Behavioral regulation of the interaction between aggregation pheromone and plant volatiles on Megalurothrips usitatus

doi:10.3969/j.issn.1004-1524.20240731

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (3): 532-540.DOI: 10.3969/j.issn.1004-1524.20240731

• Plant Protection • Previous Articles Next Articles

Behavioral regulation of the interaction between aggregation pheromone and plant volatiles on Megalurothrips usitatus

LI Xiaowei(), LU Xinxin, PENG Xiaohua, LYU Yaobin()

State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2024-08-13 Online:2026-03-25 Published:2026-04-17
Contact: LYU Yaobin

Abstract

Abstract:

Megalurothrips usitatus is an important pest on leguminous crops, and has been reported to cause tremendous losses to leguminous crops industry in China. Aggregation pheromone and plant volatiles based behavioral manipulation techniques are promising eco-friendly control tactics for M. usitatus. The aim of this study was to screen plant volatiles with attractive activities to M. usitatus, investigate the interaction between aggregation pheromone and plant volatiles, and develop synergist blends. The results showed that 0.1 μL·mL^-1 linalool, 1.0 μL·mL^-1 methyl isonicotinate and 1.0 μL·mL^-1 eugenol were attractive to M. usitatus female adults. When the three volatile compounds at the specified doses were mixed with the aggregation pheromone components of M. usitatus at the optimal luring dose [5.00 ng·μL^-1 (E, E)-farnesyl acetate] respectively, eugenol and linalool showed antagonistic effects against (E,E)-farnesylacetate. By contrast, methyl isonicotinate showed no antagonistic effect on (E,E)-farnesylacetate, indicating that the combination of 5.00 ng·μL^-1 (E, E)-farnesyl acetate and 1.0 μL·mL^-1 methyl isonicotinate can be used as an optimized ratio for the bait of M. usitatus. This study screened out three plant volatiles which can significantly attract M. usitatus, clarified the interaction between plant volatiles and aggregation pheromone, which provided the basis for the behavior regulation technology of M. usitatus based on information technology and provided references for the development of efficient M. usitatus attractants.

Key words: Megalurothrips usitatus, aggregation pheromone, plant volatile, behavioral regulation

CLC Number:

S435

LI Xiaowei, LU Xinxin, PENG Xiaohua, LYU Yaobin. Behavioral regulation of the interaction between aggregation pheromone and plant volatiles on Megalurothrips usitatus[J]. Acta Agriculturae Zhejiangensis, 2026, 38(3): 532-540.

Figures/Tables 5

Fig.1 Olfactory selection of M. usitatus female adults to different doses of (E,E)-farnesyl acetate ** indicates a highly significant (p<0.01) difference between the treatment group and the control, and NS indicates no statistically significant difference. The same as below.

Fig.2 Olfactory selection of M. usitatus female adults to 5 plant volatiles at different doses * and ***indicate significant (p<0.05) and extremely significant (p<0.001) differences between treatment group and control, NS mean there was no significant difference. The same as below.

Fig.3 Olfactory selection of M. usitatus female adults to the mixture of aggregation pheromone compound and eugenol AP, Aggregation pheromone compound; E, Enugenol. The same as below.

Fig.4 Response of female adults of Megalurothrips usitatus to the mixture of aggregation pheromone compound and linalool L, Linalool.

Fig.5 Response of M. usitatus female adults to the mixture of aggregation pheromone compound and methyl isonicotinate M, Methyl isonicotinate.

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Behavioral regulation of the interaction between aggregation pheromone and plant volatiles on Megalurothrips usitatus

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