›› 2012, Vol. 24 ›› Issue (1): 0-11.

• 作物科学 •    

3个水稻叶色突变体的遗传分析及基因定位

纪现军1,2,叶胜海2,周涯2,3,修芬连2,3,邓晓梅1,2,尚海漩2,3,刘继云2,3,陈萍萍2,金庆生2,张小明2,*

  

  1. 1杭州师范大学 生命与环境科学学院,浙江 杭州 310036;2浙江省农业科学院 作物与核技术利用研究所,浙江 杭州 310021;3浙江师范大学 化学与生命科学学院,浙江 金华 321004
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-01-24 发布日期:2012-01-24

Genetic analysis and mapping of three rice mutants with yellow leaf trait

JI Xianjun;YE Shenghai;ZHOU Ya;XIU Fenlian;DENG Xiaomei;SHANG Haixuan;LIU Jiyun;CHEN Pingping;JIN Qingsheng;ZHANG Xiaoming;*   

  1. 1College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036,China; 2Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China;3College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-01-24 Published:2012-01-24

摘要:

利用甲基磺酸乙酯对常规粳稻‘秀水09’进行诱变处理,获得了3个黄叶突变体Y 1,Y 2和Y 3,多代自交稳定遗传。突变体与野生型‘秀水09’相比,分蘖减少、植株变矮、苗期叶片表现明显的黄色,随着生长发育的进行,叶片逐渐返绿。遗传分析表明这3个突变体的黄叶性状均受1对隐性核基因控制。利用SSR分子标记,将突变基因Yl1定位在第3号染色体RM282与RM6080之间的7.5 cM范围内,将突变基因Y 2和Y 3定位在第5号染色体分子标记5.43w与RM1237之间,遗传距离分别为2.0 cM和6.8 cM。

关键词: 水稻, 黄叶, 遗传分析, 分子定位

Abstract: Three stably inherited yellow leaf mutants, Yl1, Yl2 and Yl3, were isolated from a japonica rice variety Xiushui 09 treated by ethyl methylsulfonate (EMS). Compared with their wild type Xiushui 09, these mutants showed less tiller and shorter culm, and yellow leaves were observed at the seedling and tilling stages. With the growth of the mutant plants, the leaves turn green. Genetic analysis showed that the yellow leaf traits of the three mutants were all controlled by one pair of recessive nuclear genes. Genetic mapping of the mutant genes were conducted using yellow leaf individuals from the F2 mapping populations of Y 1, Y 2 and Y 3 crossed with indica variety Zhenshan 97. Y 1 was mapped between SSR markers RM282 and RM6080, within genetic distance of 7.5 cM in chromosome 3. While Y 2 and Y 3 were mapped in the same site of chromosome 5 between 5.43w and RM1237, with genetic distances of 2.0 cM and 6.8 cM to each of them, respectively.

Key words: rice, yellow leaf, genetic analysis, mapping