 分蘖期Tillering | CK | 10.85±0.14 Ad | 119.07±5.05 Ad | 10.98±0.61 Aa |
| S1 | 10.32±0.31 Bd | 118.59±1.91 Ad | 11.50±0.54 Aa | |
| S2 | 10.79±0.02 Ad | 120.38±0.76 Ad | 11.16±0.06 Aa | |
| 拔节期Jointing | CK | 14.14±0.08 Ac | 137.59±3.22 Ac | 9.73±0.17 Bbc |
| S1 | 13.18±0.07 Bc | 137.20±0.29 Ac | 10.41±0.07 Ab | |
| S2 | 13.48±0.30 Bc | 132.46±0.03 Bc | 9.83±0.22 Bb | |
| 抽穗期Heading | CK | 18.47±0.53 ABb | 187.66±1.79 Bb | 10.16±0.19 ABb |
| S1 | 18.97±0.05 Ab | 199.60±3.74 Ab | 10.52±0.17 Ab | |
| S2 | 18.00±0.35 Bb | 177.87±0.22 Cb | 9.88±0.18 Bb | |
| 成熟期Maturity | CK | 28.20±0.75 Ba | 262.09±2.65 Aa | 9.30±0.15 Ac |
| S1 | 31.22±0.29 Aa | 271.13±9.53 Aa | 8.68±0.22 Ac | |
| S2 | 28.14±0.67 Ba | 258.93±17.27 Aa | 9.21±0.84 Ac |
| 处理 Treatment | 穗数 Ear number/ (104 hm-2) | 穗粒数 Number of spikes | 千粒重 Thousand seeds weight/g | 结实率 Seeding rate/% | 理论产量 Theoretical yield/(t·hm-2) | 实测产量 Actual yield/ (t·hm-2) |
|---|---|---|---|---|---|---|
| CK | 498.14±22.49 A | 123.30±4.48 A | 24.08±0.81 B | 85.05±0.62 B | 12.47±0.15 B | 11.82±0.16 B |
| S1 | 500.89±25.24 A | 113.16±1.93 B | 24.97±0.15 A | 88.11±0.53 A | 12.31±0.16 B | 11.58±0.18 B |
| S2 | 526.68±20.12 A | 117.72±4.61 AB | 23.90±0.47 B | 87.52±1.72 A | 13.20±0.14 A | 12.52±0.20 A |
表4 不同处理对水稻产量及其构成因素的影响
Table 4 Effects of different treatments on on grain yield and its components
| 处理 Treatment | 穗数 Ear number/ (104 hm-2) | 穗粒数 Number of spikes | 千粒重 Thousand seeds weight/g | 结实率 Seeding rate/% | 理论产量 Theoretical yield/(t·hm-2) | 实测产量 Actual yield/ (t·hm-2) |
|---|---|---|---|---|---|---|
| CK | 498.14±22.49 A | 123.30±4.48 A | 24.08±0.81 B | 85.05±0.62 B | 12.47±0.15 B | 11.82±0.16 B |
| S1 | 500.89±25.24 A | 113.16±1.93 B | 24.97±0.15 A | 88.11±0.53 A | 12.31±0.16 B | 11.58±0.18 B |
| S2 | 526.68±20.12 A | 117.72±4.61 AB | 23.90±0.47 B | 87.52±1.72 A | 13.20±0.14 A | 12.52±0.20 A |
图3 土壤-叶片碳氮磷含量、生态化学计量特征与水稻产量的相关性 C,土壤有机碳;N,土壤全氮;P,土壤全磷;C∶N,土壤碳氮比;C∶P,土壤碳磷比;N∶P,土壤氮磷比;LC,水稻叶片C含量;LN,水稻叶片N含量;LP,水稻叶片P含量;LC∶N,水稻叶片碳氮比;LC∶P,水稻叶片碳磷比;LN∶P,水稻叶片氮磷比;T,理论产量;A,实测产量。“*”“**”分别表示相关性在P<0.05、P<0.01水平下显著。
Fig.3 Correlation within soil-leaf carbon, nitrogen and phosphorus contents, stoichiometric characteristics and rice yield C, Soil organic carbon; N, Soil total nitrogen; P, Soil total phosphorus; C∶N, C/N ratio of soil; C∶P, C/P ratio of soil; N∶P, N/P ratio of soil; LC, C content of rice leaves; LN, N content of rice leaves; LP, P content of rice leaves; LC∶N, C/N ratio of rice leaves; LC∶P, C/P ratio of rice leaves; LN∶P, N/P ratio of rice leaves; T, Theoretical yield; A, Actual yield. “*” “**” represent significant correlations at P<0.05 and P<0.01, respectively.
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