Effects of rotation patterns on growth, quality and rhizosphere soil environment of Chrysanthemum morifolium Ramat. cv. Hangbaiju

doi:10.3969/j.issn.1004-1524.20250520

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (8): 1610-1619.DOI: 10.3969/j.issn.1004-1524.20250520

• Horticultural Science • Previous Articles Next Articles

Effects of rotation patterns on growth, quality and rhizosphere soil environment of Chrysanthemum morifolium Ramat. cv. Hangbaiju

SUN Jian¹^,²(), YE Lingzhen³, WANG Chuanbao¹, CAI Long¹, YU Guoliang¹, SUN Yuefang⁴, ZHOU Jiansong⁴, WANG Yin²^,^*()

¹ Zhejiang Institute of Chinese Materia Medica Co., Ltd., Hangzhou 310023, China
² Key Laboratory of Creative Agriculture, Ministry of Agriculture and Rural Affairs, Institute of Rural Development, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
³ Institute of Crop Science, Zhejiang University, Hangzhou 310058, China
⁴ Tongxiang Institute of Agricultural Sciences, Jiaxing Academy of Agricultural Sciences, Tongxiang 314500, Zhejiang, China

Received:2025-07-31 Online:2026-08-25 Published:2026-09-14

Abstract

Abstract:

Long-term continuous cropping of Chrysanthemum morifolium Ramat. cv. Hangbaiju has resulted in soil nutrient imbalance, microbial community disruption, and intensified pest and disease pressure, thereby constraining sustainable industrial development. This study established three rotation patterns: barley (Hordeum vulgare L.)-chrysanthemum, wheat (Triticum aestivum L.)-chrysanthemum, and bok choy [Brassica rapa var. chinensis(L.) Kitam.]-chrysanthemum, using continuous chrysanthemum cropping as the control, to systematically evaluate their effects on the growth, quality and rhizosphere soil environment of Chrysanthemum morifolium. The results showed that in the barley-chrysanthemum rotation, the flower number per plant, number of petals and dry weight of 10 flowers were significantly higher than those in other treatments, with all yield indicators superior to those in other treatments. Although the wheat-chrysanthemum rotation improved individual flower quality, it suppressed early-stage growth, resulting in a survival rate of only 80.63%. The effect of the bok choy-chrysanthemum rotation was similar to that of the control. No significant differences were observed in chlorogenic acid, luteolin-7-O-glucoside and 3,5-O-dicaffeoylquinic acid contents among treatments, all of which met the standards of Chinese Pharmacopoeia. Rotation with gramineous crops significantly increased the contents of soil total nitrogen, ammonium nitrogen and organic matter. Microbial diversity analysis revealed that barley-chrysanthemum and wheat-chrysanthemum rotations exhibited significantly higher Shannon indices compared with the control, with enrichment of beneficial genera including Burkholderia and Phyllobacterium, thereby optimizing rhizosphere microbial community structure. Comprehensive analysis indicated that the barley-chrysanthemum rotation significantly promoted the growth, development and yield formation of Chrysanthemum morifolium by improving soil fertility and optimizing the rhizosphere microbial community. It was a superior rotation pattern for alleviating the continuous cropping obstacles of Chrysanthemum morifolium, providing a theoretical basis and technical support for its sustainable production.

Key words: Chrysanthemum morifolium Ramat. cv. Hangbaiju, rotation pattern, continuous cropping obstacle, rhizosphere microorganism, soil nutrient

CLC Number:

S344.1

SUN Jian, YE Lingzhen, WANG Chuanbao, CAI Long, YU Guoliang, SUN Yuefang, ZHOU Jiansong, WANG Yin. Effects of rotation patterns on growth, quality and rhizosphere soil environment of Chrysanthemum morifolium Ramat. cv. Hangbaiju[J]. Acta Agriculturae Zhejiangensis, 2026, 38(8): 1610-1619.

Figures/Tables 5

References 32

[1]	Cardinale B J, Duffy J E, Gonzalez A, et al. Biodiversity loss and its impact on humanity[J]. Nature, 2012, 486(7401): 59-67.
[2]	Liu Q, Zhao Y X, Li T, et al. Changes in soil microbial biomass, diversity, and activity with crop rotation in cropping systems: a global synthesis[J]. Applied Soil Ecology, 2023, 186: 104815.
[3]	Banerjee S, Walder F, Büchi L, et al. Agricultural intensification reduces microbial network complexity and the abundance of keystone taxa in roots[J]. The ISME Journal, 2019, 13(7): 1722-1736.
[4]	Jing J Y, Cong W F, Bezemer T M. Legacies at work: plant-soil-microbiome interactions underpinning agricultural sustainability[J]. Trends in Plant Science, 2022, 27(8): 781-792.
[5]	Zhao J, Yang Y D, Zhang K, et al. Does crop rotation yield more in China? A meta-analysis[J]. Field Crops Research, 2020, 245: 107659.
[6]	刘智赫, 李冰圳, 张倩倩, 等. 不同轮作方式对北沙参生长及药材质量的影响[J]. 中药材, 2023, 46(2): 272-278.
	Liu Z H, Li B Z, Zhang Q Q, et al. Effects of different crop rotation methods on the growth and quality of Glehnia littoralis[J]. Journal of Chinese Medicinal Materials, 2023, 46(2): 272-278.
[7]	周丹, 韦雪娇, 李开阳, 等. 紫苏轮作对半夏连作根际土壤微生物群落结构、功能及理化性质的影响[J]. 江苏农业科学, 2025, 53(12): 235-249.
	Zhou D, Wei X J, Li K Y, et al. Effects of Perilla rotation on microbial community structure, function and physicochemical properties of rhizosphere soil in Pinellia ternata continuous cropping[J]. Jiangsu Agricultural Sciences, 2025, 53(12): 235-249.
[8]	Zhou X G, Zhang J Y, Khashi u Rahman M, et al. Interspecific plant interaction via root exudates structures the disease suppressiveness of rhizosphere microbiomes[J]. Molecular Plant, 2023, 16(5): 849-864.
[9]	檀国印, 杨志玲, 袁志林, 等. 药用植物连作障碍及其防治途径研究进展[J]. 西北农林科技大学学报(自然科学版), 2012, 40(4): 197-204.
	Tan G Y, Yang Z L, Yuan Z L, et al. Research advances in continuous cropping obstacle in medicinal plants and its management[J]. Journal of Northwest A & F University(Natural Science Edition), 2012, 40(4): 197-204.
[10]	张敏, 谈献和, 张瑜, 等. 中药材连作障碍[J]. 现代中药研究与实践, 2012, 26(1): 83-85.
	Zhang M, Tan X H, Zhang Y, et al. Continuous cropping obstacles of Chinese medicinal materials[J]. Research and Practice on Chinese Medicines, 2012, 26(1): 83-85.
[11]	张冬琳. 中药材与农林生物的间套轮作等复合种植模式[J]. 农业与技术, 2016, 36(16): 2.
	Zhang D L. Compound planting patterns such as intercropping, relay cropping and rotation of Chinese medicinal materials with agricultural and forestry organisms[J]. Agriculture and Technology, 2016, 36(16): 2.
[12]	Lin S, Huangqu J J, Chen T, et al. Effects of different cropping patterns on the physiology and quality of Pseudostellariae heterophylla[J]. International Journal of Agriculture and Biology, 2014, 16(5): 981-985.
[13]	陈冬梅, 陈祥旭, 孙红艳, 等. 麦类作物化感作用种质资源筛选与评价[J]. 福建农林大学学报(自然科学版), 2008, 37(1): 13-17.
	Chen D M, Chen X X, Sun H Y, et al. Screening and evaluation of allelopathic potential in triticeae crops germplasms[J]. Journal of Fujian Agriculture and Forestry University(Natural Science Edition), 2008, 37(1): 13-17.
[14]	方彦杰, 徐银萍. 不同栽培模式对大麦生育期间土壤水分及产量的影响[J]. 干旱地区农业研究, 2015, 33(3): 190-195.
	Fang Y J, Xu Y P. Effects of different cultivation patterns on soil moisture and yield during the barley growth period[J]. Agricultural Research in the Arid Areas, 2015, 33(3): 190-195.
[15]	杨忠义, 段国锋, 马金虎, 等. 黄土丘陵区菊花种植模式的生态及经济效益分析[J]. 中国农学通报, 2014, 30(4): 195-199.
	Yang Z Y, Duan G F, Ma J H, et al. Analysis on the ecological and economic benefits of cultivation patterns of Chrysanthemum morifolium Ramat. in hilly loess region[J]. Chinese Agricultural Science Bulletin, 2014, 30(4): 195-199.
[16]	Wu H, Pratley J, Lemerle D, et al. Allelopathy in wheat (Triticum aestivum)[J]. Annals of Applied Biology, 2001, 139(1): 1-9.
[17]	Mehri S, Vahid R S, H Torabi Sirchisup M. Allelopathic appraisal effects of straw extract wheat varieties on the growth of corn[J]. African Journal of Biotechnology, 2010, 9(48): 8154-8160.
[18]	Matthiessen J N, Kirkegaard J A. Biofumigation and enhanced biodegradation: opportunity and challenge in soilborne pest and disease management[J]. Critical Reviews in Plant Sciences, 2006, 25(3): 235-265.
[19]	Chen S L, Yu H, Luo H M, et al. Conservation and sustainable use of medicinal plants: problems, progress, and prospects[J]. Chinese Medicine, 2016, 11: 37.
[20]	Yang L, Wen K S, Ruan X, et al. Response of plant secondary metabolites to environmental factors[J]. Molecules, 2018, 23(4): 762.
[21]	王籽懿, 黄修梅, 杨玉荣, 等. 不同轮作模式对东北黑土地影响的研究进展[J]. 江苏农业科学, 2024, 52(11): 27-34.
	Wang Z Y, Huang X M, Yang Y R, et al. Research progress on effects of different rotation patterns on black soil in Northeast China[J]. Jiangsu Agricultural Sciences, 2024, 52(11): 27-34.
[22]	焦旭升, 魏春雷, 杨春, 等. 中药材轮作栽培的机理及模式研究进展[J]. 寒旱农业科学, 2024(5): 405-409.
	Jiao X S, Wei C L, Yang C, et al. Research progress on the mechanisms and models of the rotation cultivation in Chinese traditional medicinal crops[J]. Journal of Cold-Arid Agricultural Sciences, 2024(5): 405-409.
[23]	李冉, 李精华, 林生威, 等. 稻豆轮作对土壤理化性质及微生物群落的影响[J]. 热带生物学报, 2024, 15(2): 157-164.
	Li R, Li J H, Lin S W, et al. Effects of rice-soybean-rice rotation on soil physicochemical properties and microbial community[J]. Journal of Tropical Biology, 2024, 15(2): 157-164.
[24]	Tiemann L K, Grandy A S, Atkinson E E, et al. Crop rotational diversity enhances belowground communities and functions in an agroecosystem[J]. Ecology Letters, 2015, 18(8): 761-771.
[25]	刘彦伶, 李渝, 张雅蓉, 等. 长期不同轮作下黄壤微生物量碳季节变化特征与差异[J]. 环境科学, 2025, 46(12): 8062-8070.
	Liu Y L, Li Y, Zhang Y R, et al. Seasonal variation and differences in microbial biomass carbon in yellow soil under different long-term crop rotation patterns[J]. Environmental Science, 2025, 46(12): 8062-8070.
[26]	Kumar A, Dubey A. Rhizosphere microbiome: Engineering bacterial competitiveness for enhancing crop production[J]. Journal of Advanced Research, 2020, 24: 337-352.
[27]	Estrada-De Los Santos P, Bustillos-Cristales R, Caballero-Mellado J. Burkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution[J]. Applied and Environmental Microbiology, 2001, 67(6): 2790-2798.
[28]	Contesto C, Milesi S, Mantelin S, et al. The auxin-signaling pathway is required for the lateral root response of Arabidopsis to the rhizobacterium Phyllobacterium brassicacearum[J]. Planta, 2010, 232(6): 1455-1470.
[29]	Venter Z S, Jacobs K, Hawkins H J. The impact of crop rotation on soil microbial diversity: a meta-analysis[J]. Pedobiologia, 2016, 59(4): 215-223.
[30]	Barka E A, Vatsa P, Sanchez L, et al. Taxonomy, physiology, and natural products of Actinobacteria[J]. Microbiology and Molecular Biology Reviews, 2016, 80(1): 1-43.
[31]	丁亚茹, 陈玉蓝, 林正全, 等. 不同发病率烟田根际土壤微生物群落组成研究[J]. 中国烟草科学, 2020, 41(1): 67-73.
	Ding Y R, Chen Y L, Lin Z Q, et al. Study on microbial community composition in rhizosphere soil of tobacco fields with different disease incidence rates[J]. Chinese Tobacco Science, 2020, 41(1): 67-73.
[32]	Kielak A M, Barreto C C, Kowalchuk G A, et al. The ecology of Acidobacteria: moving beyond genes and genomes[J]. Frontiers in Microbiology, 2016, 7: 744.

处理 Treatment	成活率/% Survival rate/%	总分枝数 Total number of branches	单株开花数 Number of flowers per plant	单株花苞数 Number of flower buds per plant	单丛总花数 Total number of single cluster flowers	花序直径/mm Inflorescence diameter/mm
CK	95.63±2.22 a	23.66±1.57 a	441.33±8.76 b	369.33±14.56 a	808.54±22.15 a	43.98±0.88 a
QCJH	96.74±1.58 a	22.33±1.86 a	432.00±9.95 b	366.33±10.07 a	794.48±23.71 a	44.77±0.59 a
DMJH	95.43±1.12 a	23.33±2.71 a	460.09±6.25 a	359.00±13.27 a	821.35±19.16 a	45.98±0.84 a
XMJH	80.63±3.54 b	18.66±1.24 b	362.50±23.56 c	324.59±8.94 b	689.52±24.55 b	44.89±0.97 a
处理 Treatment	花芯直径/mm Diameter of the flower core/mm	舌状花数 Number of petals	10朵花鲜重/g Fresh weight of 10 flowers/g	10朵花干重/g Dry weight of 10 flowers/g	折干率/% Drying rate/%
CK	11.78±0.63 a	226.33±5.43 c	20.96±0.56 a	2.80±0.07 c	13.37±0.11 b
QCJH	11.76±0.65 a	222.67±8.21 c	20.23±0.57 a	2.68±0.10 c	13.25±0.11 b
DMJH	11.65±0.52 a	245.00±7.65 a	22.15±0.47 a	3.07±0.04 a	13.86±0.08 a
XMJH	11.67±0.61 a	234.67±6.47 b	21.23±0.51 a	2.94±0.02 b	13.84±0.05 a

处理 Treatment	成活率/% Survival rate/%	总分枝数 Total number of branches	单株开花数 Number of flowers per plant	单株花苞数 Number of flower buds per plant	单丛总花数 Total number of single cluster flowers	花序直径/mm Inflorescence diameter/mm
CK	95.63±2.22 a	23.66±1.57 a	441.33±8.76 b	369.33±14.56 a	808.54±22.15 a	43.98±0.88 a
QCJH	96.74±1.58 a	22.33±1.86 a	432.00±9.95 b	366.33±10.07 a	794.48±23.71 a	44.77±0.59 a
DMJH	95.43±1.12 a	23.33±2.71 a	460.09±6.25 a	359.00±13.27 a	821.35±19.16 a	45.98±0.84 a
XMJH	80.63±3.54 b	18.66±1.24 b	362.50±23.56 c	324.59±8.94 b	689.52±24.55 b	44.89±0.97 a
处理 Treatment	花芯直径/mm Diameter of the flower core/mm	舌状花数 Number of petals	10朵花鲜重/g Fresh weight of 10 flowers/g	10朵花干重/g Dry weight of 10 flowers/g	折干率/% Drying rate/%
CK	11.78±0.63 a	226.33±5.43 c	20.96±0.56 a	2.80±0.07 c	13.37±0.11 b
QCJH	11.76±0.65 a	222.67±8.21 c	20.23±0.57 a	2.68±0.10 c	13.25±0.11 b
DMJH	11.65±0.52 a	245.00±7.65 a	22.15±0.47 a	3.07±0.04 a	13.86±0.08 a
XMJH	11.67±0.61 a	234.67±6.47 b	21.23±0.51 a	2.94±0.02 b	13.84±0.05 a

处理 Treatment	绿原酸含量 Chlorogenic acid content	木犀草苷含量 Luteolin-7-O-glucoside content	3,5-O-二咖啡酰基奎宁酸含量 3,5-O-dicaffeoylquinic acid content
CK	4.82±0.23 a	2.35±0.13 a	7.96±0.32 a
QCJH	4.74±0.26 a	2.21±0.15 a	7.51±0.27 a
DMJH	4.47±0.58 a	2.16±0.14 a	8.25±0.23 a
XMJH	4.55±0.17 a	2.13±0.22 a	8.17±0.19 a

处理 Treatment	绿原酸含量 Chlorogenic acid content	木犀草苷含量 Luteolin-7-O-glucoside content	3,5-O-二咖啡酰基奎宁酸含量 3,5-O-dicaffeoylquinic acid content
CK	4.82±0.23 a	2.35±0.13 a	7.96±0.32 a
QCJH	4.74±0.26 a	2.21±0.15 a	7.51±0.27 a
DMJH	4.47±0.58 a	2.16±0.14 a	8.25±0.23 a
XMJH	4.55±0.17 a	2.13±0.22 a	8.17±0.19 a

处理 Treatment	全氮含量/ (mg·kg^-1) Total N content/ (mg·kg^-1)	有效磷含量/ (mg·kg^-1) Available P content/ (mg·kg^-1)	速效钾含量/ (mg·kg^-1) Available K content/ (mg·kg^-1)	铵态氮含量/ (mg·kg^-1) N${\mathrm{H}}_{4}^{+}$-N content/ (mg·kg^-1)	有机质含量/ (g·kg^-1) Organic matter content/ (g·kg^-1)	pH值 pH value
CK	1 440.23±8.97 b	35.09±0.87 b	125.78±0.77 b	41.42±0.56 b	27.37±1.22 b	7.57±0.08 a
QCJH	1 430.35±10.53 b	35.18±0.66 b	125.88±0.68 b	41.83±2.35 b	26.71±1.54 b	7.35±0.12 a
DMJH	1 550.67±9.96 a	36.82±0.97 a	124.91±0.75 b	48.23±1.79 a	30.71±1.76 a	7.35±0.10 a
XMJH	1 588.09±8.98 a	36.57±1.08 a	126.71±0.15 a	49.67±2.08 a	30.36±1.43 a	7.43±0.05 a

Effects of rotation patterns on growth, quality and rhizosphere soil environment of Chrysanthemum morifolium Ramat. cv. Hangbaiju

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