›› 2010, Vol. 22 ›› Issue (1): 0-72.

• 论文 •    

土霉素诱导土壤可培养细菌产生耐药性的研究

章明奎1,2,普锦成1,2   

  1. 1浙江大学 环境与资源学院,土水资源与环境研究所,浙江 杭州 310029;2浙江省亚热带土壤与植物营养重点研究实验室,浙江 杭州 310029
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-25 发布日期:2010-01-25

Cultivable soil bacteria antibiotic resistance induced by oxytetracycline pollution

ZHANG Ming-kui;PU Jin-cheng;   

  1. 1Institute of Soil and Water Resource and Environmental Science,College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310029,China;2Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition,Hangzhou 310029,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-25 Published:2010-01-25

摘要: 通过在土壤中反复添加不同浓度的土霉素进行预处理,采用平板稀释法测定了土壤细菌在含土霉素培养基及普通培养基上的生长情况,用耐药菌发生率作为指标评价了土霉素污染浓度和污染次数对土壤抗土霉素可培养细菌的影响。结果表明,土霉素污染可诱导土壤土霉素抗性细菌的产生,耐药菌发生率与土霉素添加浓度和添加次数有关。当土霉素添加浓度大于4 mg/kg时,一次处理就可诱导土壤可培养细菌产生耐药性;当土霉素浓度为3 mg/kg时,二次处理可诱导土壤可培养细菌产生耐药性;当土霉素浓度为2 mg/kg时,需要5次处理才可诱导土壤可培养细菌产生耐药性;而当土霉素浓度为1 mg/kg时,5次处理均没有明显诱导土壤可培养细菌产生耐药性。研究结果表明,长期施用含土霉素的畜禽粪可导致土壤土霉素抗性细菌的形成和发展。

关键词: 土霉素, 土壤, 耐药性

Abstract: Antibiotics enter agricultural soils by the use of animal excrements as fertilizers. However,it is scarcely known how these antibiotics affect the cultivable soil bacteria antibiotic resistance. Therefore,effect of antibiotic oxytetracycline (OTC) pollution on cultivable soil bacteria antibiotic resistance was studied by addition of different dose of OTC in the soil. Percentage of soil bacteria antibiotic resistance was evaluated by comparing cultivable bacteria number in culture medium spiked with or without OTC. The results showed that soil OTC pollution could increase the cultivable soil bacteria,which was related to the additional dose and times of OTC. The cultivable soil bacteria antibiotic resistance was significantly increased when soil was treated once with OTC at the dose of 4 mg·kg-1,or repeatedly treated twice at the dose of 3 mg·kg-1,or repeatedly treated five times at the dose of 2 mg·kg-1. But,no significant increase in the cultivable soil bacteria antibiotic resistance was found when soil was treated with OTC at the dose of 1 mg·kg-1. The results suggested that long-term application of antibiotics-containing animal manure could improve the development of soil bacteria antibiotic resistance.

Key words: oxytetracycline, soil, antibiotic resistance